Screening of new promoting factor for active recombinant-protein from marine metagenomic library
| Project/Area Number |
22710204
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
TERAHARA Takeshi 東京海洋大学, 大学院・海洋科学技術研究科, 助教 (70547059)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 環境ゲノム / メタゲノム / 遺伝子資源 |
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| Research Abstract |
Escherichia coli expression system has generally been used for producing recombinant proteins, whereas it often results in insoluble and/or non-functional proteins. In this study, approximately 2, 000 E. coli strains of marine metagenomic library were screened for new promoting factor for active recombinant-proteins. Three positive clones, which promote growth of E. coli at low temperature, were obtained. In addition, these clones contained putative new factors. The results indicate that these clones may be useful for the construction of new E. coli expression system at low temperature in order to produce active recombinant-proteins.
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Report
(3 results)
Research Products
(2 results)
