Comprehensive analyses of DNA Topoisomerase I and relating DNA repair
| Project/Area Number |
22710061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
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| Research Collaborator |
HONMA Masamitsu 国立医薬品食品衛生研究所, 変異遺伝部, 室長 (30250179)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 修復 / ゲノム / プロテオーム / DNA修復 / 突然変異 / 放射線 |
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| Research Abstract |
DNA topoisomerase I(Top1) is an essential enzyme involved in resolving the torsional stress associated with DNA replication and transcription. Top1 have a deleterious effect on cells in these processes. In the event, when the final re-ligation step of the reaction cycle is prevented, the covalent topoisomerase I. DNA intermediate becomes a toxic DNA lesion, called topoisomerase I-DNA covalent complex(Top1-cc). These lesions must be repaired. The molecular mechanisms of repair of Top1-cc seem to be complicated with DNA replication, transcription, recombination and protein degradation. However, detailed mechanisms are not clear. Here we purified and analyzed Top1 protein complexes to know molecular mechanisms of repair of Top1-cc, Top1 itself and interaction proteins.
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Report
(3 results)
Research Products
(46 results)
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[Journal Article] these authors contributed equally to this work. Mutations in UVSSA cause UV-sensitive syndrome and destabilize ERCC6 in transcription-coupled DNA repair2012
Author(s)
Xue Zhang, Katsuyoshi Horibata, Masafumi Saijo, Chie Ishigami, Akiko Ukai, Shin-ichiro Kanno, Hidetoshi Tahara, Edward G Neilan, Masamitsu Honma, Takehiko Nohmi, Akira Yasui and Kiyoji Tanaka
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Journal Title
Nature Genetics
Volume: 44(5)
Pages: 593-597
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Peer Reviewed
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[Presentation] Molecular cloning of the gene for UV-sensitive syndrome with deficiencies in transcription-coupled DNA repair2011
Author(s)
Xue Zhang, Katsuyoshi Horibata, Masafumi Saijo, Chie Ishigami, Akiko Ukai, Shinichiro Kanno, Edward. G Neilan, Hidetoshi Tahara, Masamitsu Honma, Takehiko Nohmi, Akira Yasui and Kiyoji Tanaka
Organizer
第34回日本分子生物学会年会
Year and Date
2011-12-15
Related Report
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[Presentation] UVSSA and USP7 cooperate to stabilize CSB in transcription-coupled DNA repair2011
Author(s)
Masafumi Saijo, Xue Zhang, Katsuyoshi Horibata, Chie Ishigami, Akiko Ukai, Shin-ichiro Kanno, Hidetoshi Tahara, Edward G. Neilan, Masamitsu Honma, Takehiko Nohmi, Akira Yasui and Kiyoji Tanaka
Organizer
第34回日本分子生物学会年会
Year and Date
2011-12-15
Related Report
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[Presentation] 新規in vivo遺伝子突然変異評価系(Pig-aアッセイ)の検討:骨髄エリスロイド及び末梢血網状赤血球を用いるPig-aアッセイの開発2011
Author(s)
木本崇文,千藏さつき,鈴木久美子,小林小梅,板野泰弘, Vasily N. Dobrovolsky, Robert H. Heflich,堀端克良,本間正充,三浦大志郎,笠原義典
Organizer
第38回日本トキシコロジー学会学術年会
Year and Date
2011-07-11
Related Report
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[Presentation] Molecular cloning of the gene for UV-Sensitive Syndrome with deficiencies in Transcription-coupled DNA repair2011
Author(s)
Xue Zhang, Katsuyoshi Horibata, Masafumi Saijo, Chie Ishigami, Akiko Ukai, Shinichiro Kanno, Edward G. Neilan, Hidetoshi Tahara, Masamitsu Honma, Takehiko Nohmi, Akira Yasui and Kiyoji Tanaka
Organizer
Conference : Response to DNA damage : from molecular mechanism to human disease
Place of Presentation
Egmond aan Zee, Netherlands
Year and Date
2011-04-04
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