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Analysis of the roles of intracellular ATP during apoptosis by using the ATP imaging technique.

Research Project

Project/Area Number 22687011
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

IMAMURA Hiromi  京都大学, 白眉センター, 准教授 (20422545)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords細胞生物学 / 細胞死 / FRET / イメージング / エネルギー代謝 / ATP放出 / ライブイメージング / 共鳴エネルギー異動 / バイオセンサー / ミトコンドリア
Research Abstract

In this study, we monitored intracellular ATP dynamics during apoptosis by using a newly developed caspase-resistant fluorescent ATP biosensor. We found that intracellular ATP level starts to decrease after the activation of caspase 3, and identified a molecule that is involved in declination of ATP. Moreover, we elucidated that intracellular ATP level regulates plasma membrane blebbing of apoptotic cells.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (45 results)

All 2013 2012 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (32 results) Remarks (1 results)

  • [Journal Article] Distribution and Dynamics of Intracellular ATP Level, and their Regulation2013

    • Author(s)
      今村博臣、安東友美、藤川誠
    • Journal Title

      Seibutsu Butsuri

      Volume: 53 Issue: 1 Pages: 020-023

    • DOI

      10.2142/biophys.53.020

    • NAID

      10031139021

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 細胞内ATPの分布とダイナミクス、その制御2013

    • Author(s)
      今村博臣
    • Journal Title

      生物物理

      Volume: 53 Pages: 20-23

    • NAID

      10031139021

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal correlations between cytosolic and mitochondrial Ca^<2+>signals using a novel red-shifted mitochondrial targeted cameleon2012

    • Author(s)
      Waldeck-Weiermair M, Alam MR, Khan MJ, Deak AT, Vishnu N, Karsten F, Imamura H, Graier WF, Malli R
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 9 Pages: e45917-e45917

    • DOI

      10.1371/journal.pone.0045917

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T2012

    • Author(s)
      Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T
    • Journal Title

      Plant. Cell Physiol

      Volume: 53 Issue: 10 Pages: 1768-1775

    • DOI

      10.1093/pcp/pcs119

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assessing the actual contribution of IF1, an inhibitor of mitochondrial FoF1, to ATP homeostasis, cell growth, mitochondrial morphology and cell viability2012

    • Author(s)
      Fujikawa M, Imamura H, Nakamura J, Yoshida M
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Issue: 22 Pages: 18781-18787

    • DOI

      10.1074/jbc.m112.345793

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization and Measurement of ATP Levels in Living Cells Replicating Hepatitis C Virus Genome RNA2012

    • Author(s)
      安東友美
    • Journal Title

      PLoS Pathogens

      Volume: 8 Issue: 3 Pages: e1002561-e1002561

    • DOI

      10.1371/journal.ppat.1002561

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of ATP sensor protein in Caenorhabditis elegans2012

    • Author(s)
      J. Kishikawa, M. Fujikawa, H. Imamura, K. Yasuda, H. Noji, N. Ishii, S. Mitani, and K. Yokoyama
    • Journal Title

      Microsc. Res. Tech

      Volume: 75(1) Issue: 1 Pages: 15-9

    • DOI

      10.1002/jemt.21103

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ca^<2+>regulation of mitochondrial ATP synthesis visualized at single cell level2011

    • Author(s)
      Nakano M, Imamura H, Nagai T, Noji H
    • Journal Title

      ACS Chem. Biol

      Volume: 6 Issue: 7 Pages: 709-715

    • DOI

      10.1021/cb100313n

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative Glucose and ATP Sensing in Mammalian Cells2011

    • Author(s)
      Liemburg-Apers DC, Imamura H, Forkink M, Nooteboom M, Swarts HG, Brock R, Smeitink JA, Willems PH, Koopman WJ
    • Journal Title

      Pharm. Res

      Volume: 8 Issue: 11 Pages: 2745-2757

    • DOI

      10.1007/s11095-011-0492-8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The leucine zipper EF hand-containing transmembrane protein 1 (LETM1) and uncoupling proteins-2 and 3 (UCP2/3) contribute to two distinct mitochondrial Ca2+uptake pathways2011

    • Author(s)
      Waldeck-Weiermair M, Jean-Quartier C, Rost R, Khan MJ, Vishnu N, Bondarenko AI, Imamura H, Malli R, Graier WF
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 32 Pages: 28444-28455

    • DOI

      10.1074/jbc.m111.244517

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新規FRETプローブを用いた生細胞内ATP濃度イメニジング法2010

    • Author(s)
      今村博臣
    • Journal Title

      実験医学

      Volume: 82 Pages: 1056-1060

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 蛍光イメージングによる細胞内および細胞外ATPの可視化2010

    • Author(s)
      今村博臣
    • Journal Title

      生化学

      Volume: 82 Pages: 425-437

    • NAID

      10027708738

    • Related Report
      2010 Annual Research Report
  • [Presentation] Imaging of intracellular ATP dynamics in single apoptotic cells2013

    • Author(s)
      Sakamoto S, Kakizuka A, Imamura H
    • Organizer
      11thInternational Student Seminar
    • Place of Presentation
      Kyoto University, Japan
    • Year and Date
      2013-03-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Imaging of intracellular ATP dynamics in single apoptotic cells2013

    • Author(s)
      坂本修一朗
    • Organizer
      11th International Student Seminar
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] アポトーシスにおける細胞内 ATP の挙動の解析2012

    • Author(s)
      坂本修一朗、野地博行、垣塚彰、 今村博臣
    • Organizer
      日本生体エネルギー研究会 第38回討論会
    • Place of Presentation
      岡山大学(岡山)
    • Year and Date
      2012-12-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Pannexin1 は細胞内 ATP レベルを制御することでアポトーシスの進行に重要な役割を果たす2012

    • Author(s)
      坂本修一朗、野地博行、垣塚彰、 今村博臣
    • Organizer
      日本生体エネルギー研究会第37回討論会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-12-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 飢餓条件下における VCP タンパク質の機能解析2012

    • Author(s)
      小池雅昭、 今村博臣、垣塚彰
    • Organizer
      日本生体エネルギー研究会 第37回討論会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-12-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Fluorescent ATP biosensor optimized for imaging of cells from non-vertebrate model organisms2012

    • Author(s)
      Imamura H, Tsuyama T, Uemura T, Kakizuka A
    • Organizer
      International Symposia "New Frontiers of Metabolism Research in Biomedical Sciences"
    • Place of Presentation
      University of Tokyo, Japan
    • Year and Date
      2012-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Genetically-encoded ATP biosensor for low temperatures2012

    • Author(s)
      Imamura H, Tsuyama T, Kishikawa J, Noji H, Kakizuka A, Yokoyama K, Uemura T
    • Organizer
      17thEuropean Bioenergetic Conference
    • Place of Presentation
      University of Freiburg, Germany
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Simultaneous ATP and Ca2+ imaging of a single insulin-secreting islet eell2012

    • Author(s)
      Tanaka T., et al.
    • Organizer
      The 10th NTU-Japan International mini-symposium on Molecular and Cell Biology
    • Place of Presentation
      国立台湾大学
    • Year and Date
      2012-01-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] アポトーシスにおける細胞内ATPの挙動の解析2012

    • Author(s)
      坂本修一朗
    • Organizer
      日本生体エネルギー研究会 第38回討論会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] Fluorescent ATP biosensor optimized for imaging of cells from non-vertebrate model organisms2012

    • Author(s)
      今村博臣
    • Organizer
      International Symposia “New Frontiers of Metabolism Research in Biomedical Sciences”
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetically-encoded ATP biosensor for low temperatures2012

    • Author(s)
      今村博臣
    • Organizer
      European Bioenergetic Conference
    • Place of Presentation
      フライブルク
    • Related Report
      2012 Annual Research Report
  • [Presentation] Distribution and dynamics of ATP level inside living cells2011

    • Author(s)
      Imamura H
    • Organizer
      1stNIBB-Princeton Symposium
    • Place of Presentation
      Okazaki Conference Center, Japan
    • Year and Date
      2011-11-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Distribution and dynamics of ATP level inside living cells2011

    • Author(s)
      Hiromi Imamura
    • Organizer
      1st NIBB-Princeton Symposium
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2011-11-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミトコンドリア ATP 濃度は細胞質 ATP 濃度とは半独立に制御されている2011

    • Author(s)
      今村博臣、野地博行、垣塚彰
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館(京都)
    • Year and Date
      2011-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] ATP 結合タンパク質を利用した細胞内 ATP の可視化2011

    • Author(s)
      今村博臣
    • Organizer
      酵素工学研究会第65回講演会
    • Place of Presentation
      京都テルサ(京都)
    • Year and Date
      2011-04-22
    • Related Report
      2012 Final Research Report
  • [Presentation] ATP結合タンパク質を利用した細胞内ATPの可視化2011

    • Author(s)
      今村博臣
    • Organizer
      酵素工学研究会第65回公演会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2011-04-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 飢餓条件下におけるVCPタンパク質の機能解析2011

    • Author(s)
      小池雅昭、今村博臣、垣塚彰
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミトコンドリアATP濃度は細胞質ATP濃度とは半独立に制御されている2011

    • Author(s)
      今村博臣, 他
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] 飢餓条件下におけるVCPタンパク質の機能解析2011

    • Author(s)
      小池雅昭、今村博臣、垣塚彰
    • Organizer
      第37回日本生体エネルギー研究会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Pannexin1は細胞内ATPレベルを制御することでアポトーシスの進行に重要な役割を果たす2011

    • Author(s)
      坂本修一朗, 他
    • Organizer
      第37回日本生体エネルギー研究会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATPバイオセンサーを用いた膵島β細胞のライブイメージング2011

    • Author(s)
      田中喬, 他
    • Organizer
      第37回日本生体エネルギー研究会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内ATP とカルシウムの同時イメージング2010

    • Author(s)
      中野雅裕、今村博臣、野地博行
    • Organizer
      第2回光塾
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2010-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内ATPとカルシウムの同時イメージング2010

    • Author(s)
      中野雅裕、今村博臣、野地博行
    • Organizer
      第2回光塾
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 赤色シフトした蛍光ATPバイオセンサーを用いた単一細胞内ATPおよびカルシウムの同時イメージング2010

    • Author(s)
      中野雅裕、今村博臣、野地博行
    • Organizer
      第36回日本生体エネルギー研究会
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Red-shifted genetially-encoded fluorescent ATP indicators enabled simultaneous imaging of ATP and Ca2+ inside single living cells2010

    • Author(s)
      中野雅裕、今村博臣、野地博行
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学(宮城)
    • Year and Date
      2010-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Red-shifted genetically-encoded fluorescent ATP indicators enabled simultaneous imaging of ATP and Ca2+ inside single living cells2010

    • Author(s)
      中野雅裕、今村博臣、野地博行
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Simultaneous ratiometric imaging of ATP and Ca2+ concentrations inside single living cells2010

    • Author(s)
      Imamura H, Nakano M, Noji H
    • Organizer
      16thEuropean Bioenergetics Conference
    • Place of Presentation
      University of Warsaw, Poland
    • Year and Date
      2010-07-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Simultaneous ratiometric imaging of ATP and Ca2+ concentrations inside single living cells2010

    • Author(s)
      H.Imamura, M.Nakano, H.Noji
    • Organizer
      16th Europe Bioenergetics Conference
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2010-07-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] ATP concentration change in Caenorhabditis elegans2010

    • Author(s)
      Kishikawa J, Fujikawa H, Imamura H, Yasuda K, Ishii N, Mitani S, Noji H, Yokoyama K
    • Organizer
      16thEuropean Bioenergetics Conference
    • Place of Presentation
      University of Warsaw, Poland
    • Year and Date
      2010-07-19
    • Related Report
      2012 Final Research Report
  • [Presentation] ATP concentration change in Caenorhabditis elegans2010

    • Author(s)
      J.Kishikawa, M.Fujikawa, H.Imamura, K.Yasuda, N.Ishii, S.Mitani, H.Noji, K.Yokoyama
    • Organizer
      16th Europe Bioenergetics Conference
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2010-07-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Imaging of intracellular ATP: Looking into cells through ATP2010

    • Author(s)
      Imamura H
    • Organizer
      NIG Symposium 2010 "New Frontiers in Genetics"
    • Place of Presentation
      National Institute of Genetics, Japan
    • Year and Date
      2010-04-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Imaging of intracellular ATP : Looking into cells through ATP2010

    • Author(s)
      今村博臣
    • Organizer
      NIG Symposium 2010 "New Frontiers in Genetics"
    • Place of Presentation
      国立遺伝学研究所
    • Year and Date
      2010-04-09
    • Related Report
      2010 Annual Research Report
  • [Remarks] Imamura group HP

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/imamura/Site/Top.html

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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