Analysis of the roles of intracellular ATP during apoptosis by using the ATP imaging technique.

• Project/Area Number: 22687011
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: IMAMURA Hiromi 京都大学, 白眉センター, 准教授 (20422545)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000); Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000); Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 細胞生物学 / 細胞死 / FRET / イメージング / エネルギー代謝 / ATP放出 / ライブイメージング / 共鳴エネルギー異動 / バイオセンサー / ミトコンドリア

Research Abstract

In this study, we monitored intracellular ATP dynamics during apoptosis by using a newly developed caspase-resistant fluorescent ATP biosensor. We found that intracellular ATP level starts to decrease after the activation of caspase 3, and identified a molecule that is involved in declination of ATP. Moreover, we elucidated that intracellular ATP level regulates plasma membrane blebbing of apoptotic cells.

Research Products

• [Journal Article] Distribution and Dynamics of Intracellular ATP Level, and their Regulation (2013)
  • Author(s): 今村博臣、安東友美、藤川誠
  • Journal Title: Seibutsu Butsuri Volume: 53 Issue: 1 Pages: 020-023
  • DOI: 10.2142/biophys.53.020
  • NAID: 10031139021
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] 細胞内ATPの分布とダイナミクス、その制御 (2013)
  • Author(s): 今村博臣
  • Journal Title: 生物物理 Volume: 53 Pages: 20-23
  • NAID: 10031139021
  • Peer Reviewed
• [Journal Article] Spatiotemporal correlations between cytosolic and mitochondrial Ca^<2+>signals using a novel red-shifted mitochondrial targeted cameleon (2012)
  • Author(s): Waldeck-Weiermair M, Alam MR, Khan MJ, Deak AT, Vishnu N, Karsten F, Imamura H, Graier WF, Malli R
  • Journal Title: PLoS ONE Volume: 7 Issue: 9 Pages: e45917-e45917
  • DOI: 10.1371/journal.pone.0045917
  • Peer Reviewed
• [Journal Article] Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T (2012)
  • Author(s): Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T
  • Journal Title: Plant. Cell Physiol Volume: 53 Issue: 10 Pages: 1768-1775
  • DOI: 10.1093/pcp/pcs119
  • Peer Reviewed
• [Journal Article] Assessing the actual contribution of IF1, an inhibitor of mitochondrial FoF1, to ATP homeostasis, cell growth, mitochondrial morphology and cell viability (2012)
  • Author(s): Fujikawa M, Imamura H, Nakamura J, Yoshida M
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 22 Pages: 18781-18787
  • DOI: 10.1074/jbc.m112.345793
  • Peer Reviewed
• [Journal Article] Visualization and Measurement of ATP Levels in Living Cells Replicating Hepatitis C Virus Genome RNA (2012)
  • Author(s): 安東友美
  • Journal Title: PLoS Pathogens Volume: 8 Issue: 3 Pages: e1002561-e1002561
  • DOI: 10.1371/journal.ppat.1002561
  • Peer Reviewed
• [Journal Article] Expression of ATP sensor protein in Caenorhabditis elegans (2012)
  • Author(s): J. Kishikawa, M. Fujikawa, H. Imamura, K. Yasuda, H. Noji, N. Ishii, S. Mitani, and K. Yokoyama
  • Journal Title: Microsc. Res. Tech Volume: 75(1) Issue: 1 Pages: 15-9
  • DOI: 10.1002/jemt.21103
  • Peer Reviewed
• [Journal Article] Ca^<2+>regulation of mitochondrial ATP synthesis visualized at single cell level (2011)
  • Author(s): Nakano M, Imamura H, Nagai T, Noji H
  • Journal Title: ACS Chem. Biol Volume: 6 Issue: 7 Pages: 709-715
  • DOI: 10.1021/cb100313n
  • Peer Reviewed
• [Journal Article] Quantitative Glucose and ATP Sensing in Mammalian Cells (2011)
  • Author(s): Liemburg-Apers DC, Imamura H, Forkink M, Nooteboom M, Swarts HG, Brock R, Smeitink JA, Willems PH, Koopman WJ
  • Journal Title: Pharm. Res Volume: 8 Issue: 11 Pages: 2745-2757
  • DOI: 10.1007/s11095-011-0492-8
  • Peer Reviewed
• [Journal Article] The leucine zipper EF hand-containing transmembrane protein 1 (LETM1) and uncoupling proteins-2 and 3 (UCP2/3) contribute to two distinct mitochondrial Ca2+uptake pathways (2011)
  • Author(s): Waldeck-Weiermair M, Jean-Quartier C, Rost R, Khan MJ, Vishnu N, Bondarenko AI, Imamura H, Malli R, Graier WF
  • Journal Title: J Biol Chem Volume: 286 Issue: 32 Pages: 28444-28455
  • DOI: 10.1074/jbc.m111.244517
  • Peer Reviewed
• [Journal Article] 新規FRETプローブを用いた生細胞内ATP濃度イメニジング法 (2010)
  • Author(s): 今村博臣
  • Journal Title: 実験医学 Volume: 82 Pages: 1056-1060
• [Journal Article] 蛍光イメージングによる細胞内および細胞外ATPの可視化 (2010)
  • Author(s): 今村博臣
  • Journal Title: 生化学 Volume: 82 Pages: 425-437
  • NAID: 10027708738
• [Presentation] Imaging of intracellular ATP dynamics in single apoptotic cells (2013)
  • Author(s): Sakamoto S, Kakizuka A, Imamura H
  • Organizer: 11thInternational Student Seminar
  • Place of Presentation: Kyoto University, Japan
  • Year and Date: 2013-03-10
• [Presentation] Imaging of intracellular ATP dynamics in single apoptotic cells (2013)
  • Author(s): 坂本修一朗
  • Organizer: 11th International Student Seminar
  • Place of Presentation: 京都
• [Presentation] アポトーシスにおける細胞内 ATP の挙動の解析 (2012)
  • Author(s): 坂本修一朗、野地博行、垣塚彰、 今村博臣
  • Organizer: 日本生体エネルギー研究会 第38回討論会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 2012-12-24
• [Presentation] Pannexin1 は細胞内 ATP レベルを制御することでアポトーシスの進行に重要な役割を果たす (2012)
  • Author(s): 坂本修一朗、野地博行、垣塚彰、 今村博臣
  • Organizer: 日本生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2012-12-21
• [Presentation] 飢餓条件下における VCP タンパク質の機能解析 (2012)
  • Author(s): 小池雅昭、 今村博臣、垣塚彰
  • Organizer: 日本生体エネルギー研究会 第37回討論会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2012-12-21
• [Presentation] Fluorescent ATP biosensor optimized for imaging of cells from non-vertebrate model organisms (2012)
  • Author(s): Imamura H, Tsuyama T, Uemura T, Kakizuka A
  • Organizer: International Symposia "New Frontiers of Metabolism Research in Biomedical Sciences"
  • Place of Presentation: University of Tokyo, Japan
  • Year and Date: 2012-09-28
• [Presentation] Genetically-encoded ATP biosensor for low temperatures (2012)
  • Author(s): Imamura H, Tsuyama T, Kishikawa J, Noji H, Kakizuka A, Yokoyama K, Uemura T
  • Organizer: 17thEuropean Bioenergetic Conference
  • Place of Presentation: University of Freiburg, Germany
  • Year and Date: 2012-09-19
• [Presentation] Simultaneous ATP and Ca2+ imaging of a single insulin-secreting islet eell (2012)
  • Author(s): Tanaka T., et al.
  • Organizer: The 10th NTU-Japan International mini-symposium on Molecular and Cell Biology
  • Place of Presentation: 国立台湾大学
  • Year and Date: 2012-01-08
• [Presentation] アポトーシスにおける細胞内ATPの挙動の解析 (2012)
  • Author(s): 坂本修一朗
  • Organizer: 日本生体エネルギー研究会 第38回討論会
  • Place of Presentation: 岡山
• [Presentation] Fluorescent ATP biosensor optimized for imaging of cells from non-vertebrate model organisms (2012)
  • Author(s): 今村博臣
  • Organizer: International Symposia “New Frontiers of Metabolism Research in Biomedical Sciences”
  • Place of Presentation: 東京
• [Presentation] Genetically-encoded ATP biosensor for low temperatures (2012)
  • Author(s): 今村博臣
  • Organizer: European Bioenergetic Conference
  • Place of Presentation: フライブルク
• [Presentation] Distribution and dynamics of ATP level inside living cells (2011)
  • Author(s): Imamura H
  • Organizer: 1stNIBB-Princeton Symposium
  • Place of Presentation: Okazaki Conference Center, Japan
  • Year and Date: 2011-11-01
• [Presentation] Distribution and dynamics of ATP level inside living cells (2011)
  • Author(s): Hiromi Imamura
  • Organizer: 1st NIBB-Princeton Symposium
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2011-11-01
• [Presentation] ミトコンドリア ATP 濃度は細胞質 ATP 濃度とは半独立に制御されている (2011)
  • Author(s): 今村博臣、野地博行、垣塚彰
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Presentation] ATP 結合タンパク質を利用した細胞内 ATP の可視化 (2011)
  • Author(s): 今村博臣
  • Organizer: 酵素工学研究会第65回講演会
  • Place of Presentation: 京都テルサ(京都)
  • Year and Date: 2011-04-22
• [Presentation] ATP結合タンパク質を利用した細胞内ATPの可視化 (2011)
  • Author(s): 今村博臣
  • Organizer: 酵素工学研究会第65回公演会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2011-04-22
• [Presentation] 飢餓条件下におけるVCPタンパク質の機能解析 (2011)
  • Author(s): 小池雅昭、今村博臣、垣塚彰
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館
• [Presentation] ミトコンドリアATP濃度は細胞質ATP濃度とは半独立に制御されている (2011)
  • Author(s): 今村博臣, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館
• [Presentation] 飢餓条件下におけるVCPタンパク質の機能解析 (2011)
  • Author(s): 小池雅昭、今村博臣、垣塚彰
  • Organizer: 第37回日本生体エネルギー研究会
  • Place of Presentation: 京都産業大学
• [Presentation] Pannexin1は細胞内ATPレベルを制御することでアポトーシスの進行に重要な役割を果たす (2011)
  • Author(s): 坂本修一朗, 他
  • Organizer: 第37回日本生体エネルギー研究会
  • Place of Presentation: 京都産業大学
• [Presentation] ATPバイオセンサーを用いた膵島β細胞のライブイメージング (2011)
  • Author(s): 田中喬, 他
  • Organizer: 第37回日本生体エネルギー研究会
  • Place of Presentation: 京都産業大学
• [Presentation] 細胞内ATP とカルシウムの同時イメージング (2010)
  • Author(s): 中野雅裕、今村博臣、野地博行
  • Organizer: 第2回光塾
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2010-12-11
• [Presentation] 細胞内ATPとカルシウムの同時イメージング (2010)
  • Author(s): 中野雅裕、今村博臣、野地博行
  • Organizer: 第2回光塾
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-12-11
• [Presentation] 赤色シフトした蛍光ATPバイオセンサーを用いた単一細胞内ATPおよびカルシウムの同時イメージング (2010)
  • Author(s): 中野雅裕、今村博臣、野地博行
  • Organizer: 第36回日本生体エネルギー研究会
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-11-20
• [Presentation] Red-shifted genetially-encoded fluorescent ATP indicators enabled simultaneous imaging of ATP and Ca2+ inside single living cells (2010)
  • Author(s): 中野雅裕、今村博臣、野地博行
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(宮城)
  • Year and Date: 2010-09-20
• [Presentation] Red-shifted genetically-encoded fluorescent ATP indicators enabled simultaneous imaging of ATP and Ca2+ inside single living cells (2010)
  • Author(s): 中野雅裕、今村博臣、野地博行
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] Simultaneous ratiometric imaging of ATP and Ca2+ concentrations inside single living cells (2010)
  • Author(s): Imamura H, Nakano M, Noji H
  • Organizer: 16thEuropean Bioenergetics Conference
  • Place of Presentation: University of Warsaw, Poland
  • Year and Date: 2010-07-21
• [Presentation] Simultaneous ratiometric imaging of ATP and Ca2+ concentrations inside single living cells (2010)
  • Author(s): H.Imamura, M.Nakano, H.Noji
  • Organizer: 16th Europe Bioenergetics Conference
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2010-07-21
• [Presentation] ATP concentration change in Caenorhabditis elegans (2010)
  • Author(s): Kishikawa J, Fujikawa H, Imamura H, Yasuda K, Ishii N, Mitani S, Noji H, Yokoyama K
  • Organizer: 16thEuropean Bioenergetics Conference
  • Place of Presentation: University of Warsaw, Poland
  • Year and Date: 2010-07-19
• [Presentation] ATP concentration change in Caenorhabditis elegans (2010)
  • Author(s): J.Kishikawa, M.Fujikawa, H.Imamura, K.Yasuda, N.Ishii, S.Mitani, H.Noji, K.Yokoyama
  • Organizer: 16th Europe Bioenergetics Conference
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2010-07-19
• [Presentation] Imaging of intracellular ATP: Looking into cells through ATP (2010)
  • Author(s): Imamura H
  • Organizer: NIG Symposium 2010 "New Frontiers in Genetics"
  • Place of Presentation: National Institute of Genetics, Japan
  • Year and Date: 2010-04-09
• [Presentation] Imaging of intracellular ATP : Looking into cells through ATP (2010)
  • Author(s): 今村博臣
  • Organizer: NIG Symposium 2010 "New Frontiers in Genetics"
  • Place of Presentation: 国立遺伝学研究所
  • Year and Date: 2010-04-09
• [Remarks] Imamura group HP
  • URL: http://www.lif.kyoto-u.ac.jp/labs/imamura/Site/Top.html