| Budget Amount *help |
¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2012: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Research Abstract |
The mechanism(s) by which untimely meiosis is prevented in mitotic cells is not understood fully. Using the fission yeast Schizosaccharomyces pombe, we studied mRNA degradation-based suppression of meiosis.
We isolated Red1, which forms nuclear foci, and found that 1) Red1 promotes selective degradation of meiotic mRNAs in vegetative cells; 2) Red1 forms a complex with two novel proteins Red2 and Red3, both of which are essential for meiotic mRNA elimination; 3) Red5 facilitates meiotic mRNA decay after the Red1 complex adds poly(A) tails to target mRNAs; and 4) Rhn1, which is homologous to a transcription termination factor, suppresses meiotic mRNAs in growing cells, and Cids-2, an Rhn1 homolog in C. elegans, also suppress germ-specific transcripts in somatic cells. These results suggest that meiotic mRNA suppression is a common mechanism by which meiosis is inhibited in eukaryotes.
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