Evidences of a novel splicing mechanism in DMD gene

• Project/Area Number: 22710192
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical genome science
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: SUZUKI Hitoshi 北陸先端科学技術大学院大学, ナノマテリアルテクノロジーセンター, 助教 (00447690)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: RNA / スプライシング / 疾患関連遺伝子 / エキソンスキップ治療

Research Abstract

Lariat intron is a by-product generated by pre-mRNA splicing. In this study, I investigated a possibility that an ends-connecting intron could be produced in vivo. The results suggested that the ends-connecting introns were widely detected. There was likely to be no tissue dependence or intron dependence. Besides, it was suggested that RNase R-resistant RNA contained not only intron RNAs, but also many unknown RNAs located in the first exon regions.

Research Products

• [Journal Article] Re-splicing of mature mRNA in cancer cells promotes activation of distant weak alternative splice sites (2012)
  • Author(s): Kameyama, T., Suzuki, H., and Mayeda, A.
  • Journal Title: Nucleic Acids Res Volume: (in press)
• [Journal Article] Alternative splicing produces structural and functional changes in CUGBP2 (2012)
  • Author(s): Suzuki, H., Takeuchi, M., Sugiyama, A., Alam, A. H. M. K., Vu, T. L., Sekiyama, Y., Dam, C. H., Ohki, S., and Tsukahara, T.
  • Journal Title: BMC Biochem Volume: (in press)
• [Journal Article] Functional gene expression analysis of tissue-specific is forms of Mef2c (2012)
  • Author(s): Sekiyama, Y., Suzuki, H., and Tsukahara, T.
  • Journal Title: Cell. Mol Volume: 32 Pages: 129-139
• [Journal Article] Alternative Splicing Produces Structural and Functional Changes in CUGBP2 (2012)
  • Author(s): 鈴木 仁
  • Journal Title: BMC Biochemistry Volume: 13 Issue: 1 Pages: 6-6
  • DOI: 10.1186/1471-2091-13-6
  • Peer Reviewed
• [Journal Article] Functional gene expression analysis of tissue-specific isoforms of Mef2c (2012)
  • Author(s): 関山佳治
  • Journal Title: Cellular and Molecular Neurobiology Volume: 32 Issue: 1 Pages: 129-139
  • DOI: 10.1007/s10571-011-9743-9
  • Peer Reviewed
• [Journal Article] Comprehensive Analysis of Alternative Splicings and Functionality in Neuronal Differentiation of P19 cells (2011)
  • Author(s): Suzuki, H., Osaki, K., Sano, K., Alam, A. H. M. K., Nakamura, Y., Ishigaki, Y., Kawahara, K., and Tsukahara, T.
  • Journal Title: PLoS One Volume: 6
• [Journal Article] Comprehensive Analysis of Alternative Splicings and Functionality in Neuronal Differentiation of P19 cells (2011)
  • Author(s): 鈴木仁
  • Journal Title: PLoS One Volume: 6 Pages: 16880-16880
  • Peer Reviewed
• [Journal Article] Retinoic acid treatment and cell aggregation independently regulate alternative splicing in P19 cells during neural differentiation (2010)
  • Author(s): Alam, A. H. M. K., Suzuki, H., and Tsukahara, T.
  • Journal Title: Cell. Biol. Int Volume: 34 Pages: 631-643
• [Journal Article] Retinoic acid treatment and cell aggregation independently regulate alternative splicing in P19 cells during neural differentiation (2010)
  • Author(s): AHMK Alam
  • Journal Title: Cell Biol Int. Volume: 34 Pages: 631-643
  • Peer Reviewed
• [Presentation] P19細胞の神経分化における選択的スプライシングの網羅的解析と経路網探索 (2010)
  • Author(s): 鈴木仁
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-28
• [Presentation] P19細胞の神経分化における選択的スプライシングの網羅的解析と経路網探索 (2010)
  • Author(s): 鈴木仁
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 一橋記念講堂(東京)
  • Year and Date: 2010-07-28
• [Remarks]