| Project/Area Number |
22770177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | セントロメア / クロマチン / CENP-A / RSF / 細胞周期 / DT40細胞 / 染色体分配 / 免疫沈降 |
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| Research Abstract |
Previously, I found that the RSF complex transiently interacts with CENP-A and acts in CENP-A incorporation into centromeres. Therefore, I wanted to determine 1) how the RSF complex targets the centromere, 2) which are the synergic factors that help the RSF complex in the stabile retention of CENP-A chromatin and 3) how the RSF complex is involved in kinetochore assembly. To address these questions, I performed anti-FLAG chromatin immunoprecipitation of DT40 cells stably expressing FLAG-Rsf-I and found that RSF-associated DNA distributes along the genome, but a specific sharp peak overlaps the peak of the CENP-A-associated DNA. In addition, I found that the RSF complex mainly interacts with the FACT complex, which is another chromatin remodeling complex that we also spotted at centromere and found that interacts with CENP-A. I demonstrated that the Rsf1 amount was reduced in cells with knockout of FACTp80(SSRP1).
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