Correlation analysis by electrochemical detection of gene methylation detection and telomerase activity of tumor cells

• Project/Area Number: 22750072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: SHINOBU Sato 九州工業大学, 大学院・工学研究院, 助教 (80510677)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: テロメラーゼ / TERT / DNAメチレーション / 電気化学 / メチレーション

Research Abstract

Telomerase is an enzyme which can elongate telomere DNAs and is a good candidate for a cancer marker because of its specific expression in cancer cells. The promoter region of the hTERT gene, encoding for the catalytic subunit of human telomerase, has been located in a CpG island and may therefore be regulated at least in part by DNA methylation. We analyzed the methylation status of five CpG sites within the hTERT promoter core region by FND-based electrochemical hybridization assay and electrochemical telomerase activity assay in lysates of oral cancer patients.

Research Products

• [Journal Article] Electrochemical Diagnosis for Tongue Cancer : Telomerase Assay Based on Ferrocenylnaphthalene Diimide and Disposable Electrode Chips (2012)
  • Author(s): 佐藤しのぶ(他7名、1番目)
  • Journal Title: BUNSEKI KAGAKU Volume: 61 Issue: 3 Pages: 243-250
  • DOI: 10.2116/bunsekikagaku.61.243
  • NAID: 10030135417
  • ISSN: 0525-1931
  • Peer Reviewed
• [Journal Article] Detection of an aberrant methylation of CDH4 gene in PCR product by ferrocenylnaphthalene diimide-based electrochemical hybridization assay (2012)
  • Author(s): Shinobu Sato(他3名、1番目)
  • Journal Title: Analytica Chimica Acta Volume: 715巻 Pages: 42-48
  • DOI: 10.1016/j.aca.2011.12.010
  • Peer Reviewed
• [Journal Article] Shigeori Takenaka PCR-free telomerase assay using Chronocoulometry coupled with RuHex (2012)
  • Author(s): Shinobu Sato
  • Journal Title: Analytical Chemistry Volume: 84巻 Issue: 3 Pages: 1772-1775
  • DOI: 10.1021/ac202233m
  • Peer Reviewed
• [Journal Article] Electrochemical detection of aberrant methylated gene using naphthalene diimide derivative carrying four ferrocene moieties (2010)
  • Author(s): Shinobu Sato(他2名、1番目)
  • Journal Title: Journal of Organometallic Chemistry Volume: 695巻 Issue: 15-16 Pages: 1858-1862
  • DOI: 10.1016/j.jorganchem.2010.04.034
  • Peer Reviewed
• [Journal Article] Electrochemical detection of aberrant methylated gene using naphthalene diimide derivative carrying four ferrocene moieties (2010)
  • Author(s): Shinobu Sato
  • Journal Title: Journal of Organometallic Chemistry Volume: 695 Pages: 1858-1862
  • Peer Reviewed
• [Presentation] PCRを必要としない電気化学的テロメラーゼ検出法による口腔癌診断の試み (2012)
  • Author(s): 佐藤しのぶ(他6名、1番目)
  • Organizer: 日本化学会第92春季大会
  • Place of Presentation: 慶応義塾大学(東京都)
  • Year and Date: 2012-03-26
• [Presentation] Chronocoulometric telomerase assay (2012)
  • Author(s): Shinobu Sato, Shigeori Takenaka
  • Organizer: 5th International Symposium of Nanomedicine
  • Place of Presentation: 名古屋大学ESホール(愛知県)
  • Year and Date: 2012-03-16
• [Presentation] Chronocoulometric telomerase assay (2012)
  • Author(s): S.Sato, S.Takenaka
  • Organizer: 5th International Symposium of Nanomedicine
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] PCRを必要としない電気化学的テロメラーゼ検出法による口腔癌診断の試み (2012)
  • Author(s): 佐藤しのぶ, 森久美子, 児玉正明, 土生学, 西原達次, 冨永和宏, 竹中繁織
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学(神奈川県)
• [Presentation] テロメラーゼ活性を指標とした簡易口腔癌診断装置の開発 (2012)
  • Author(s): 佐藤しのぶ, 森久美子, 児玉正明, 土生学, 西原達次, 冨永和宏, 竹中繁織
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学(神奈川県)
• [Presentation] PCR-free electrochemical telomerase assay (2011)
  • Author(s): Shinobu Sato(他3名、2番目)
  • Organizer: Third International Meeting on G-Quadruplex and G-assembly
  • Place of Presentation: the Grand Hotel Vesuvio(イタリア)
  • Year and Date: 2011-06-29
• [Presentation] Cancer diagnosis based on PCR-free electrochemical telomerase assay (2011)
  • Author(s): Shinobu Sato(他7名、1番目)
  • Organizer: ICAS 2011(IUPAC 2011国際分析科学会議)
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-05-26
• [Presentation] Tongue cancer diagnosis using FND-based electrochemical telomerase assay (2011)
  • Author(s): Shinobu Sato
  • Organizer: 日本化学会第91春年会・アジア国際シンポジウム
  • Place of Presentation: 神奈川大学(神奈川)
  • Year and Date: 2011-03-28
• [Presentation] Cancer diagnosis based on PCR-free electrochemical telomerase assay (2011)
  • Author(s): S.Sato, Y.Fukunaga, K.Mori, H.Endo, K.Morimoto, K.Tominaga, T.Nishihara, S.Takenaka
  • Organizer: ICAS 2011(IUPAC 2011 国際分析科学会議)
  • Place of Presentation: 国立京都国際会館(京都府)
• [Presentation] hTERT遺伝子のDNAメチル化電気化学的検出 (2011)
  • Author(s): 田中智基, 兼崎祐介, 佐藤しのぶ, 竹中繁織
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] フェロセンを利用した電気化学的バイオセンシングシステムの構築とその応用 (2010)
  • Author(s): 佐藤しのぶ
  • Organizer: FIBERフォーラム2010
  • Place of Presentation: 甲南大学(兵庫県)
  • Year and Date: 2010-12-25
• [Presentation] フェロセンを利用した電気化学的バイオセンシングシステムの構築とその応用 (2010)
  • Author(s): 佐藤しのぶ
  • Organizer: FIBERフォーラム2010
  • Place of Presentation: 兵庫県(甲南大学)
  • Year and Date: 2010-12-25
• [Presentation] Electrochemical detection of aberrant methylated genes by using ferrocenylnaphthalene diimide (2010)
  • Author(s): Shinobu Sato(他3名、1番目)
  • Organizer: The International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: ハワイコンベンションセンター(ハワイ)
  • Year and Date: 2010-12-18
• [Presentation] Electrochemical detection of telomerase activity using ferrocenylnaphthalene diimide (2010)
  • Author(s): Shinobu Sato(他6名、1番目)
  • Organizer: The International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: ワイコンベンションセンター(ハワイ)
  • Year and Date: 2010-12-17
• [Presentation] Electrochemical detection of aberrant methylated genes by using ferrocenylnaphthalene diimide (2010)
  • Author(s): Shinobu Sato
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Hawaii (Hawaii Convention Center)
  • Year and Date: 2010-12-17
• [Presentation] Electrochemical detection of nuclease activity with ferrocenyloligonucleotide-immobilized electrodes (2010)
  • Author(s): Shinobu Sato(他3名、1番目)
  • Organizer: The International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: ワイコンベンションセンター(ハワイ)
  • Year and Date: 2010-12-16
• [Presentation] Electrochemical detection of telomerase activity using ferrocenylnaphthalene diimide (2010)
  • Author(s): Shinobu Sato
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Hawaii (Hawaii Convention Center)
  • Year and Date: 2010-12-16
• [Presentation] FND-based electrochemical telomerase assay aiming at tongue cancer diagnosis (2010)
  • Author(s): Shinobu Sato
  • Organizer: 第三回バイオセンシング技術に関する日韓シンポジウム(3rd JKBT)
  • Place of Presentation: 福岡県(九州工業大学 戸畑キャンパス)
  • Year and Date: 2010-12-03
• [Presentation] FND-based electrochemical telomerase assay aiming at tongue cancer diagnosis (2010)
  • Author(s): Shinobu Sato
  • Organizer: 第三回バイオセンシング技術に関する日韓シンポジウム(3rd JKBT)
  • Place of Presentation: 九州工業大学(福岡県)
  • Year and Date: 2010-11-18
• [Presentation] PCR-Freeなテロメラーゼ活性検出の確立とその応用 (2010)
  • Author(s): 佐藤しのぶ(他5名、1番目)
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2010-09-26
• [Presentation] PCR-Freeなテロメラーゼ活性検出の確立とその応用 (2010)
  • Author(s): 佐藤しのぶ
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪府(大阪大学 豊中キャンパス)
  • Year and Date: 2010-09-25
• [Presentation] 電気化学的テロメラーゼ検出法による舌癌診断 (2010)
  • Author(s): 佐藤しのぶ(他6名、4番目)
  • Organizer: 第58回分析化学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2010-09-15
• [Presentation] 四本鎖DNA検出試薬としての種々のリンカー長を有するフェロセン化ナフタレンジイミド誘導体の性能評価 (2010)
  • Author(s): 佐藤しのぶ
  • Organizer: 分析化学討論会
  • Place of Presentation: 島根県(島根大学松江キャンパス)
  • Year and Date: 2010-05-16
• [Presentation] 四本鎖DNA検出試薬としての種々のリンカー長を有するフェロセン化ナフタレンジイミド誘導体の性能評価 (2010)
  • Author(s): 佐藤しのぶ(他2名、1番目)
  • Place of Presentation: 島根大学(島根県)
  • Year and Date: 2010-05-15
• [Presentation] フェロセン化ナフタレンジイミドを利用した電気化学的異常メチル化遺伝子の検出 (2010)
  • Author(s): 佐藤しのぶ(他3名、1番目)
  • Organizer: ナノ学会第8回大会
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2010-05-13
• [Presentation] Tongue cancer diagnosis using FND-based electrochemical telomerase assay (2010)
  • Author(s): Shinobu Sato
  • Organizer: 日本化学会第91春年会・アジア国際シンポジウム
  • Place of Presentation: 神奈川県(神奈川大学 横浜キャンパス)
  • Year and Date: 2010-03-28
• [Book] Electrochemical approaches to the study of DNA-drug interactions, Methods for Studying Nucleic Acid Drug Interactions (2011)
  • Author(s): Shinobu Sato、Shigeori Takenaka, M. Wanunu, Y. Tor Eds. CRC Press
• [Remarks]
  • URL: http://takenaka.che.kyutech.ac.jp
• [Remarks]
  • URL: http://takenaka.che.kyutech.ac.jp/2011/conf2011.html