Establishment of mouse-directed HCV infection models

• Project/Area Number: 22659145
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SAKAMOTO Naoya 東京医科歯科大学, 大学院・医歯学総合研究科, 寄附講座教員 (10334418)
• Co-Investigator(Kenkyū-buntansha): KAKINUMA Sei 東京医科歯科大学, 大学院・医歯学総合研究科, 寄付講座教員 (30372444) ; NAKAGAWA Mina 東京医科歯科大学, 医学部附属病院, 助教 (30401342)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: HCV / CD81 / HCV-JFH1培養系

Research Abstract

In our present study, we constructed two fluorescence protein-tagged recombinant JFH1 virus clones, JFH1-EYFP and JFH1-AsRed, as well as two corresponding clones with adaptive mutations, JFH1-EYFP mutant and JFH1-AsRed mutant, that and were as effective as JFH1 in producing infectious virus particles, and investigated their viral infection life cycles. After infection of the fluorescence-tagged mutant viruses, infected cells increased exponentially. In cells, EYFP or AsRed and NS5A were expressed as a fusion protein and co-localized in core proteins. The rate of the cell. cell spread was dependent on the cell densities with a maximum of 102. 5/day. Treatment of cells with interferon or a protease inhibitor suppressed expansion of virus-positive cells. Taken together, these results indicate that fluorescence-tagged HCV is a useful tool to study virus infection life cycles and to assist in the search for novel antiviral compounds.

Research Products

• [Journal Article] Suppression of hepatitis C virus replicon by adenovirus vector-mediated expression of tough decoy RNA against miR-122a. Virus Research (2012)
  • Author(s): Sakurai F, Furukawa N, Higuchi M, Okamoto S, Ono K, Yoshida T, Kondoh M, Yagi K, Sakamoto N, Katayama K, Mizuguchi H
  • Journal Title: EPub ahead of Print Volume: 165 Issue: 2 Pages: 214-218
  • DOI: 10.1016/j.virusres.2012.02.003
  • Peer Reviewed
• [Journal Article] Identification of novel N-(morpholine-4-carbonyloxy) amidine compounds as potent inhibitors against hepatitis C virus replication (2012)
  • Author(s): Kusano-Kitazume A, Sakamoto N(equalcontribution), Okuno Y, Sekine-Osajima Y, Nakagawa M, Kakinuma S, Kiyonashi K, Nitta S, Murakawa M, Azuma S, Nishimura-Sakurai Y, Hagiwara M, Watanabe M
  • Journal Title: Antimicrob Agent Chemother Volume: 56(3) Pages: 1315-1323
• [Journal Article] Inhibition of hepatitis C virus NS5B polymerase by S-trityl-L-cysteine derivatives (2012)
  • Author(s): Kaushik-Basu N, Sakamoto N
  • Journal Title: Eur J Med Chem Volume: 49 Pages: 191-199
  • DOI: 10.1016/j.ejmech.2012.01.010
  • Peer Reviewed
• [Journal Article] Age and total ribavirin dose are independent predictors of relapse after interferon therapy in chronic hepatitis C revealed by data mining analysis (2012)
  • Author(s): Kurosaki M, Kakinuma S, Sakamoto N, Nakagawa M, et al.
  • Journal Title: Antivir Ther Volume: 17(1) Pages: 35-43
  • Peer Reviewed
• [Journal Article] Data mining model using simple and readily available factors could identify patients at high risk for hepatocellular carcinoma in chronic hepatitis C (2012)
  • Author(s): Kurosaki M, Sakamoto N, Nakagawa M, et al.
  • Journal Title: J Hepatol Volume: 56(3) Issue: 3 Pages: 602-608
  • DOI: 10.1016/j.jhep.2011.09.011
  • Peer Reviewed
• [Journal Article] Use of human hepatocyte-like cells derived from induced pluripotent stem cells as a model for hepatocytes in hepatitis C virus infection (2011)
  • Author(s): Yoshida T, Takayama K, Kondoh M, Sakurai F, Tani H, Sakamoto N, Matsuura Y, Mizuguchi H, Yagi K
  • Journal Title: Biochem Biophys Res Commun Volume: 416(1-2) Pages: 119-124
• [Journal Article] Adenovirus vector-mediated assay system for hepatitis C virus replication (2011)
  • Author(s): Yoshida T, Kondoh M, Ojima M, Mizuguchi H, Yamagishi Y, Sakamoto N, Yagi K.
  • Journal Title: Nucleic Acids Res Volume: 39(10)
• [Journal Article] Analysis of interferon signaling by infectious hepatitis C virus clones with substitutions of core amino acids 70 and 91 (2011)
  • Author(s): Funaoka Y, Sakamoto N(equal contribution), Nakagawa M, Kakinuma S, Suda S, Watanabe T, Nitta S, Kitazume A, Kiyohashi K, Murakawa M, Azuma S, Tsuchiya K, and Watanabe M
  • Journal Title: J Virol Volume: 85(12) Pages: 5986-5994
• [Journal Article] Studies on virus kinetics using infectious fluorescence-tagged hepatitis C virus cell culture (2011)
  • Author(s): Yamamoto M, Sakamoto N, Nakamura T, Itsui Y, Nakagawa M, Nishimura-Sakurai Y, Kakinuma S, Azuma S, Tsuchiya K, Kato T, Wakita T, Watanabe M
  • Journal Title: Hepatol Res Volume: 41 Pages: 258-269
• [Journal Article] Association between lipid accumulation and the cannabinoid system in Huh7 cells expressing HCV genes (2011)
  • Author(s): Toyoda M, Kitaoka A, Machida K, Nishinakagawa T, Yada R, Kohjima M, Kato M, Kotoh K, Sakamoto N, Shiota G, Nakamuta M, Nakashima M, Enjoji M
  • Journal Title: Int J Mol Med Volume: 27 Pages: 619-624
• [Journal Article] シクロフィリン阻害剤 (2011)
  • Author(s): 中川美奈、坂本直哉、渡辺守
  • Journal Title: 肝胆膵 Volume: 62 Pages: 403-412
• [Journal Article] Association of gene expression involving innate immunity and genetic variation in IL28B with antiviral response (2011)
  • Author(s): Asahina Y, Sakamoto N, et al
  • Journal Title: Hepatology Volume: 55(1) Issue: 1 Pages: 20-29
  • DOI: 10.1002/hep.24623
  • Peer Reviewed
• [Journal Article] Analysis of the complete open reading frame of hepatitis C virus in genotype 2a infection reveals critical sites influencing the response to peginterferon and ribavirin therapy (2011)
  • Author(s): Kadokura M, Nakagawa M, Sakamoto N, Watanabe M, et al.
  • Journal Title: Hepatol Int Volume: 5(3) Issue: 3 Pages: 789-799
  • DOI: 10.1007/s12072-011-9267-x
  • Peer Reviewed
• [Journal Article] A new method for induced fit docking (GENIUS) and its application to virtual screening of novel HCV NS3-4A protease inhibitors (2011)
  • Author(s): Takaya D, Sakamoto N, et al.
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 19(22) Issue: 22 Pages: 6892-6905
  • DOI: 10.1016/j.bmc.2011.09.023
  • Peer Reviewed
• [Journal Article] Serum interleukin-6 levels correlate with resistance to treatment of chronic hepatitis C infection with pegylated-interferon-alpha2b plus ribavirin (2011)
  • Author(s): Ueyama M, Nakagawa M, Sakamoto N, et al.
  • Journal Title: Antivir Ther Volume: 16 Pages: 1081-1091
  • Peer Reviewed
• [Journal Article] Genome-wide association study identified ITPA/DDRGK1 variants reflecting thrombocytopenia in pegylated interferon and ribavirin therapy for chronic hepatitis C (2011)
  • Author(s): Tanaka Y, Sakamoto N, et al.
  • Journal Title: Human Molecular Genetics Volume: 20(17) Issue: 17 Pages: 3507-3516
  • DOI: 10.1093/hmg/ddr249
  • Peer Reviewed
• [Journal Article] Pretreatment Prediction of Anemia Progression by Peginterferon Plus Ribavirin Therapy in Chronic Hepatitis C : Decision-Tree Analysis (2011)
  • Author(s): Hiramatsu N, Sakamoto N, et al.
  • Journal Title: J Gastroenterol Volume: 46(9) Issue: 9 Pages: 1111-1119
  • DOI: 10.1007/s00535-011-0412-z
  • Peer Reviewed
• [Journal Article] Analysis of the complete open reading frame of genotype 2b hepatitis C virus in association with the response to peginterferon and ribavirin therapy (2011)
  • Author(s): Kadokura M, Nakagawa M, Sakamoto N, Watanabe M, et al.
  • Journal Title: PLoS One Volume: 6(9) Issue: 9 Pages: e24514-e24514
  • DOI: 10.1371/journal.pone.0024514
  • Peer Reviewed
• [Journal Article] Pretreatment prediction of response to peginterferon plus ribavirin therapy in genotype 1 chronic hepatitis C using data mining analysis (2011)
  • Author(s): Kurosaki M, Sakamoto N, et al.
  • Journal Title: J Gastroenterol Volume: 46(3) Issue: 3 Pages: 401-409
  • DOI: 10.1007/s00535-010-0322-5
  • NAID: 10029028096
  • Peer Reviewed
• [Journal Article] Adenovirus vector-mediated assay system for hepatitis C virus replication (2011)
  • Author(s): Yoshida T, Sakamoto N, et al
  • Journal Title: Nucleic Acids Res Volume: 39(10) Issue: 10 Pages: e64-e64
  • DOI: 10.1093/nar/gkr047
  • Peer Reviewed
• [Journal Article] Inhibitory effect of a triterpenoid compound, with or without alpha interferon, on hepatitis C virus infection (2011)
  • Author(s): Watanabe T, Sakamoto N, Nakagawa M, Azuma S, Watanabe M, et al.
  • Journal Title: Antimicrob Agents Chemother Volume: 55(6) Issue: 6 Pages: 2537-2545
  • DOI: 10.1128/aac.01780-10
  • Peer Reviewed
• [Journal Article] Association of IL28B variants with response to pegylated-interferon alpha plus ribavirin combination therapy reveals intersubgenotypic differences between genotypes 2a and 2b (2011)
  • Author(s): Sakamoto N, Nakagawa M, Azuma S, Watanabe M, et al.
  • Journal Title: J Med Virol Volume: 83 Issue: 5 Pages: 871-878
  • DOI: 10.1002/jmv.22038
  • Peer Reviewed
• [Journal Article] Cholestatic liver fibrosis and toxin-induced fibrosis are exacerbated in matrix metalloproteinase-2 deficient mice (2011)
  • Author(s): Onozuka I, Sakamoto N, Nakagawa M, Watanabe M, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 406 Issue: 1 Pages: 134-140
  • DOI: 10.1016/j.bbrc.2011.02.012
  • Peer Reviewed
• [Journal Article] Pre-treatment prediction of response to pegylated-interferon plus ribavirin for chronic hepatitis C using genetic polymorphism in IL28B and viral factors (2011)
  • Author(s): Kurosaki M, Sakamoto N, Nakagawa M, Watanabe M, et al.
  • Journal Title: J Hepatol Volume: 54 Issue: 3 Pages: 439-448
  • DOI: 10.1016/j.jhep.2010.07.037
  • Peer Reviewed
• [Journal Article] Sequences in the interferon sensitivity-determining region and core region of hepatitis C virus impact pretreatment prediction of response to PEG-interferon plus ribavirin : data mining analysis (2011)
  • Author(s): Kurosaki M, Sakamoto N, Nakagawa M, et al.
  • Journal Title: J Med Virol Volume: 83 Issue: 3 Pages: 445-452
  • DOI: 10.1002/jmv.22005
  • Peer Reviewed
• [Journal Article] Association between lipid accumulation and the cannabinoid system in Huh7 cells expressing HCV genes.. (2011)
  • Author(s): Toyoda M, Kitaoka A, Machida K, Nishinakagawa T, Yada R, Kohjima M, Kato M, Kotoh K, Sakamoto N, et al.
  • Journal Title: Int J Mol Med Volume: (EPub ahead of print)
  • Peer Reviewed
• [Journal Article] Serum interleukin-6 levels can predict resistance to treatment of chronichepatitis C infection with pegylated-interferon alpha 2b plus ribavirin. (2011)
  • Author(s): Mayumi Ueyama, Mina Nakagawa, Naova Sakamoto, Seishin Azuma, Mamoru Watanabe, et al.
  • Journal Title: Antiviral Therapy Volume: (in press)
  • Peer Reviewed
• [Journal Article] Adenovirus vector-mediated assay system for Hepatitis C virus replication. (2011)
  • Author(s): Takeshi Yoshida, Masuo Kondoh, Manabu Ojima, Hiroyuki Mizuguchi, Naoya Sakamoto
  • Journal Title: Nucl Acid Res Volume: (EPub ahead ofprint)
  • Peer Reviewed
• [Journal Article] Association of IL28B polymorphism with response to pegylated-interferonalpha plus ribavirin combination therapy in patients with chronic genotype 2 hepatitis C. (2011)
  • Author(s): Naoya Sakamoto, et al.
  • Journal Title: J Medical Virol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Studies on virus kinetics using infectious fluorescence-tagged hepatitis Cvirus cell culture. (2011)
  • Author(s): Machi Yamamoto, Naoya Sakamoto, et al.
  • Journal Title: Hepatology Research Volume: (in press)
  • Peer Reviewed
• [Journal Article] Sequences in the Interferon Sensitivity Determining Region and Core Region of Hepatitis C Virus Impact Pretreatment Prediction of Response toPeg-interferon Plus Ribavirin : Data Mining Analysis. (2011)
  • Author(s): Masayuki Kurosaki, Naoya Sakamoto, Manabu Iwasaki, Minoru Sakamoto, et al.
  • Journal Title: J Medical Virol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Pretreatment Prediction of response to peginterferon plus ribavirin inchronic hepatitis C using data mining analysis. (2011)
  • Author(s): Masayuki Kurosaki, Naoya Sakamoto, et al.
  • Journal Title: J Gastroenterol Volume: (in press)
  • Peer Reviewed
• [Journal Article] IL-6-mediated intersubgenotypic variation of interferon sensitivity in hepatitis C virus genotype 2a/2b chimeric clones (2010)
  • Author(s): Suda G, Sakamoto N, Itsui Y, Nakagawa M, Tasaka-Fujita M, Funaoka Y, Watanabe T, Nitta S, Kiyohashi K, Azuma S, Kakinuma S, Tsuchiya K, Imamura M, Hiraga N, Chayama K, Watanabe M
  • Journal Title: Virology Volume: 407 Pages: 80-90
• [Journal Article] Cell culture and in vivo analyses of cytopathic hepatitis C virus mutants (2010)
  • Author(s): Mishima K, Sakamoto N, Sekine-Osajima Y, Nakagawa M, Itsui Y, Azuma S, Kakinuma S, Kiyohashi K, Kitazume A, Tsuchiya K, Imamura M, Hiraga N, Chayama K, Wakita T, Watanabe M
  • Journal Title: Virology Volume: 405 Pages: 361-369
• [Journal Article] HCVの複製・増殖機構と遺伝子型・遺伝子変異 (2010)
  • Author(s): 筬島裕子、中川美奈、坂本直哉
  • Journal Title: 日本臨床 Volume: 69 Pages: 52-58
• [Journal Article] Pretreatment Prediction of Response to Pegylated-Interferon Plus Ribavirinfor Chronic Hepatitis C using Genetic Polymorphism in IL28B and ViralFactors. (2010)
  • Author(s): Masayuki Kurosaki, Yasuhito Tanaka, Nao Nishida, Masaya Sugiyama, Naoya Sakamoto, et al.
  • Journal Title: J Hepatol Volume: (EPub)
  • Peer Reviewed
• [Journal Article] ITPA gene variant protects against anemia induced by pegylated interferon-alfa and ribavirin therapy for Japanese patients with chronichepatitis C. (2010)
  • Author(s): Naova Sakamoto, Yasuhito Tanaka, et al.
  • Journal Title: Hepatology Research Volume: 40 Pages: 1063-1071
  • Peer Reviewed
• [Journal Article] IL-6-mediated intersubgenotypic variation of interferon sensitivity inhepatitis C virus genotype 2a/2b chimeric clones. (2010)
  • Author(s): Goki Suda, Naova Sakamoto, et al.
  • Journal Title: Virology Volume: 407 Pages: 80-90
  • Peer Reviewed
• [Journal Article] Inhibition of HCV replication by a specific inhibitor of serin-arginine-richprotein kinase. (2010)
  • Author(s): Yuko Karakama, Naoya Sakamoto, et al.
  • Journal Title: Antimicrobial Agent and Chemotherapy Volume: 54 Pages: 3179-3186
  • Peer Reviewed
• [Journal Article] Cell culture and in-vivo analyses of plaque-derived cytopathic hepatitis Cvirus mutants. (2010)
  • Author(s): Kako Mishima, Naoya Sakamoto, et al
  • Journal Title: Virology Volume: 405 Pages: 361-369
  • Peer Reviewed
• [Journal Article] Mutations in the Interferon Sensitivity Determining Region and virological response to combination therapy with Pegylated-interferon alpha 2b plusribavirin in patients with chronic hepatitis C-1b infection. (2010)
  • Author(s): Mina Nakagawa, Naoya Sakamoto, et al.
  • Journal Title: J Gastroenterol Volume: 45 Pages: 656-665
  • Peer Reviewed
• [Journal Article] Comparison of HCV-associated gene expression and cell signaling pathways in cells with or without HCV replicon and in replicon-cured cells. (2010)
  • Author(s): Yuki Nishimura-Sakurai, Naoya Sakamoto, et al.
  • Journal Title: J Gastroentorol Volume: 45 Pages: 523-536
  • NAID: 10027216127
  • Peer Reviewed
• [Journal Article] Curcumin inhibits hepatitis C virus replication via suppressing the Akt-SREBP-1 pathway. (2010)
  • Author(s): KyeongJin Kim, Kook Hwan Kim, Hye Young Kim, Hyun Kook Cho, Naova Sakamoto., et al.
  • Journal Title: FEBS Letter Volume: 584 Pages: 707-712
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus replication by chloroquine targeting virus-associated autophagy. (2010)
  • Author(s): Tomokazu Mizui, Shunhei Yamashina, Isei Tanida, Motoki Takashima, Naova Sakamoto, et al.
  • Journal Title: J Gastroenterol Volume: 5 Pages: 195-203
  • NAID: 10027214573
  • Peer Reviewed
• [Presentation] A high-content screening assay using infectious fluorescence-tagged hepatitis C virus reveals candidates for small molecule inhibitors of viral entry (2011)
  • Author(s): Kusano-Kitazume A, Sakamoto N, Okuno Y, Yamamoto M, Sekine-Osajima Y, Nakagawa M, Kakinuma S, Kiyohashi K, Nitta S, Murakawa M, Hagiwara M, Watanabe M
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco, CA.(Poster# 383)
  • Year and Date: 2011-11-04
• [Presentation] ITPA gene variant protects against treatment-induced anemia and improves viral clearance by pegylated interferon-alfa and ribavirin therapy in chronic hepatitis C patients (2011)
  • Author(s): Sakamoto N, Nakagawa M, Kakinuma S, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] MMP-2 and MMP-14 derived from donor cells enhance therapeutic efficacy of liver cell transplantation in mice (2011)
  • Author(s): Kakinuma S, Sakamoto N, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] A high-content screening assay using infectious fluorescence-tagged hepatitis C virus reveals candidates for small molecule inhibitors of viral entry (2011)
  • Author(s): Kusano-Kitazume A, Sakamoto N, Nakagawa M, Kakinuma S, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Loww of Wnt5A promotes biliary differentiation of murine hepatic stem/progenitor cells (2011)
  • Author(s): Kiyohashi K, Kakinuma S, Sakamoto N, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Antiviral effect of a novel interferon-inducible protein, IFI-27, against hepatitis C virus replication (2011)
  • Author(s): Itsuil Y, Sakamoto N, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] IL-6-mediated intersubgenotypic variation of interferon sensitivity in hepatitis C virus genotype 2a/2b chimeric clones (2011)
  • Author(s): Suda G, Sakamoto N, Nakagawa M, Kakinuma S, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Prediction model of ribavirin-induced anemia incorporating ITPA genotype could identify chronic hepatitis C patients at high risk of relapse among virological responders to pegylated-interferon and ribavirin (2011)
  • Author(s): Kurosaki M, Sakamoto N, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] IL28B and ITPA gene variants correlate with treatment effcacy in pegylated-interferon plus ribavirin therapy for chronic hepatitis C (2011)
  • Author(s): Matsuura K, Sakamoto N, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] Mutations in the interferon sensitivity determining region of HCV, age and total ribavirin dose is an independent predictor of relapse among early virological responders to peg-interferon plus ribavirin therapy (2011)
  • Author(s): Kurosaki M, Sakamoto N, Kakinuma S, Nakagawa M, et al.
  • Organizer: 62th. Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: San Francisco
  • Year and Date: 2011-11-04
• [Presentation] EYFP発現HCV培養系を用いたHigh-content screening assayによるウイルス侵入阻害剤の同定.(ポスターP26) (2011)
  • Author(s): 北詰晶子、坂本直哉、奥野友紀子、中川美奈、柿沼晴、山本満千、幾世橋佳、新田沙由梨、村川美也子、萩原正敏、渡辺守
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-20
• [Presentation] Development of an RNA polymerase I-driven adenoviral vector and its application in an HCV replication assay (2011)
  • Author(s): Yoshida T, Sakamoto N, et al.
  • Organizer: 18th. International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] Analysis of interferon signaling by infectious hepatitis C virus clones with substitutions of core amino acids 70 and 91 (2011)
  • Author(s): Sakamoto N, Nakagawa M, Kakinuma S, et al.
  • Organizer: 18th. International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] HCV-NS4B targets STING and abrogates RIG-I-mediated type-I interferon-dependent innate immune response (2011)
  • Author(s): Nitta S, Sakamoto N, Nakagawa M, Kakinuma S, et al.
  • Organizer: 18th. International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Seattle
  • Year and Date: 2011-09-08
• [Presentation] Cell based high-throughput screeningによる新規抗ウイルス小分子の同定及びその作用機構の解析 (2011)
  • Author(s): 北詰晶子、坂本直哉、奥野友紀子、筬島裕子、中川美奈、柿沼晴、植山真由美、小野塚泉、船岡祐介、渡辺貴子、幾世橋佳、新田沙由梨、村川美也子、萩原正敏、渡辺守
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京(一般口演, O-156)
  • Year and Date: 2011-06-03
• [Presentation] 蛍光蛋白発現HCV培養系を用いたHigh-content screening assayの樹立及びHCVエントリー阻害剤探索への応用 (2011)
  • Author(s): 北詰晶子、坂本直哉、奥野友紀子、筬島裕子、中川美奈、柿沼晴、植山真由美、小野塚泉、船岡祐介、渡辺貴子、幾世橋佳、新田沙由梨、村川美也子、萩原正敏、渡辺守
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京(一般口演, O-164)
  • Year and Date: 2011-06-03
• [Presentation] Development of RNA pol-driven adenovirus vector expressing hepatitis C virus replicon (2011)
  • Author(s): Yamagishi Y, Sakamoto N, et al.
  • Organizer: Experimental Biology 2011
  • Place of Presentation: Washington
  • Year and Date: 2011-04-13
• [Presentation] Ezetimibe and interferon synergistically suppress subgenomic replication of hepatitis C virus via the acceleration of IFN-mediated ISG15 conjugation system (2010)
  • Author(s): Masayoshi Yada, Ryoko Yada, Motoyuki Kojima, Kazuto Goto, Naoya Sasamoto, Akihide Masumoto and Makoto Nakamuta
  • Organizer: Asian Pacific Association for the Study of the Liver(APASL) The 7th Single Topic Conference
  • Place of Presentation: Chiba, Japan
  • Year and Date: 2010-12-17
• [Presentation] タイトル:IL28B遺伝子多型と治療抵抗性ウイルス変異からみたC型慢性肝炎の治療抵抗メカニズムの解析 (2010)
  • Author(s): 中川美奈、柿沼晴、坂本直哉
  • Organizer: 第38回日本肝臓学会東部会
  • Place of Presentation: 東京(ワークショップ)
  • Year and Date: 2010-12-02
• [Presentation] ウイルスおよび宿主遺伝子情報に基づくC型慢性肝炎の治療戦略 (2010)
  • Author(s): 中川美奈、柿沼晴、坂本直哉
  • Organizer: 第38回日本肝臓学会東部会
  • Place of Presentation: 東京(パネルディスカッション)
  • Year and Date: 2010-12-02
• [Presentation] C型慢性肝炎のインターフェロン治療効果におけるインターロイキン-6(IL-6)の関与 (2010)
  • Author(s): 中川美奈、坂本直哉、渡辺守
  • Organizer: JDDW2010(第14回日本肝臓学会大会)
  • Place of Presentation: 横浜(パネルディスカッション)
  • Year and Date: 2010-10-14
• [Presentation] HCV増殖を制御する宿主インターフェロン誘導遺伝子GBP-1が介する分子機構 (2010)
  • Author(s): 井津井康浩、坂本直哉、渡辺守
  • Organizer: JDDW2010(第14回日本肝臓学会大会)
  • Place of Presentation: 横浜(ワークショップ)
  • Year and Date: 2010-10-14
• [Presentation] Genotype 2b HCV感染培養系におけるIL-6を介したlnterferon抵抗性発現機構の解析 (2010)
  • Author(s): 須田剛生、坂本直哉、渡辺守
  • Organizer: JDDW2010(第14回日本肝臓学会大会)
  • Place of Presentation: 横浜(ワークショップ)
  • Year and Date: 2010-10-14
• [Presentation] 蛍光タグ付加HCV培養系を用いたウイルスライフサイクルの解析 (2010)
  • Author(s): 山本満千、坂本直哉、中川美奈、柿沼晴、東正新、加藤孝宣、脇田隆字、渡辺守
  • Organizer: JDDW2010
  • Place of Presentation: 横浜(ポスター)
  • Year and Date: 2010-10-13
• [Presentation] 細胞障害性HCV-JFH1subcloneの細胞培養系および感染動物モデルにおける機能解析 (2010)
  • Author(s): 三島果子、坂本直哉、筬島裕子、中川美奈、柿沼晴、東正新、脇田隆字、今村道雄、茶山一彰、渡辺守
  • Organizer: JDDW2010
  • Place of Presentation: 横浜(ポスター)
  • Year and Date: 2010-10-13
• [Presentation] Identification of Novel Small Molecules Inhibitors of Hepatitis C Virus Replication Using a Cell-Based High-Throughput Screen. (2010)
  • Author(s): Akiko Kusano-Kitazume, Naoya Sakamoto, Yuko Sekine-Osajima, Seishin Azuma, Mina Nakagawa, Sei Kakinuma, Yuki Nishimura-Sakurai, Yukiko Okuno, Masatoshi Hagiwara, and Mamoru Watanabe
  • Organizer: 17th International Meeting on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-09-09
• [Presentation] HCV genotype 2a/2b感染クローンを用いたInterferon抵抗性発現機構の解析 (2010)
  • Author(s): 坂本直哉、須田剛生、渡辺守
  • Organizer: 第47回日本消化器免疫学会
  • Place of Presentation: 大津(シンポジウム)
  • Year and Date: 2010-07-07
• [Presentation] 蛍光タグ付加HCV培養系を用いたウイルスライフサイクルの解析.(一般口演) (2010)
  • Author(s): 山本満千、坂本直哉、中川美奈、柿沼晴、東正新、加藤孝宣、脇田隆字、渡辺守
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形
  • Year and Date: 2010-05-27
• [Presentation] HCVレプリコン系を用いた抗ウイルス小分子の大規模スクリーニング.(ポスター) (2010)
  • Author(s): 北詰晶子、坂本直哉、筬島裕子、中川美奈、東正新、柿沼晴、渡辺守
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形
  • Year and Date: 2010-05-27
• [Presentation] ウイルス遺伝子および宿主IL28B遺伝子情報を統合したC型慢性肝炎治療のオーダーメイド化 (2010)
  • Author(s): 坂本直哉、中川美奈、渡辺守
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形(シンポジウム)
  • Year and Date: 2010-05-27
• [Presentation] SR蛋白リン酸化酵素(SRPK)阻害剤によるHCV増殖抑制効果の解析 (2010)
  • Author(s): 唐鎌優子、坂本直哉、小野木博、井津井康浩、中川美奈、柿沼晴、東正新、萩原正敏、渡辺守
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形(ワークショップ)
  • Year and Date: 2010-05-27
• [Presentation] HCV増殖を制御するインターフェロン誘導性蛋白GBP-1が介する分子免疫機構 (2010)
  • Author(s): 井津井康浩、坂本直哉、渡辺守
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟(シンポジウム)
  • Year and Date: 2010-04-22
• [Patent(Industrial Property Rights)] C型肝炎ウイルスの増殖を抑制する医薬組成物 (2011)
  • Inventor(s): 坂本直哉、渡辺守、北詰晶子、萩原正敏、奥野友紀子
  • Industrial Property Rights Holder: 東京医科歯科大学
  • Industrial Property Number: 2011-194082
  • Filing Date: 2011-09-06