Mutagenesis analysis of hydrogen bond network formed in bovine heart cytochrome c oxidase
| Project/Area Number |
22770155
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | University of Hyogo |
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Principal Investigator |
AMINAKA Ryohta 兵庫県立大学, 大学院・生命理学研究科, 助教 (30467724)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | タンパク質 / 核酸の構造 / 動態 / 機能 / 酵素 / 核酸 / チトクロムc酸化酵素 |
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| Research Abstract |
We carried out mutagenesis analyses of a hydrogen bond network, designated as D-pathway, formed in the bovine heart cytochrome c oxidase employing HeLa cell bovine/human hybrid enzyme expression system developed by us. A pathway corresponding to this D-pathway is also found in the bacterial enzymes, and has been proposed to convey both pumping and water forming protons. The structures of these two pathways are almost identical to each other and amino acid residues essential to the bacterial enzymes are conserved in the bovine enzyme. Hence, it has been thought that these two have the same function, thereby opposing to the proton-pumping pathway of the bovine enzyme proposed by us. The results of this study strongly suggest D-pathway is not for pumping proton but for water forming proton.
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Report
(3 results)
Research Products
(14 results)
