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Development of Targeted Adiponectin Delivery System by Using Induced Adipocyte Cell-sheet

Research Project

Project/Area Number 22659251
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionOsaka University

Principal Investigator

SAWA Yoshiki  大阪大学, 医学系・研究科, 教授 (00243220)

Co-Investigator(Kenkyū-buntansha) KURATANI Toru  大阪大学, 医学系・研究科, 准教授 (90448035)
UENO Takayoshi  大阪大学, 医学系・研究科, 講師 (60437316)
SAKAGUCHI Taichi  大阪大学, 医学系・研究科, 寄附講座准教授 (10467574)
SHIRAKAWA Yukitoshi  大阪大学, 医学系・研究科, 助教 (20457013)
YOSHIKAWA Yasushi  大阪大学, 医学部附属病院, 助教 (40570594)
YAMAUCHI Takashi  大阪大学, 医学系・研究科, 助教 (20527999)
SHIMAMURA Kazuo  大阪大学, 医学系・研究科, 寄附講座助教 (10507205)
宮川 繁  大阪大学, 医学系研究科, 助教 (70544237)
Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsDrug delivery system / 劇症型心筋炎 / アディポネクチン / Cell therapy
Research Abstract

We hypothesized that cell sheet-based delivery system of adiponectin might induce immune-tolerance and functional recovery in experimental autoimmune myocarditis(EAM) in rat. Scaffold-free induced adipocyte cell-sheet(iACS) was generated by inducing differentiation of syngeneic cultured adipose tissue-derived stem cells into the adipocytes on the temperature responsive dishes. Supernatants of iACS contained a high level of adiponectin and hepatocyte growth factor(HGF), and reduced proliferation of CD4-positive T cells that were sorted from splenocytes of the EAM rats, in-vitro. This tissue-engineered iACS treatment suppressed intramyocardial T cell activity and inflammation by persistently supplying adipocytokines to the heart, consequently inducing functional recovery in the EAM rat model. The findings support possible translation of this strategy into the clinical arena.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2011 2010

All Journal Article (1 results) Presentation (4 results)

  • [Journal Article] Induced Adipocyte Cell-Sheet Ameliorates Cardiac Dysfunction in a Mouse Myocardial Infarction Model : A Novel Drug Delivery System for Heart Failure2011

    • Author(s)
      Yukiko Imanishi, Shigeru Miyagawa, Norikazu Maeda, Satsuki Fukushima, Satoru Kitagawa-Sakakida, Takashi Daimon, Ayumu Hirata, Tatsuya Shimizu, Teruo Okano, Iichiro Shimomura, Yoshiki Sawa
    • Journal Title

      Circulation

      Volume: 124【suppl 1】

    • Related Report
      2011 Final Research Report
  • [Presentation] A Targeted Delivery of Adipocytokines into the Heart by Transplantation of Induced Adipocyte Cell-Sheet Yields Immune-tolerance and Functional Recovery in Autoimmune-Associated Fulminant Myocarditis in Rat2011

    • Author(s)
      鎌田創吉, 宮川繁, 福嶌五月, 今西悠基子, 齋藤充弘, 前田法一, 澤芳樹
    • Organizer
      アメリカ心臓病学会
    • Place of Presentation
      オーランド
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] A Targeted Delivery of Adipocytokines into the Heart by Transplantation of Induced Adipocyte Cell-sheet Yields Immuno-tolerance and Functional Recovery in Autoimmune-Associated Myocarditis in Rat2011

    • Author(s)
      Sokichi Kamata, Shigeru Miyagawa, Satsuki Fukushima, , Yukiko Imanishi, Atsuhiro Saito, Norikazu Maeda, Yoshiki Sawa
    • Organizer
      アメリカ心臓病学会
    • Place of Presentation
      Orland, USA
    • Year and Date
      2011-11-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 心筋炎ラットに対するアディポネクチン産生細胞シートによる心筋再生効果の検討2011

    • Author(s)
      鎌田創吉
    • Organizer
      日本再生医療学会
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Induced Adipocyte Cell-Sheet Ameliorates Cardiac Dysfunction in a Mouse Myocardial Infarction Model : A Novel Drug Delivery System for Heart Failure2010

    • Author(s)
      Yukiko Imanishi, Shigeru Miyagawa, Norikazu Maeda, Satsuki Fukushima, Satoru Kitagawa-Sakakida, Takashi Daimon, Ayumu Hirata, Tatsuya Shimizu, Teruo Okano, Iichiro Shimomura, Yoshiki Sawa
    • Organizer
      アメリカ心臓病学会
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2010-11-13
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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