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Effect of molecular chaperone on misfolding protein aggregation

Research Project

Project/Area Number 22770117
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

ZAKO Tamotsu  独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (50399440)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsアミロイド / 分子シャペロンタンパク質 / ミスフォールディングタンパク質凝集 / プレフォルディン / 可溶性オリゴマー / 細胞毒性 / タンパク質ミスフォールディング病 / アルツハイマー病 / アミロイドβ / タンパク質凝集
Research Abstract

Protein misfolding disease, such as Alzheimer's' disease(AD), is caused by misfolded protein aggregates that induce molecular stress and interfere with cellular function. We examined effect of molecular chaperone prefoldin(PFD) on aggregation of amyloid beta and poly glutamine protein that cause AD and Huntington disease, respectively. We found that PFD could inhibit their aggregation in vitro.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2012 2011 2010

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (24 results)

  • [Journal Article] Cell interaction study of amyloid using luminescent conjugated polythiophene : Implication that amyloid cytotoxicity is correlated with prolonged cellular binding2012

    • Author(s)
      Tamotsu Zako
    • Journal Title

      ChemBioChem

      Volume: (in press) Issue: 3 Pages: 358-363

    • DOI

      10.1002/cbic.201100467

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Naked-eye Detection of Amyloid Aggregates Using Gold Nanoparticles Modified with Amyloid Beta Antibody2012

    • Author(s)
      Masafumi Sakono
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 1 Pages: 73

    • DOI

      10.2116/analsci.28.73

    • NAID

      130004827182

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immobilized Insulin Amyloid Enhances Cell Adhesion and Proliferation due to Interaction with Fibronectin2011

    • Author(s)
      Sakono M, Akiyama S, Zako T., Sakaki S, Waku T, Tanaka N, Maeda M
    • Journal Title

      Chem. Lett

      Volume: 40 Pages: 315-317

    • NAID

      10027815991

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of two morphologically different fibrillar and filamentous insulin amyloids as a biomaterial for cell culture surfaces2011

    • Author(s)
      Sakono M, Akiyama S, Zako T., Sakaki S, Waku T, Tanaka N, Maeda M
    • Journal Title

      React. Funct. Polymer

      Volume: 71 Pages: 324-328

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immobilized Insulin Amyloid Enhances Cell Adhesion and Proliferation due to Interaction with Fibronectin2011

    • Author(s)
      Masafumi Sakono
    • Journal Title

      Chemistry Letters

      Volume: 40 Pages: 315-317

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of two morphologically different fibrillar and filamentous insulin amyloids as a biomaterial for cell culture surfaces2011

    • Author(s)
      Masafumi Sakono
    • Journal Title

      Reactive Functional Polymers

      Volume: 71 Issue: 3 Pages: 324-328

    • DOI

      10.1016/j.reactfunctpolym.2010.10.012

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Size-selective recognition of gold nanoparticles by a molecular chaperone2010

    • Author(s)
      Sakono M, Zako T., Drakulic S, Valpuesta J. M, Yohda M and Maeda M
    • Journal Title

      Chem. Phys. Lett

      Volume: 501 Pages: 108-112

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements2010

    • Author(s)
      Sahlan M., Kanzaki T., Zako T., Maeda M. and Yohda M.
    • Journal Title

      Biochimica et Biophysica Acta-Proteins and Proteomics

      Volume: 1804 Pages: 1810-1816

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin2010

    • Author(s)
      Sahlan M., Zako T., Tai Phan The, Ohtaki A., Noguchi K., Maeda M., Miyatake H., Dohmane N. and Yohda M.
    • Journal Title

      J. Mol. Biol

      Volume: 399 Pages: 628-636

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bio-Supramolecular Photochirogenesis with Molecular Chaperone : Enantiodifferentiating Photocyclodimerization of 2-Anthracenecarboxylate Mediated by Prefoldin2010

    • Author(s)
      Bando K., Zako T., Sakono M., Maeda M., Wada T., Nishijima M., Fukuhara G., Yang C., Mori T., Pace TCS., Bohne C. and Inoue Y.
    • Journal Title

      Photochem. & Photobiol. Sci

      Volume: 9 Pages: 655-60

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amyloid oligomers : Formation and toxicity of Abeta oligomers2010

    • Author(s)
      Sakono M. and Zako T.
    • Journal Title

      FEBS J.

      Volume: 277 Pages: 1348-1358

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyperthermophilic archaeal prefoldin shows refolding activity at low temperature2010

    • Author(s)
      Zako T., Banba S., Sahlan M., Sakono M., Terada N., Yohda M. and Maeda M.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 391 Pages: 467-470

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyperthermophilic archaeal prefoldin shows refolding activity at low temperature2010

    • Author(s)
      Tamotsu Zako
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 391 Pages: 467-470

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation and toxicity of Abeta oligomers2010

    • Author(s)
      Masafumi Sakono
    • Journal Title

      FEBS Journal

      Volume: 277 Pages: 1348-1358

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amyloid oligomers2010

    • Author(s)
      Tamotsu Zako
    • Journal Title

      FEBS Journal

      Volume: 277 Pages: 1347-1347

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bio-Supramolecular Photochirogenesis with Molecular Chaperone2010

    • Author(s)
      Kazuki Bando
    • Journal Title

      Photochemical & Photobiological Sciences

      Volume: 9 Pages: 655-660

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin2010

    • Author(s)
      Muhamad Sahlan
    • Journal Title

      Journal of Molecular Biology

      Volume: 399 Pages: 628-636

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements2010

    • Author(s)
      Muhamad Sahlan
    • Journal Title

      Biochimica et Biophysica Acta.- Proteins and Proteomics

      Volume: 1804 Pages: 1810-1816

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Size-selective recognition of gold nanoparticles by a molecular chaperone2010

    • Author(s)
      Masafumi Sakono
    • Journal Title

      Chemical Physics Letters

      Volume: 501 Pages: 108-112

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 分子シャペロンによるアミロイドベークオリゴマーの検出2012

    • Author(s)
      Masafumi Sakono
    • Organizer
      化学工学会第77年会
    • Place of Presentation
      工学院大学(東京)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Function and application of protein molecules and bio-nanoparticles2011

    • Author(s)
      Tamotsu Zako and Mizuo Maeda
    • Organizer
      Japan-Canada Nanotechnology Workshop
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2011-11-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 近赤外光を用いたバイオイメージングのためのナノ粒子開発とその応用2011

    • Author(s)
      座古保
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of new photon counting histogram method towards precise analysis of oligomerization2011

    • Author(s)
      Naofumi Terada, Tamotsu Zako, Masafumi Sakono and Mizuo Maeda
    • Organizer
      第49回生物物理年会
    • Place of Presentation
      姫路
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of new photon counting histogram method towards precise analysis of oligomerization2011

    • Author(s)
      Naofumi Terada
    • Organizer
      第49回生物物理年会
    • Place of Presentation
      兵庫県立大学(姫路)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Noodle'-like filaments of bovine insulin andβ2 microglobulin were non-cytotoxic due to abbreviated cell binding2011

    • Author(s)
      Tamotsu Zako, Masafumi Sakono, Takahiro Kobayashi, K. Peter R. Nilsson, Per Hammarstrom, Mikael Lindgren and Mizuo Maeda
    • Organizer
      EMBO meeting 2011
    • Place of Presentation
      Vienna
    • Year and Date
      2011-09-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Noodle'-like filaments of bovine insulin and ss2 microglobulin were non-cytotoxic due to abbreviated cell binding2011

    • Author(s)
      Tamotsu Zako
    • Organizer
      EMBO meeting
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2011-09-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] The molecular chaperone prefoldin can capture aggregation prone states of A-beta and might play a role in the progress of Alzheimers disease2010

    • Author(s)
      Karin Sorgjerd, Tamotsu Zako, Masafumi Sakono, Peter Stirling, Michel Leroux, Mizuo Maeda
    • Organizer
      Pacifichem2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-19
    • Related Report
      2011 Final Research Report
  • [Presentation] The molecular chaperone prefoldin can capture aggregation prone states of A-beta and might play a role in the progress of Alzheimers disease2010

    • Author(s)
      Sorgjed Karin
    • Organizer
      PacifiChem2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Toxic amyloid oligomer detection system using molecular chaperone2010

    • Author(s)
      Masafumi Sakono, Tamotsu Zako, Masafumi Yohda and Mizuo Maeda
    • Organizer
      Pacifichem2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Toxic amyloid oligomer detection system using molecular chaperone2010

    • Author(s)
      Masafumi Sakono
    • Organizer
      PacifiChem2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Supramolecular photochirogenesis with biomolecular hosts2010

    • Author(s)
      Y. Inoue, M. Nishijima, C. Yang, T. Mori, T. Wada, K. Bando, T. Zako, M. Sakon, M. Maeda, T. Pace, C. Bohne
    • Organizer
      Pacifichem2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Supramolecular photochirogenesis with biomolecular hosts2010

    • Author(s)
      Yoshihisa Inoue
    • Organizer
      PacifiChem2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子シャペロンPrefoldinは変異ハンチンチンのオリゴマーおよび凝集体形成を阻害して細胞死を減少させる2010

    • Author(s)
      田代絵梨佳,武藤秀樹,座古保,宮澤誠,北浦廣剛,北村朗,久保田広志,前田瑞夫,金城政孝,有賀寛芳
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-06
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Molecular chaperone prefoldin blocks the formation of mutant Huntingtin oligomer and inclusion body, resulting in reduction of its cytotoxity2010

    • Author(s)
      Erika Tashiro, Hideki Muto, Tamotsu Zako, Makoto Miyazawa, Hirotake Kitaura, Akira Kitamura, Hiroshi Kubota, Mizuo Maeda, Masataka Kinjo, Hiroyoshi Ariga
    • Organizer
      Neuroscience
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2010-11-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Amyloid Beta Oligomer Studied By photon counting histogram2010

    • Author(s)
      Naofumi Terada, Tamotsu Zako, Masafumi Sakono and Mizuo Maeda
    • Organizer
      第48回日本生物物理学会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-20
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Formation of non-toxic Abeta amyloids by small heat shock protein(sHsp) under stress condition2010

    • Author(s)
      Tamotsu Zako, Masafumi Sakono, Arata Utsum, Chika Sugino, Masafumi Yohda, Mizuo Maeda
    • Organizer
      EMBO meeting
    • Place of Presentation
      Barcelona Spain
    • Year and Date
      2010-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Formation of non-toxic Abeta amyloids by small heat shock protein (sHsp) under stress condition2010

    • Author(s)
      Tamotsu Zako
    • Organizer
      EMBO meeting
    • Place of Presentation
      Barcelona Spain
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子シャペロンをめぐる分子間相互作用の解析2010

    • Author(s)
      養王田正文、阿部哲也、Sahlan Muhamad, Phan The tai,神埼太郎、岡俊彦、座古保、前田瑞夫
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-06-17
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] ポリグルタミンタンパク質凝集における分子シャペロンプレフォルディンの役割2010

    • Author(s)
      伊藤禎宣、座古保、Karin Sorgjerd、寺田尚史、田代絵梨佳、北村朗、久保田広志、武藤秀樹、金城政孝、有賀寛芳、前田瑞夫
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-06-16
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] The Molecular Chaperone Prefoldin can Capture Aggregation Prone States of A-Beta and Might Play a Role in the Progress of Alzheimer's Disease2010

    • Author(s)
      Karin Sorgjerd, Tamotsu Zako, Masafumi Sakono, Peter Stirling, Michel Leroux, Mizuo Maeda
    • Organizer
      第59回高分子学会年次大会
    • Place of Presentation
      仙台
    • Year and Date
      2010-05-24
    • Related Report
      2011 Final Research Report
  • [Presentation] The molecular chaperone prefoldin can capture aggregation prone states of A-beta and might play a role in the progress of Alzheimers disease2010

    • Author(s)
      Sorgjed Karin
    • Organizer
      第59回高分子学会年次大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-05-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Formation of non-toxic Abeta fibrils by small heat shock protein(sHsp) under stress condition2010

    • Author(s)
      Tamotsu Zako, Masafumi Sakono, Arata Utsum, Chika Sugino, Masafumi Yohda, Mizuo Maeda
    • Organizer
      Cold Spring Harbor Meeting Molecular Chaperons & Stress responses
    • Place of Presentation
      New York, USA
    • Year and Date
      2010-05-07
    • Related Report
      2011 Final Research Report
  • [Presentation] Formation of non-toxic Abeta fibrils by small heat shock protein (sHsp) under stress condition2010

    • Author(s)
      Tamotsu Zako
    • Organizer
      Cold Spring Harbor Meeting Molecular Chaperons & Stress responses
    • Place of Presentation
      New York, USA
    • Year and Date
      2010-05-07
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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