Establishment of the dental pulp stem cell using a low molecular weight compound for tooth regeneration

• Project/Area Number: 22659373
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: IWAMOTO Tsutomu 東北大学, 病院, 講師 (90346916)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,320,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 歯髄幹細胞 / GSK3β特異的阻害剤

Research Abstract

We found that BIO, a glycogen synthase kinase-3 (GSK3) inhibitor, induced changes in cell morphology, and promoted the expression of Nanog mRNA, considered the key gene to pluripotency, in mouse dental papilla derived mesenchymal (mDP) cells. When the BIO treated mDP cells were cultured in osteogenic differentiation medium, the expression of osteoblast maker genes and alkaline phosphatase activity were progressively increased.

Research Products

• [Journal Article] Biological roles of gap junction proteins in cartilage and bone development (2013)
  • Author(s): Iwamoto T, Ishikawa M, Ono M, Nakamura T,Fukumoto S, Yamada Y
  • Journal Title: J Oral Biosci Volume: 55(1) Issue: 1 Pages: 29-33
  • DOI: 10.1016/j.job.2012.12.001
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal interaction reduces inhibitory effects of fluoride on proliferation and enamel matrix expression in dental epithelial cells (2012)
  • Author(s): Yamada A, Iwamoto T, Fukumoto E, Arakaki M, Miyamoto R, Sugawara Y, Komatsu H, Nakamura T, Fukumoto S
  • Journal Title: Pediatric Dental Journal Volume: 22 Issue: 1 Pages: 55-63
  • DOI: 10.11411/pdj.22.55
  • NAID: 10030558189
  • ISSN: 0917-2394, 1880-3997
  • Peer Reviewed
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287(13) Issue: 13 Pages: 10590-601
  • DOI: 10.1074/jbc.m111.285874
  • Peer Reviewed
• [Journal Article] Glycosphingolipids regulate ameloblastin expression in dental epithelial cells (2012)
  • Author(s): Kamasaki, et al
  • Journal Title: Journal of Dental Research Volume: 91 Issue: 1 Pages: 78-83
  • DOI: 10.1177/0022034511424408
  • Peer Reviewed
• [Journal Article] Pannexin 3 functions as an ER Ca2+ channel, hemichannel, and gap junction to promote osteoblast differentiation (2011)
  • Author(s): Ishikawa M, Iwamoto T, Nakamura T, Doyle A, Fukumoto S, Yamada Y
  • Journal Title: J Cell Biol Volume: 27;193(7) Issue: 7 Pages: 1257-74
  • DOI: 10.1083/jcb.201101050
  • Peer Reviewed
• [Journal Article] Expressions and Functions of Neurotrophic Factors in Tooth Development (2011)
  • Author(s): Iwamoto T, Yamada A, Arakaki M, Sugawara Y, Ono M, Futaki M, Yoshizaki K, Fukumoto E, Nakamura T, Fukumoto S
  • Journal Title: J Oral Biosci Volume: 53(1) Pages: 113-21
  • NAID: 10027793552
  • URL: http://dx.doi.org/10.2330/joralbiosci.53.13
  • Peer Reviewed
• [Journal Article] Expressions and Functions of Neurotrophic Factors in Tooth Development (2011)
  • Author(s): Iwamoto T, et.al.
  • Journal Title: J Oral Biosci Volume: 53(1) Pages: 113-121
  • NAID: 10027793552
  • Peer Reviewed
• [Journal Article] Pannexin 3 regulates intracellular ATP/cAMP levels and promotes chondrocyte differentiation (2010)
  • Author(s): Iwamoto T, Nakamura T, Doyle A, Ishikawa M, de Vega S, Fukumoto S, Yamada Y
  • Journal Title: J Biol Chem Volume: 285(24) Issue: 24 Pages: 18948-58
  • DOI: 10.1074/jbc.m110.127027
  • Peer Reviewed
• [Journal Article] PDGFs regulate tooth germ proliferation and ameloblastdifferentiation (2010)
  • Author(s): Nun W, Iwamoto T, Sugawara Y, Futaki M, Yoshizaki K, Yamamoto S, Yamada A, Nakamura T, Nonaka K, and Fukumoto S
  • Journal Title: Archives of Oral Biology Volume: 55(6) Pages: 426-34
  • Peer Reviewed
• [Journal Article] Pannexin 3 regulates intracellular ATP/CAMP levels and promotes chondrocyte differentiation. (2010)
  • Author(s): Iwamoto T, et.al.
  • Journal Title: J Biol Chem Volume: 285(24) Pages: 18948-18958
  • Peer Reviewed
• [Journal Article] PDGFs regulate tooth germ proliferation and ameloblast differentiation. (2010)
  • Author(s): Nun W, Iwamoto T, et.al.
  • Journal Title: Archives of Oral Biology Volume: 55(6) Pages: 426-434
  • Peer Reviewed
• [Presentation] 象牙細胞化制御機構の同定とその応用 (2011)
  • Author(s): 岩本勉
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: いわて県民情報交流セン・イナ
  • Year and Date: 2011-11-29
• [Presentation] 象牙芽細胞分化制御機構の同定とその応用 (2011)
  • Author(s): 岩本勉
  • Organizer: 第49回日本小児歯科学会大会
  • Place of Presentation: いわて県民情報交流センター・アイーナ
  • Year and Date: 2011-11-29
• [Presentation] 象牙細胞化における細胞外マトックス(ECM)の役割 (2011)
  • Author(s): 岩本勉
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2011-10-01
• [Presentation] 象牙芽細胞分化における細胞外マトリックス(ECM)の役割 (2011)
  • Author(s): 岩本勉
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2011-10-01
• [Presentation] Pannexin 3 plays a critical role for odontoblast differentiation (2011)
  • Author(s): Tsutomu Iwamoto, Yu Sugawara, Mariko Ono, Aya Yamada, Takashi Nakamura, Satoshi Fukumoto
  • Organizer: American Society For Bone And Mineral Research (ASBMR)2011 Annual Meeting
  • Place of Presentation: San DiegoConvention Center, USA
  • Year and Date: 2011-09-18
• [Presentation] Pannexin 3 plays a critical role for odontoblast differentiation (2011)
  • Author(s): Iwamoto T, et al
  • Organizer: American Society For Bone And Mineral Research (ASBMR) 2011 Annual Meeting
  • Place of Presentation: San Diego Convention Center, USA
  • Year and Date: 2011-09-18
• [Presentation] 歯および軟細胞におけるPannexin3の役割 (2011)
  • Author(s): 岩本勉、福本敏
  • Organizer: 第9回口腔医科学ロンティ学術集会
  • Place of Presentation: 九州大学歯学部本館1階
  • Year and Date: 2011-03-05
• [Presentation] 歯および軟骨細胞におけるPannexin 3の役割 (2011)
  • Author(s): 岩本勉, 他
  • Organizer: 第9回口腔医科学フロンティア学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2011-03-05
• [Presentation] GSK-抑制剤を用いた歯髄細胞の未化法の確立 (2011)
  • Author(s): 福本敏、山田亜矢、岩本勉、中村卓史
  • Organizer: 第3回口腔先端応用医科学研究会
  • Place of Presentation: 東京医科歯科大学歯学部4階特別講堂・演習室
  • Year and Date: 2011-01-22
• [Presentation] GSK-3β抑制剤を用いた歯髄細胞の未分化誘導法の確立 (2011)
  • Author(s): 福本敏、山田亜矢、岩本勉, 他
  • Organizer: 第3回口腔先端応用医科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-01-22
• [Presentation] New gap junctionalprotein pannexin 3 regulates tooth, cartilage and bone development (2011)
  • Author(s): Tsutomu Iwamoto, Mariko Ono, Makiko Arakaki, Takashi Nakamura, Aya Yamada, Satoshi Fukumoto
  • Organizer: Harvard-Forsyth-Tohoku Research Workshop, Belfer Case Study Room
  • Place of Presentation: CGIS South, Harvard University
  • Year and Date: 2011-01-07
• [Presentation] New gap junctional protein pannexin 3 regulates tooth, cartilage and bone development. (2011)
  • Author(s): Iwamoto T, et.al.
  • Organizer: Hazvazd-Forsyth-Tohoku Research Workshop
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-01-07
• [Presentation] Pannexin 3 coordinates odontoblasts proliferation and differentiation by cell-matrix interaction (2010)
  • Author(s): 二木正晴,岩本勉,新垣真紀子,山田亜矢,小野真理子,中村卓史,福本敏
  • Organizer: 第33回日本 子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Pannexin 3 coordinates odontoblasts proliferation and differentiation by cell-matrix interaction. (2010)
  • Author(s): 二木正晴, 岩本勉, 他
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 象牙細胞化におけるギャップ結合子の発現および機能解析 (2010)
  • Author(s): 岩本勉、中村卓史、吉崎恵悟、山田亜矢、福本敏
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: ワホ船堀
  • Year and Date: 2010-09-22
• [Presentation] 象牙芽細胞分化におけるギャップ結合分子の発現および機能解析 (2010)
  • Author(s): 岩本勉, 他
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 船堀
  • Year and Date: 2010-09-22
• [Presentation] 神経栄養因子による歯原性上皮細胞の増殖、分化制御機構の解明 (2010)
  • Author(s): 小野真理子、岩本勉, 他
  • Organizer: 第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 船堀
  • Year and Date: 2010-09-22
• [Presentation] 神経栄養因子による歯原性皮細胞の増殖 (2010)
  • Author(s): 小野真理子、岩本勉、吉崎恵悟、中村卓史、山田亜矢、宮本綾子、福本敏
  • Organizer: 化制御機構の解明、第52回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: ワホ船堀
  • Year and Date: 2010-09-21
• [Presentation] 歯科再生医療実現化を目指した新しい分子機能予測法開発
  • Author(s): 及川知子
  • Organizer: 第31回日本小児歯科学会中四国地方会
  • Place of Presentation: サンポートホール高松