Development of Bioluminescent Probe for Real-Time Imaging ofTernary Complex Formation in Living Cells

• Project/Area Number: 22750067
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KANNO Akira 東京大学, 大学院・理学系研究科, 助教 (60466795)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分析化学 / 生体分子 / レポータータンパク質 / タンパク質再構成法 / タンパク質間相互作用

Research Abstract

Various chemical processes and signal transductions are mainly executed by protein-protein interaction. Therefore, it is important to monitor protein-protein interactions in living cells non-invasively. In this study, I developed a bioluminescent probe for highly sensitive real-timesensing of the formation of protein ternary complexes in living mammalian cells.

Research Products

• [Journal Article] Detection of Protein-Protein Interactions in Bacteria by GFP-Fragment Reconstitution (2011)
  • Author(s): A. Kanno, T. Ozawa, Y. Umezawa
  • Journal Title: Methods Mol. Biol. Volume: 705 Pages: 251-258
• [Journal Article] タンパク質核内移行の可視化 (2011)
  • Author(s): 菅野憲,小澤岳昌
  • Journal Title: 生体の科学 Volume: 62 Pages: 494-495
• [Journal Article] Detection of Protein-Protein Interactions in Bacteria by GFP-Fragment Reconstitution (2011)
  • Author(s): A.Kanno, T.Ozawa, Y.Umezawa
  • Journal Title: Methods Mol.Biol. Volume: 705 Pages: 251-258
• [Journal Article] 生体内のカスパーゼ活性を可視化検出する環状ルシフェラーゼの開発 (2010)
  • Author(s): 菅野憲,梅澤喜夫,小澤岳昌
  • Journal Title: Chemical Biology(日本ケミカルバイオロジー学会機関誌) Volume: 3 Pages: 2-6
• [Presentation] Genetically Encoded Bioluminescent Indicators for Real-Time Dual Imaging of Protease Activities in Living Cells (2012)
  • Author(s): Akria Kanno, Katsuhito Nakazono, Yoshio Umezawa, Takeaki Ozawa
  • Organizer: IUPAC International Congress on Analytical Sciences 2011 (ICAS2011)
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2012-05-24
• [Presentation] Genetically Encoded Split Protein Fragments to Illuminate Biological Functions in Living Subjects (2012)
  • Author(s): Akria Kanno, Takeaki Ozawa
  • Organizer: 5th International Symposium on Nanomedicine (ISNM2012)
  • Place of Presentation: 名古屋大学(愛知県)(招待講演)
  • Year and Date: 2012-03-17
• [Presentation] エストロゲンを高感度にリアルタイム検出する発光インジケーターの開発 (2012)
  • Author(s): 菅野憲,竹之内修,高倉栄男,小澤岳昌
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島
• [Presentation] Genetically EncodedSplit Protein Fragments to Illuminate Biological Functions in Living Subjects (2012)
  • Author(s): A. Kanno, T. Ozawa
  • Organizer: 5th International Symposium on Nanomedicine
  • Place of Presentation: Nagoya
• [Presentation] Genetically Encoded Split Protein Fragments to Illuminate Biological Functions in Living Subjects (2012)
  • Author(s): A. Kanno
  • Organizer: 5th International Symposium on Nanomedicine
  • Place of Presentation: 名古屋大学東山キャンパスESホール（愛知県）
  • Invited
• [Presentation] Genetically Encoded Bioluminescent Indicators for Real-Time Dual Imaging of Protease Activities in Living Cells (2011)
  • Author(s): A. Kanno, K. Nakazono, Y. Umezawa, T. Ozawa
  • Organizer: IUPAC International Congress on Analytical Sciences2011 (ICAS2011)
  • Place of Presentation: Kyoto
• [Presentation] Genetically Encoded Bioluminescent Indicators for Real-Time Dual Imaging of Protease Activities in Living Cells (2011)
  • Author(s): A. Kanno, K. Nakazono, Y . Umezawa, T. Ozawa
  • Organizer: PITTCON 2011, Atlanta
  • Place of Presentation: GA, U. S. A.
• [Presentation] Genetically Encoded Bioluminescent Indicators for Real-Time Dual Imaging of Protease Activities in Living Cells (2011)
  • Author(s): A. Kanno
  • Organizer: IUPAC International Congress on Analytical Sciences 2011 (ICAS2011)
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Bioluminescent Probes to Visualize Biological Functions in Living Cells (2009)
  • Author(s): A. Kanno, N.Hida, T. Ozawa
  • Organizer: 分子研研究会
  • Place of Presentation: Okazaki
• [Presentation] Cyclic Luciferase for Real-Time Sensing of Protease Activities in Living Mammals (2009)
  • Author(s): A. Kanno, Y. Umezawa, T. Ozawa
  • Organizer: Tokyo Conference 2009
  • Place of Presentation: Chiba
• [Presentation] 生体内のカスパーゼ-3活性を検出する環状ルシフェラーゼの開発 (2009)
  • Author(s): 菅野憲,梅澤喜夫,小澤岳昌
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 札幌
• [Book] 改訂六版 分析化学便覧，日本分析化学会編 (2012)
  • Author(s): 菅野憲
  • Total Pages: 880
  • Publisher: 丸善
• [Book] 「改訂六版分析化学便覧」,5.4.5細胞(日本分析化学会編) (2012)
  • Author(s): 菅野憲, 小澤岳昌
  • Total Pages: 880
  • Publisher: 丸善
• [Book] 改訂六版分析化学便覧 (2011)
  • Author(s): 菅野憲,小澤岳昌
  • Publisher: 日本分析化学会編
• [Remarks]
  • URL: http://www.chem.s.u-tokyo.ac.jp/users/analyt/index.html
• [Patent(Industrial Property Rights)] ACTIVATED PROTEASE INDICATOR (2010)
  • Inventor(s): UMEZAWA, Yoshio; OZAWA, Takeaki
  • Industrial Property Rights Holder: 東京大学
  • Acquisition Date: 2010-02-17