The mechanism for length regulation of thick filament by muscle elastic protein connectin

• Project/Area Number: 22770065
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Chiba University
• Principal Investigator: HANASHIMA Akira 千葉大学, 大学院・理学研究科, 特任研究員 (70572981)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: コネクチン / 横紋筋 / 弾性タンパク質 / 巨大タンパク質 / サルコメア / タイチン / ミオシン / 分子定規 / 太いフィラメント

Research Abstract

We found a novel protein that binds to muscle elastic protein connectin. Our analysis indicates this protein locates at the edge of myosin filament and neuron-muscular junction on striated muscles, and the gene of this protein includes twenty-five exons and produces five splicing isoforms. Additionally, we identified the binding proteins and a break-down protein of novel protein, and also found the existence of this protein in various animals including human and Drosophila.

Research Products

• [Journal Article] Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways (2013)
  • Author(s): Rudolf R, Bogomolovas J, Strack S, Choi K, Khan MM, Wagner A, Brohm K, Hanashima A, Gasch A, Labeit D, Labeit S
  • Journal Title: AGE Volume: (in press) Issue: 5 Pages: 1663-1674
  • DOI: 10.1007/s11357-012-9468-9
  • Peer Reviewed
• [Journal Article] Actin capping proteins, CapZ (beta-actinin) and tropomodulin in amphioxus striated muscle (2012)
  • Author(s): Bao Y, Kake T, Hanashima A, Nomiya Y, Kubokawa K, Kimura S
  • Journal Title: GENE Volume: 510 Pages: 78-86
  • Peer Reviewed
• [Journal Article] Genomic- and protein-based approaches for connectin (titin) identification in the ascidian Ciona intestinalis (2012)
  • Author(s): Hanashima, A., Ogasawara, M., Nomiya, Y., Sasaki, T., Bao, Y., Kimura, S.
  • Journal Title: Methods Volume: Vol. 56(1) Issue: 1 Pages: 18-24
  • DOI: 10.1016/j.ymeth.2011.12.010
  • Peer Reviewed
• [Journal Article] Actin capping proteins, CapZ (β-actinin) and tropomodulin in amphioxus striated muscle (2012)
  • Author(s): Bao, Y., Kake, T., Hanashima, A., Nomiya, U., Kubokawa, K. and Kimura, S.
  • Journal Title: Gene Volume: Vol. 510 (1) Issue: 1 Pages: 78-86
  • DOI: 10.1016/j.gene.2012.07.081
  • Peer Reviewed
• [Journal Article] Genomic- and protein-based approaches for connectin (titin)identification in the ascidian Ciona intestinalis (2012)
  • Author(s): Hanashima, et al.
  • Journal Title: Methods Volume: 56 Pages: 18-24
  • Peer Reviewed
• [Journal Article] Amphioxus connectin exhibits merged structure as invertebrate connectin in I-band region and vertebrate connectin in A-band region (2011)
  • Author(s): Ohtsuka, S., Hanashima, A., Kubokawa, K., Bao, Y., Tando, Y., Kohmaru, J., Nakaya, H., Maruyama, K. and Kimura, S.
  • Journal Title: J. Mol. Biol Volume: Vol. 409 (3) Issue: 3 Pages: 415-426
  • DOI: 10.1016/j.jmb.2011.04.010
  • Peer Reviewed
• [Journal Article] Amphioxus connectin exhibits merged structure as invertebrate connectin in I-band region and vertebrate connectin in A-band region (2011)
  • Author(s): Ohtsuka, et al.
  • Journal Title: J Mol Biol Volume: 409 Pages: 415-426
  • Peer Reviewed
• [Journal Article] Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin (2010)
  • Author(s): Chitose, R., Watanabe, A., Asano, M., Hanashima, A., Sasano, K., Bao, Y.,Maruyama, K. and Kimura, S.
  • Journal Title: J. Biomed. Biotechnol Volume: 2010 Pages: 108495-108495
  • DOI: 10.1155/2010/108495
  • Peer Reviewed
• [Journal Article] Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin (2010)
  • Author(s): Chitose, et al.
  • Journal Title: J Biomed Biotechnol Volume: 2010 Pages: 108495-108495
  • Peer Reviewed
• [Presentation] プラナリアのコネクチン様タンパク質の配列 (2012)
  • Author(s): 佐々野耕平、○倉沢正義、花島章、包玉龍、中内祐二、木村澄子
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] 軟体動物横紋筋のコネクチン様タンパク質 (2012)
  • Author(s): ○包玉龍、花島章、袖山文彰、木村澄子
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-22
• [Presentation] 脊椎動物のゲノム中におけるコネクチン遺伝子数の変遷 (2012)
  • Author(s): ○花島章、木村澄子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] ホヤ心筋ネブリンの一次構造解析 (2012)
  • Author(s): ○西川彰、浜岡知哉、小笠原道生、花島章、木村澄子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] 刺胞動物のコネクチン様タンパク質 (2012)
  • Author(s): ○春山綾菜、花島章、木村澄子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] ウニのコネクチン様タンパク質 (2012)
  • Author(s): ○佐々木智子、松浦哲、花島章、野宮唯、木村澄子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] Genomic- and Protein-Based Approaches for Connectin-Like Protein Identification in Amphioxus and Ascidian (2012)
  • Author(s): ○ Hanashima A, Bao Y, Kimura S
  • Organizer: BIT's 3rd World DNA and Genome day
  • Place of Presentation: Xi'an, China
  • Year and Date: 2012-04-28
• [Presentation] 無脊椎動物平滑筋のコネクチン様タンパク質 (2012)
  • Author(s): ○包玉龍、花島章、松浦哲、木村澄子
  • Organizer: 第49回生体運動研究合同班会議
  • Place of Presentation: 筑波
  • Year and Date: 2012-01-06
• [Presentation] カイメン、ヒドラのコネクチン様タンパク質の探索 (2011)
  • Author(s): 日高暢也、○木村澄子、花島章
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] ホヤのコネクチン様タンパク質 (2011)
  • Author(s): ○佐々木智子、野宮唯、花島章、包玉龍、小笠原道生、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] 扁形動物ナミウズムシDugesiajaponicaのコネクチン様タンパク質 (2011)
  • Author(s): ○佐々野耕平、花島章、包玉龍、中内祐二、木村澄子
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] コネクチン様タンパク質の多様性 (2011)
  • Author(s): ○佐々野耕平、花島章、大塚聡、松浦哲、幸丸純貴、木村澄子
  • Organizer: 第48回生体運動研究合同班会議
  • Place of Presentation: 大阪
  • Year and Date: 2011-01-07
• [Presentation] Amphioxus striated muscle has beta-actinin and tropomodulin (2010)
  • Author(s): ○ Bao Y, Kake T, Hanashima A,Kubokawa K, Tando Y, Kimura S
  • Organizer: International Conference on Bioinformatics
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-26
• [Presentation] The connectin-like protein gene of amphioxus striated muscle (2010)
  • Author(s): Ohtsuka S, Hanashima A, KubokawaK, Tando Y, ○ Kimura S.
  • Organizer: International Conference on Bioinformatics
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-09-26
• [Presentation] ホタテ貝横紋筋のコネクチン様タンパク質 (2010)
  • Author(s): ○包玉龍、黒澤元、花島章、木村澄子
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] ウニコネクチン様タンパク質のドメイン構造 (2010)
  • Author(s): ○松浦哲、花島章、包玉龍、木村澄子
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] プラナリアのコネクチン様タンパク質の探索 (2010)
  • Author(s): ○佐々野耕平、花島章、中内祐二、木村澄子
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] 頭索動物ナメクジウオのトロポモジュリンの同定 (2010)
  • Author(s): ○包玉龍、掛武威、花島章、木村澄子
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-21
• [Presentation] Genomic- and Protein-Based Approaches for Connectin-Like Protein Identification in Amphioxus and Ascidian.
  • Author(s): Hanashima A
  • Organizer: BIT’s 3rd World DNA and Genome day.
  • Place of Presentation: Xi’an, China
  • Invited
• [Presentation] 脊椎動物のゲノム中におけるコネクチン遺伝子数の変遷
  • Author(s): 花島章、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
• [Presentation] ウニのコネクチン様タンパク質
  • Author(s): 佐々木智子、松浦哲、花島章、野宮唯、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
• [Presentation] 刺胞動物のコネクチン様タンパク質
  • Author(s): 春山綾菜、花島章、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
• [Presentation] ホヤ心筋ネブリンの一次構造解析
  • Author(s): 西川彰、浜岡知哉、小笠原道生、花島章、木村澄子
  • Organizer: 日本動物学会 第83回大会
  • Place of Presentation: 大阪
• [Presentation] 軟体動物横紋筋のコネクチン様タンパク質
  • Author(s): 包玉龍、花島章、袖山文彰、木村澄子
  • Organizer: 日本生物物理学会 第50回年会
  • Place of Presentation: 名古屋
• [Presentation] プラナリアのコネクチン様タンパク質の配列
  • Author(s): 佐々野耕平、倉沢正義、花島章、包玉龍、中内祐二、木村 澄子
  • Organizer: 日本生物物理学会 第50回年会
  • Place of Presentation: 名古屋