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Analysis of gene expression in the cells isolated by laser capture microdissection from different petal tissues

Research Project

Project/Area Number 22780030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionKyoto Prefectural University

Principal Investigator

HARADA Taro  京都府立大学, 大学院・生命環境科学研究科, 共同研究員 (80468256)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords花卉 / カーネーション / 開花 / 遺伝子発現 / 遺伝子 / 園芸学 / 花き / マクロアレイ
Research Abstract

Cells from epidermis, parenchyma and vascular bundles were isolated from carnation petals by laser capture microdissection and differential gene expression among these petal tissues was demonstrated. In addition, differential gene expression between adaxial and abaxial sides of parenchyma in a bending zone of carnation petals was revealed by macroarray analysis. One gene encoding lipid transfer protein(DcLTP1) showed relatively high expression level in blade epidermis, while its expression was higher in the adaxial side of parenchyma from the bending zone.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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