Analysis of the roles of intracellular ATP during apoptosis by using the ATP imaging technique.
| Project/Area Number |
22687011
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
IMAMURA Hiromi 京都大学, 白眉センター, 准教授 (20422545)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
|
|---|
| Keywords | 細胞生物学 / 細胞死 / FRET / イメージング / エネルギー代謝 / ATP放出 / ライブイメージング / 共鳴エネルギー異動 / バイオセンサー / ミトコンドリア |
|---|
| Research Abstract |
In this study, we monitored intracellular ATP dynamics during apoptosis by using a newly developed caspase-resistant fluorescent ATP biosensor. We found that intracellular ATP level starts to decrease after the activation of caspase 3, and identified a molecule that is involved in declination of ATP. Moreover, we elucidated that intracellular ATP level regulates plasma membrane blebbing of apoptotic cells.
|
Report
(4 results)
Research Products
(45 results)
