Unraveling of interaction between Magnaporthe oryzae effectors and host targets during rice blast infection.

• Project/Area Number: 22780040
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: SAITOH Hiromasa 公益財団法人岩手生物工学研究センター, 生命科学研究部, 主任研究員 (20414336)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 非病原力エフェクタ－ / 病原性分泌タンパク質 / いもち病抵抗性遺伝子 / ウリ類炭疽病菌 / 直接的タンパク質相互作用 / 分泌タンパク質 / 細胞間隙 / 抵抗性タンパク質遺伝子 / 抵抗性誘導 / 非病原力エフェクター / 酵母2ハイブリッド法 / 非病原力エフェクター遺伝子 / ベンサミアーナタバコ / 病原力遺伝子 / 非病原力遺伝子 / 矮化症状 / 形質転換イネ懸濁培養細胞

Research Abstract

We have successfully identified three avirulence effectors AVR-Pia, AVR-Pii, AVR-Pik and a pathogenicity protein MC69 from the rice blast fungus Magnaporthe oryzae. In addition, a rice blast resistance (R) gene Pia has been isolated. We have then found out that MC69 is a secreted protein and required for infection by monocot and dicot fungal pathogens, M. oryzae and cucumber anthracnose fungus Colletotrichum orbiculare, respectively. We also revealed that an R protein Pik1 recognizes AVR-Pik through direct protein-protein interaction. Furthermore, another R gene Pii has been successfully isolated.

Research Products

• [Journal Article] MoST1 encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae (2014)
  • Author(s): Saitoh H, Hirabuchi A, Fujisawa S, Mitsuoka C, Terauchi R, Takano Y
  • Journal Title: FEMS Microbiology Letters Volume: 352 Pages: 104-113
• [Journal Article] Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice (2014)
  • Author(s): Kanzaki H, Yoshida K, Saitoh H, Tamiru M, Terauchi R
  • Journal Title: Methods in Molecular Biology Volume: 1127 Pages: 269-275
• [Journal Article] MoST1 encoding a hexose transporter-like protein is involved in both conidiation and mycelial melanization of Magnaporthe oryzae. (2014)
  • Author(s): Saitoh, H., HIrabuchi, A., Fujisawa, S., Mitsuoka, C., Terauchi, R. Takano, Y.
  • Journal Title: FEMS Microbiology Letters Volume: 352 Issue: 1 Pages: 104-113
  • DOI: 10.1111/1574-6968.12369
  • Peer Reviewed
• [Journal Article] Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice. (2014)
  • Author(s): Kanzaki, H., Yoshida, K., Saitoh, H., Tamiru, M., Terauchi, R.
  • Journal Title: Methods in molecular biology Volume: 1127 Pages: 269-275
  • DOI: 10.1007/978-1-62703-986-4_20
  • ISBN: 9781627039857, 9781627039864
  • Peer Reviewed
• [Journal Article] Deployment of the Burkholderia glumae type III secretion system as an efficient tool for translocating pathogen effectors to monocot cells (2013)
  • Author(s): Sharma S, Sharma S, Hirabuchi A, Yoshida K, Fujisaki K, Ito A, Uemura A, Terauchi R, Kamoun S, Sohn KH, Jones JDG, Saitoh H
  • Journal Title: The Plant Journal Volume: 74 Pages: 701-712
• [Journal Article] MutMap-Gap : whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii (2013)
  • Author(s): Takagi H, Uemura A, Yaegashi H, Tamiru M, Abe A, Mitsuoka C, Utsushi H, Natsume S, Kanzaki H, Matsumura H, Saitoh H, et al
  • Journal Title: New Phytologist Volume: 200 Pages: 276-283
• [Journal Article] Two distinct secretion systems facilitate tissue invasion by the rice blast fungus Magnaporthe oryzae (2013)
  • Author(s): Girald MC, Dagdas YF, Gupta YK, Mentlak TA, Yi M, Martinez-Rocha AL, Saitoh H, et al
  • Journal Title: Nature Communications Volume: 4 Pages: 1996-1996
• [Journal Article] MutMap+ : Genetic mapping and mutant identification without crossing in rice (2013)
  • Author(s): Fekih R, Takagi H, Tamiru M, Abe A, Natsume S, Yaegashi H, Sharma S, Sharma S, Kanzaki H, Matsumura H, Saitoh H, et al
  • Journal Title: PLoS One Volume: 8
• [Journal Article] Two distinct secretion systems facilitate tissue invasion by the rice blast fungus Magnaporthe oryzae. (2013)
  • Author(s): Giraldo, M.C., Dagdas, Y.F., Gupta, Y.K., Mentlak, T.A., Yi, M., Martinez-Rocha, A.L., Saitoh, H., Terauchi, R., Talbot, N.J., Valent, B.
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1996-1996
  • DOI: 10.1038/ncomms2996
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Shunichi Kosugi, Satoshi Natsume, Kentaro Yoshida, Daniel MacLean, Liliana Cano, Sophien Kamoun, and Ryohei Terauchi
  • Journal Title: PLoS One Volume: 8 Issue: 10 Pages: e75402-e75402
  • DOI: 10.1371/journal.pone.0075402
  • Peer Reviewed
• [Journal Article] Deployment of the Burkholderia glumae type III secretion system as an efficient tool for translocating pathogen effectors to monocot cells. (2013)
  • Author(s): Sharma S, Sharma S, Hirabuchi A, Yoshida K, Fujisaki K, Ito A, Uemura A, Terauchi R, Kamoun S, Sohn KH, Jones JDG, Saitoh H.
  • Journal Title: The Plant Journal Volume: 74 Issue: 4 Pages: 701-712
  • DOI: 10.1111/tpj.12148
  • Peer Reviewed
• [Journal Article] Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a secreted protein required for infection by monocot and dicot fungal pathogens (2012)
  • Author(s): Saitoh H, Fujisawa S, Mitsuoka C, Ito A, Hirabuchi A, Ikeda K, et al
  • Journal Title: PLoS Pathogens Volume: 8
• [Journal Article] Effector-mediated suppression of chitin-triggered immunity by Magnaporthe oryzae is necessary for rice blast disease (2012)
  • Author(s): Mentlak TA, Kombrink A, Shibuya T, Ryder LS, Otomo I, Saitoh H, et al
  • Journal Title: The Plant Cell Volume: 24 Pages: 322-335
• [Journal Article] Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions (2012)
  • Author(s): Kanzaki H, Yoshida K, Saitoh H, et al
  • Journal Title: The Plant Journal Volume: 72 Pages: 894-907
• [Journal Article] Mutation in OsLMS, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (Oryza sativa L.) (2012)
  • Author(s): Undan JR, Tamiru M, Abe A, Yoshida K, Kosugi S, Takagi H, Yoshida K, Kanzaki H, Saitoh H, et al
  • Journal Title: Genes & Genetic Systems Volume: 87 Pages: 169-179
  • NAID: 10030945208
• [Journal Article] Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions. (2012)
  • Author(s): Kanzaki H, Yoshida K, Saitoh H, Fujisaki K, Hirabuchi A, Alaux L, Fournier E, Tharreau D, Terauchi R.
  • Journal Title: The Plant Journal Volume: 72 Issue: 6 Pages: 894-907
  • DOI: 10.1111/j.1365-313x.2012.05110.x
  • Peer Reviewed
• [Journal Article] Mutation in <i>OsLMS</i>, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (<i>Oryza sativa</i> L.) (2012)
  • Author(s): Undan JR, Tamiru M, Abe A, Yoshida K, Kosugi S, Takagi H, Yoshida K, Kanzaki H, Saitoh H, Fekih R, Sharma S, Undan J, Yano M, Terauchi R.
  • Journal Title: Genes & Genetic Systems Volume: 87 Issue: 3 Pages: 169-179
  • DOI: 10.1266/ggs.87.169
  • NAID: 10030945208
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] Effector-mediated suppression of chitin-triggered immunity by Magnaporthe oryzae is necessary for rice blast disease (2012)
  • Author(s): T.A.Mentlak, A.Kombrink, T.Shinya, L.S.Ryder, I.Otomo, H.Saitoh, R.Terauchi, Y.Nishizawa, N.Shibuya, B.P.H.J.Thomma, N.J.Talbot
  • Journal Title: Plant Cell Volume: 24 Issue: 1 Pages: 322-335
  • DOI: 10.1105/tpc.111.092957
  • Peer Reviewed
• [Journal Article] A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes (2011)
  • Author(s): Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, et al
  • Journal Title: The Plant Journal Volume: 66 Pages: 467-479
• [Journal Article] Studying of genome-wide DNA polymorphisms to understand Magnaporthe-rice interactions (2011)
  • Author(s): Terauchi R, Yoshida K, Saitoh H, et al
  • Journal Title: Australasian Plant Pathology Volume: 40 Pages: 328-334
• [Journal Article] A multifaceted genomica approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes. (2011)
  • Author(s): Okuyama Y, Kanzaki H, Abe A, Yoshida K, Tamiru M, Saitoh H, et al.
  • Journal Title: The Plant Journal Volume: 66巻 Pages: 467-479
  • Peer Reviewed
• [Presentation] イネいもち病菌AVR-Pik、AVR-Piiの分泌・宿主細胞内移行の解明 (2014)
  • Author(s): 齋藤宏昌、吉田健太郎、植村亜衣子、藤崎恒喜、小林光智衣、神崎洋之、寺内良平
  • Organizer: 平成２６年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] Analysis of secretion of AVR-Pik, AVR-Pii from Magnaporthe oryzae and translocation of the AVRs into rice cells. (2014)
  • Author(s): Saitoh, H., Yoshida, K., Uemura, A., Fujisaki, K., Kobayashi, M., Kanzaki, H., Terauchi, R.
  • Organizer: XVI International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Rodos Palace hotel, Rhodes island, Greece
• [Presentation] イネいもち病菌の胞子生産と菌糸のメラニン化に関与するヘキソーストランスポーター様タンパク質遺伝子MoST1 (2013)
  • Author(s): 齋藤宏昌, 高野義孝, 寺内良平
  • Organizer: 平成25年度日本植物病理学会東北部会
  • Place of Presentation: 秋田市にぎわい交流館
  • Year and Date: 2013-10-28
• [Presentation] イネもみ枯細菌病菌III型分泌機構を利用したイネいもち病菌エフェクタ-の単子葉植物細胞内への移行 (2013)
  • Author(s): 齋藤宏昌, Shailendra Sharma ら
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学
  • Year and Date: 2013-03-28
• [Presentation] Deployment of the Burkholderia glumae type III secretion system as an efficient tool for studying Magnaporthe oryzae effector functions in plant cells (2013)
  • Author(s): 齋 藤宏昌, Shailendra Sharma ら
  • Organizer: The 6th International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea
• [Presentation] Deployment of the Burkholderia glumae Type III secretion system as an efficient tool for studying Magnaporthe oryzae effector functions in plant cells. (2013)
  • Author(s): Saitoh, H., Sharma, S., Hirabuchi, A., Sharma, S., Yoshida, K., Fujisaki, K., Uemura, A., Kamoun, S., Sohn, K.H., Jones, J.D.G., Terauchi, R.
  • Organizer: The 6th International Rice Blast Conference
  • Place of Presentation: Ramada Plaza Jeju Hotel, Jejudo, South Korea
• [Presentation] イネいもち病菌の胞子生産と菌糸のメラニン化に関与するヘキソーストランスポーター様タンパク質遺伝子MoST1 (2013)
  • Author(s): 齋藤宏昌、高野義孝、寺内良平
  • Organizer: 平成２５年度日本植物病理学会東北部会
  • Place of Presentation: 秋田市にぎわい交流館
• [Presentation] イネもみ枯細菌病菌III型分泌機構を利用したイネいもち病菌エフェクタ－の単子葉植物細胞内への移行 (2013)
  • Author(s): 齋藤宏昌
  • Organizer: 平成２５年度日本植物病理学会大会
  • Place of Presentation: 岐阜大学
• [Presentation] イネいもち病菌の大規模遺伝子破壊解析によって同定された分泌タンパク質MC69は単子葉および双子葉病原菌による感染に必要とされる (2012)
  • Author(s): 齋藤宏昌, 吉田健太郎, 池田恭子ら
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-03-29
• [Presentation] Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a secreted protein required for infection by monocot and dicot fungal pathogens (2012)
  • Author(s): 齋藤宏昌, 三岡周子, 平渕亜紀子ら
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: 国立京都国際会館
• [Presentation] Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a secreted protein required for infection by monocot and dicot fungal pathogens (2012)
  • Author(s): 齋藤宏昌, 三岡周子, 平渕亜紀子ら
  • Organizer: 30th New Phytologist Symposium
  • Place of Presentation: Stanford Sierra Conference Center, California, USA
• [Presentation] イネいもち病菌病原力エフェクタ-MC69の機能解析 (2010)
  • Author(s): 齋藤宏昌, 藤澤志津子, 伊東明子, 寺内良平
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-04-19
• [Presentation] イネいもち病菌病原力エフェクターMC69の機能解析 (2010)
  • Author(s): 齋藤宏昌、藤澤志津子、伊東明子、寺内良平
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-04-19
• [Remarks] 岩手生物工学研究センター
  • URL: https://sites.google.com/a/ibrc.or.jp/ibrc/
• [Remarks] 岩手生物工学研究センター
  • URL: https://sites.google.com/a/ibrc.or.jp/ibrc/
• [Remarks] 岩手生物工学研究センターホームページ
  • URL: http://genome.ibrc.or.jp/
• [Remarks] 新学術領域｜ゲノム・遺伝子相関【岩手生工研】寺内班アーカイブ
  • URL: http://www.ige.tohoku.ac.jp/prg/genetics/study_report/terauchi/