| Project/Area Number |
22770153
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | University of Hyogo |
|---|
Principal Investigator |
YAMAMOTO Hitoshi 兵庫県立大学, 大学院・生命理学研究科, 研究員 (40419007)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 1分子計測 / 操作 / 膜タンパク質 / 膜透過 / 一分子計測 / 蛋白質 / 生体膜 / 膜透過ダイナミクス / 生物物理 |
|---|
| Research Abstract |
Protein translocation and integration are essential processes by which proteins are transported to their proper destinations. These processes proceed on the endoplasmic reticulum membrane in eukaryotic cells. In this study, we aimed at single molecule analysis of protein translocation dynamics. Protein translocation intermediate were synthesized with using cell free protein synthesis system and microsome, and labeled with fluorescent dye by suppressor-tRNA methods. The fluorescence and quenching were successfully observed by using total internal reflection fluorescence microscopy. However, due to the low S/N ratio caused by high background light, low fluorescence intensity and short life time, it was found that effective fluorescence intensity change was hardly observed. Thus, improving the S/N ratio remained to be as future work.
|
