Analysis of the hyaluronan-dependent signaling in vertebrateheart formation

• Project/Area Number: 22770204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: ITO Yuzuru 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 研究チーム長 (30500079)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 発生・分化 / マイクロアレイ / 糖鎖 / 再生医学 / シグナル伝達

Research Abstract

I identified many novel genes expected as factor belong to hyaluronan-dependent signaling pathway. 7 genes were detected in heart. 6 genes were detected in blood vessel. 4 genes were detected in liver. 12 genes were detected in blood cells. These three ti

Research Products

• [Journal Article] rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells. (2013)
  • Author(s): Onuma Y
  • Journal Title: Biochem. Biopys. Res. Commun. Volume: 431 Issue: 3 Pages: 524-529
  • DOI: 10.1016/j.bbrc.2013.01.025
  • Peer Reviewed
• [Journal Article] mNanog possesses dorsal mesoderm-inducing ability by modulating bothBMP and Activin/nodal signaling in Xenopus ectodermal cells (2012)
  • Author(s): Aya Miyazaki
  • Journal Title: PLoS One Volume: 7巻 Issue: 10 Pages: e46630-e46630
  • DOI: 10.1371/journal.pone.0046630
  • Peer Reviewed
• [Journal Article] KDEL tagging: a method for generating dominant-negative inhibitors of the secretion of TGF-beta superfamily proteins (2012)
  • Author(s): Shinya Matsukawa
  • Journal Title: The International Journal of Developmental Biology Volume: 56巻 Issue: 5 Pages: 351-356
  • DOI: 10.1387/ijdb.123514sm
  • Peer Reviewed
• [Journal Article] Structural and quantitative evidence for dynamic glycome shift upon production of human induced pluripotent stem cells. (2012)
  • Author(s): Hasehira K
  • Journal Title: Mol Cell Proteomics. Volume: 11 Issue: 12 Pages: 1913-1923
  • DOI: 10.1074/mcp.m112.020586
  • Peer Reviewed
• [Journal Article] Bioluminescent Capsules for Live-Cell Imaging. (2012)
  • Author(s): Kim SB
  • Journal Title: Bioconjugate Chem. Volume: 23 Issue: 11 Pages: 2221-2228
  • DOI: 10.1021/bc300323x
  • Peer Reviewed
• [Journal Article] Ubc9 negatively regulates BMP-mediated osteoblastic differentiation in cultured cells (2012)
  • Author(s): Yukita A, Hosoya A, Ito Y, Katagiri T, Asashima M, Nakamura H
  • Journal Title: Bone Volume: 50 Pages: 1092-1099
  • Peer Reviewed
• [Journal Article] 発生学から見た再生医療 (2011)
  • Author(s): 浅島誠、 伊藤弓弦、栗崎晃
  • Journal Title: 再生 医療 Volume: 10(3) Pages: 17-33
  • URL: http://med.m-review.co.jp/magazine/detail1/J27_10_3_17-33.html
• [Journal Article] Development of Ca(2)(+) signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation (2011)
  • Author(s): Takano K., Obata S., Komazaki S., Masumoto M., Oinuma T., Ito Y., Ariizumi T., Nakamura H., Asashima M.
  • Journal Title: Dev.Growth Differ. Volume: 53 Issue: 1 Pages: 37-47
  • DOI: 10.1111/j.1440-169x.2010.01220.x
  • NAID: 10027899242
  • Peer Reviewed
• [Journal Article] ycome diagnosis of human induced pluripotent stem cells using lectin microarra (2011)
  • Author(s): Tateno H., Toyoda M., Saito S., Onuma Y., Ito Y., Hiemori K., Fukumura M., Nakasu A., Nakanishi M., Ohnuma K., Akutsu H., Umezawa A., Horimoto K., Hirabayashi J., Asashima M.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 20345-20353
  • Peer Reviewed
• [Journal Article] Insulin biosynthesis in neuronal progenitors derived from adult hippocampus and the olfactory bulb (2011)
  • Author(s): Kuwabara T., Kagalwala M.N., Onuma Y., Ito Y., Warashina M., Terashima K., Sanosaka T., Nakashima K., Gage F.H., Asashima, M
  • Journal Title: EMBO Mol.Med. Volume: 3 Issue: 12 Pages: 742-754
  • DOI: 10.1002/emmm.201100177
  • Peer Reviewed
• [Journal Article] 発生学から見た再生医療 (2011)
  • Author(s): 浅島誠、伊藤弓弦、栗崎晃
  • Journal Title: 再生医療 Volume: 10 Pages: 17-33
• [Journal Article] Development of Ca(2)(+) signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation. (2011)
  • Author(s): Takano K, Obata S, Komazaki S, Masumoto M, Oinuma T, Ito Y, Ariizumi T, Nakamura H, Asashima M.
  • Journal Title: Dev.Growth Differ. Volume: 53 Pages: 37-47
  • NAID: 10027899242
  • Peer Reviewed
• [Journal Article] Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation (2010)
  • Author(s): Yamagishi M, Ito Y, Ariizumi T, Komazaki S, Danno H, Michiue T, Asashima M
  • Journal Title: Dev Growth Differ Volume: 52 Issue: 7 Pages: 665-75
  • DOI: 10.1111/j.1440-169x.2010.01204.x
  • NAID: 10027566245
  • Peer Reviewed
• [Journal Article] Intensely Fluorescent Azobenzenes : Synthesis, Crystal Structures, Effects of Substituents, and Application to Fluorescent Vital Stain. (2010)
  • Author(s): Yoshino, J., Furuta, A., Kambe, T., Itoi, H., Kano, N., Kawashima, T., Ito, Y., Asashima, M.
  • Journal Title: Chem.Eur.J. Volume: 16 Pages: 5026-5035
  • Peer Reviewed
• [Journal Article] Cloning of noggin gene from hydra and analysis of its functional conser vation using Xenopus laevis embryos. (2010)
  • Author(s): Chandramore, K., Ito, Y., Takahashi, S., Asashima, M., Ghaskadb, S.
  • Journal Title: Evol.Dev. Volume: 12 Pages: 267-274
  • Peer Reviewed
• [Journal Article] Claudin5 genes encoding tight junction proteins are required for Xenopus heart formation. (2010)
  • Author(s): Yamagishi, M., Ito, Y., Ariizumi, T., Komazaki, S., Danno, H., Michiue, T., Asashima, M.
  • Journal Title: Dev.Growth Differ. Volume: 52 Pages: 665-675
  • NAID: 10027566245
  • Peer Reviewed
• [Journal Article] 「DomeGene」宇宙環境が両生類培養細胞に及ぼす影響 (2010)
  • Author(s): 浅島誠、中島由郎、矢野幸子、内田智子、嶋津徹、伊藤弓弦
  • Journal Title: 生物工学 Volume: 88 Pages: 285-287
  • NAID: 110007658221
• [Presentation] iPS細胞等幹細胞評価基盤技術開発に向けたアプローチ (2013)
  • Author(s): 伊藤 弓弦
  • Organizer: 第3回インテレクチャルカフェ広島
  • Place of Presentation: 広島
  • Invited
• [Presentation] ヒトES/iPS細胞特異的な新規細胞表面染色マーカーの開発 (2013)
  • Author(s): 伊藤 弓弦
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 高感度糖鎖プロファイラー・レクチンマイクロアレイによる新規未分化マーカーの発見 (2013)
  • Author(s): 舘野 浩章
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] ヒトES・iPS細胞の無酵素培養 (2013)
  • Author(s): 大沼清
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 初期心臓形成に関与するヒアルロン酸経路の解析 (2012)
  • Author(s): 岩本 麻未
  • Organizer: 第11回産総研・産技連 LS-BT 合同研究発表会
  • Place of Presentation: 産総研(茨城)
  • Year and Date: 2012-01-31
• [Presentation] 幹細胞評価基盤技術開発に向けたアプローチ (2012)
  • Author(s): 伊藤弓弦
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: (独)産業技術総合研究所(茨城県)(招待講演)
  • Year and Date: 2012-01-31
• [Presentation] 持続発現型RNAベクターを用いたヒトiPS細胞樹立法の確立 (2012)
  • Author(s): 大高真奈美、佐野将之、西村健、高安聡子、小沼泰子、中島由郎、伊藤弓弦、池原譲、浅島誠、中西真人
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: (独)産業技術総合研究所(茨城県)
  • Year and Date: 2012-01-31
• [Presentation] 心臓形成におけるMEF2cスプライシングバリアントの機能解析 (2012)
  • Author(s): 野曽原志帆、原本悦和、小沼泰子、伊藤弓弦、王碧昭
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: (独)産業技術総合研究所(茨城県)
  • Year and Date: 2012-01-31
• [Presentation] 初期心臓形成に関与するヒアルロン酸経路の解析 (2012)
  • Author(s): 岩本麻未、伊藤弓弦、王碧昭
  • Organizer: 第11回産総研・産技連LS-BT合同研究発表会
  • Place of Presentation: (独)産業技術総合研究所(茨城県)
  • Year and Date: 2012-01-31
• [Presentation] 初期心臓形成に関与するヒアルロン酸シグナル経路の解析 (2012)
  • Author(s): 岩本麻未、伊藤弓弦、王碧昭
  • Organizer: テクノロジーショーケースinつくば2012
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2012-01-13
• [Presentation] Global gene expression analysis of Xenopus cells cultured in the International Space Station (KIBO). (2012)
  • Author(s): Yuzuru Ito
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: Hotel Belambra (Giens Peninsula, France)
• [Presentation] Global gene expression analysis of Xenopus cells cultured in a simulated microgravity environment and in the International Space Station (KIBO) (2012)
  • Author(s): Makoto Asashima
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: France
  • Invited
• [Presentation] Functional analyses of a novel insulin like factor that are required for neural development. (2012)
  • Author(s): Yoshikazu Haramoto
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: France
• [Presentation] Global gene expression analysis of Xenopus cells cultured in the International Space Station (KIBO). (2012)
  • Author(s): Yuzuru Ito
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: France
• [Presentation] Possibility of novel Nanog function in Xenopus: Nanog possesses dorsal mesoderm-­inducing ability by modulating BMP and Activin/nodal signaling in Xenopus. (2012)
  • Author(s): Aya Miyazaki
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: France
• [Presentation] A lectin-­based glycomic approach to Xenopus embryogenesis. (2012)
  • Author(s): Yasuko Onuma
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: France
• [Presentation] Analysis of the Xenopus laevis J-­strain genome by BAC end sequencing, FISH, and RNA-­sequence. (2012)
  • Author(s): Shuji Takahashi
  • Organizer: 14th International Xenopus Conference
  • Place of Presentation: France
• [Presentation] 次世代モデル動物ネッタイツメガエルとその輸送法の改善について (2012)
  • Author(s): 倉林 敦
  • Organizer: 日本爬虫類両棲類学会第51回大会
  • Place of Presentation: 豊田
• [Presentation] アフリカツメガエル２次胚形成における遺伝子発現ーその２ (2012)
  • Author(s): 佐藤 英二
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] ノンコーディング RNA NEAT1 の Xenopus オーソログ (2012)
  • Author(s): 佐々木保典
  • Organizer: 第14 回日本 RNA 学会年会
  • Place of Presentation: 仙台
• [Presentation] Isolation and characterization of sexually dimorphic genes during early gonadogenesis in Xenopus laevis (2011)
  • Author(s): Shuji Mawaribuchi, Nozomi Ikeda, Kazuko Fujitani, Asako Otomo, Kei Tamura, Mikako Wada, Yosuke Kamata, Yuzuru Ito, Yasuko Onuma, Mihcihiko Ito, Nobuhiko Takamatsu
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] ネッタイツメガエルの研究基盤整備 (2011)
  • Author(s): 矢尾板芳郎、浅島誠、高瀬稔、高橋秀治、伊藤弓弦
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-13
• [Presentation] ヒトiPS細胞を用いた無血清・無フィーダー培養による心筋誘導系の確立 (2011)
  • Author(s): 小池怜、伊藤弓弦、吉満亮介、丸山篤史、大沼清
  • Organizer: 第33回日本バイオマテリアル学会
  • Place of Presentation: 京都府民総合交流プラザ(京都府)
  • Year and Date: 2011-11-21
• [Presentation] アフリカツメガエル2次胚形成における遺伝子発現 (2011)
  • Author(s): 佐藤英二、小沼泰子、三浦直行、伊藤弓弦
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-21
• [Presentation] アフリカツメガエル2次胚形成における遺伝子発現について (2011)
  • Author(s): 佐藤英二、小沼泰子、三浦直行、伊藤弓弦
  • Organizer: 第44回日本発生生物学会(APDBN共催)
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-18
• [Presentation] iPS細胞樹立に最適な形に進化させた持続発現型RNAベクターの開発 (2011)
  • Author(s): 西村健、大高真奈美、高安聡子、小林俊寛、小沼泰子、伊藤弓弦、大津真、中内啓光、浅島誠、中西真人
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] 高密度レクチンアレイの開発と細胞グライコミクスへの応用 (2010)
  • Author(s): 舘野浩章、豊田雅士、小沼泰子、伊藤弓弦、斉藤秀、阿久津英憲、梅澤明弘、堀本勝久、浅島誠、平林淳
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 水溶性官能基を有する蛍光性アゾベンゼンの合成と蛍光生体染色への応用 (2010)
  • Author(s): 糸井裕亮、吉野惇郎、神戸徹也、狩野直和、川島隆幸、伊藤弓弦、浅島誠
  • Organizer: 第37回有機典型元素化学討論会
  • Place of Presentation: 室蘭
  • Year and Date: 2010-11-25
• [Presentation] Challenge the establishment of new fundamental technology in the regenerative medicine (2010)
  • Author(s): Ito, Y.
  • Organizer: 2nd Symposium on Systems and Synthetic Biology (TriSys)
  • Place of Presentation: AIST (Tokyo)
  • Year and Date: 2010-10-04
• [Presentation] Challenge the establishment of new fundamental technology in the regenerative medicine. (2010)
  • Author(s): Ito, Y.
  • Organizer: 2nd Symposium on Systems and Synthetic Biology (TriSys)
  • Place of Presentation: Tokyo
  • Year and Date: 2010-10-04
• [Book] Mechanobiology During Vertebrate Organ Development, Mechanosensing Biology (2010)
  • Author(s): Asashima M, Michiue T, Ohnuma K, Nakajima Y , Ito Y
  • Publisher: Springer
• [Book] Chapter 3 Mechanobiology During Vertebrate Organ Development, Mechanosensing Biology, pp39-49 (2010)
  • Author(s): Makoto Asashima, Tatsuo Michiue, Kiyoshi Ohnuma, Yoshiro Nakajima, Yuzuru Ito
  • Total Pages: 11
  • Publisher: Springer