| Project/Area Number |
22659297
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Toyama |
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Principal Investigator |
SAITO Shigeru 富山大学, 大学院・医学薬学研究部(医学), 教授 (30175351)
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Hideki 富山大学, 大学病院, 助教 (50401865)
KITAJIMA Isao 富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,950,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | 絨毛膜羊膜炎 / 早産 / 感染 / PCR / Ureaplasma / Mycoplasma / 真菌 / 羊水 / 細菌 / 慢性肺疾患 / 病原微生物 / 迅速診断法 / Universal PCR / 16SrRNA / 産科学 / 検査・診断システム / 子宮内感染 / 病原体検出 / 薬剤感受性試験 |
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| Research Abstract |
We have established a novel method to detect bacterial, Mycoplasma, Ureaplasma and fungi in the amniotic fluid using eukaryote-made Taq polymerase. Using this system, we have evaluated the bacterial infection in amniotic fluid of 86 preterm labor cases. Surprisingly, we have detected double infection with bacterial Ureaplasma in 13 case, Ureaplasma infection cases in 5 cases, bacterial infection cases in 13 cases, and infection rate in preterm labor cases accounted for 37.2%. In double infection cases, amniotic fluid IL-8 was very high （mean: 129.5ng/ml), and frequency of chronic lung disease was also very high （38.5%) compared to those in non-infected cases （5.6%). The duration from the amniocentasis to delivery in double infection cases was significantly shorter than that in no infection cases.
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