The role of serine protease granzyme on the induction of itch in atopic dermatitis

• Project/Area Number: 22790063
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: University of Toyama
• Principal Investigator: ANDOH Tsugunobu 富山大学, 大学院・医学薬学研究部(薬学), 准教授 (50333498)
• Research Collaborator: KIMURA Yusuke 富山大学, 薬学部薬学科, 学生
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 痒み / アトピー性皮膚炎 / プロテアーゼ / グランザイム / 皮膚 / NCマウス / T細胞 / 掻き動作 / プロテアーゼ活性化受容体

Research Abstract

The precious mechanisms of itch in atopic dermatitis are unclear. Since T cells infiltrate into the skin with atopic dermatitis and the proteases are also involved in the severe itch, we focused proteases produced by T cells for demonstrating the itch mechanisms in the dermatitis. It was demonstrated that granzyme A, which is one of 11 subtypes of granzymes, elicited itch through the activation of proteinase-activated receptor 2

Research Products

• [Journal Article] Involvement of serine protease and proteinase-activated receptor 2 in dermatophyte-associated itch in mice (2012)
  • Author(s): Tsugunobu Andoh、Yusuke Takayama、Takako Yamakoshi、他4名
  • Journal Title: Journal of Pharmacology and Experimental Therapeutics Volume: 343巻 Issue: 1 Pages: 91-96
  • DOI: 10.1124/jpet.112.195222
  • Peer Reviewed
• [Journal Article] Cathepsin E induces itch-related response through the production of endothelin-1 in mice (2012)
  • Author(s): Tsugunobu Andoh
  • Journal Title: European Journal of Pharmacology Volume: 686 Issue: 1-3 Pages: 16-21
  • DOI: 10.1016/j.ejphar.2012.04.024
  • Peer Reviewed
• [Journal Article] Gastrin-releasing peptide induces itch-related responses through mast cell degranulation in mice (2011)
  • Author(s): Tsugunobu Andoh、Takashi Kuwazono、他2名
  • Journal Title: Peptides Volume: 32巻 Issue: 10 Pages: 2098-20103
  • DOI: 10.1016/j.peptides.2011.09.003
  • Peer Reviewed
• [Journal Article] Involvement of leukotriene B4 in spontaneous itch-related behavior in NC mice with atopic dermatitis-like skin lesions (2011)
  • Author(s): Tsugunobu Andoh、Satomi Haza、他2名
  • Journal Title: Experimental Dermatology Volume: 20巻 Issue: 11 Pages: 894-898
  • DOI: 10.1111/j.1600-0625.2011.01346.x
  • Peer Reviewed
• [Journal Article] Noradrenergic regulation of itch transmission in the spinal cord mediated by α-adrenoceptors (2011)
  • Author(s): Yoshikazu Gotoh
  • Journal Title: Neuropharmacology Volume: 61 Issue: 4 Pages: 825-831
  • DOI: 10.1016/j.neuropharm.2011.05.030
  • Peer Reviewed
• [Journal Article] Tonic inhibition of allergic itch signaling by the descending noradrenergic system in mice. (2011)
  • Author(s): Yoshikazu Gotoh
  • Journal Title: Journal of Pharmacological Sciences Volume: Vol.115 Pages: 417-420
  • Peer Reviewed
• [Journal Article] Inhibitory Effects of the Methanol Extract of Ganoderma lucidum on Mosquito Allergy-Induced Itch-Associated Responses in Mice (2010)
  • Author(s): Tsugunobu Andoh、Qun Zhang、
  • Journal Title: Journal of Pharmacological Sciences Volume: 114 Issue: 3 Pages: 292-297
  • DOI: 10.1254/jphs.10180FP
  • NAID: 130000439885
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Journal Article] Inhibitory effects of methanol extract of Ganoderma lucidum on mosquito allergy-induced itch-associated responses in mice. (2010)
  • Author(s): Tsugunobu Andoh
  • Journal Title: Journal of Pharmacological Sciences Volume: Vol.114 Pages: 292-297
  • Peer Reviewed
• [Journal Article] Clonidine inhibits itch-related response through stimulation of α2-adrenoceptors in the spinal cord in mice. (2010)
  • Author(s): Yoshikazu Gotoh
  • Journal Title: European Journal of Pharmacology Volume: Vol.650 Pages: 215-219
  • Peer Reviewed
• [Presentation] どうしても止めたい「かゆみ」。でも「かゆみ」ってどうやって起こるの? (2012)
  • Author(s): 安東嗣修
  • Organizer: 平成24年度富山駅前サテライト公開講座「知の探求～豊かな暮らしのために～」
  • Place of Presentation: 富山
  • Year and Date: 2012-05-19
• [Presentation] 「どうしても止めたい「かゆみ」。でも「かゆみ」ってどうやって起こるの？」 (2012)
  • Author(s): 安東嗣修
  • Organizer: 平成24年度富山駅前サテライト公開講座 「知の探求～豊かな暮らしのために～」
  • Place of Presentation: 富山
  • Invited
• [Presentation] 「かゆみの動物モデルの作製と評価方法」 (2012)
  • Author(s): 安東嗣修
  • Organizer: 情報技術セミナー
  • Place of Presentation: 東京
  • Invited
• [Presentation] Mechanisms of itch in atopic dermatitis and the therapy using Traditional Kampo Medicine (2011)
  • Author(s): Tsugunobu Andoh
  • Organizer: Symposium in Research Institute of Korean Oriental Medicine in Sunchon National University
  • Place of Presentation: Suncheon、Korea
  • Year and Date: 2011-11-22
• [Presentation] Mechanisms of itch in atopic dermatitis and the therapy using Traditional Kampo Medicine (2011)
  • Author(s): Tsugunobu Andoh
  • Organizer: Symposium in Research Institute of Korean Onental Medicine in Sunchon National University
  • Place of Presentation: Suncheon, Korea
  • Year and Date: 2011-11-22
• [Presentation] Proteases and itch (2011)
  • Author(s): Tsugunobu Andoh、他
  • Organizer: 21st International Symposium of Itch
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-29
• [Presentation] アレルギー性掻痒と新規疾患分子マーカー (2011)
  • Author(s): 安東嗣修
  • Organizer: 富山大学コラボフェスタ2011
  • Place of Presentation: 富山
  • Year and Date: 2011-09-28
• [Presentation] Regulation of itch in atopic dermatitis using traditional medicine (2011)
  • Author(s): Tsugunobu Andoh
  • Organizer: 13th International Symposium on Traditional Medicine
  • Place of Presentation: 富山
• [Presentation] Granzyme A and proteinase-activated receptor 2 are involved in the induction of itch-associated responses to mosquito allergy in mice (2011)
  • Author(s): Tsugunobu Andoh、他
  • Organizer: the 6th World Congress on Itch、International Workshop for the Study of Itch
  • Place of Presentation: Brest、France
• [Presentation] 蚊アレルギー性掻痒へのプロテアーゼ活性化受容体2とCD4陽性T細胞由来グランザイムAの関与 (2011)
  • Author(s): 安東嗣修、他
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Granzyme A and proteinase-activated receptor 2 are involved in the induction of itch-associated responses to mosquito allergy in mice (2011)
  • Author(s): Tsugunobu Andoh
  • Organizer: The 6th World Congress on Itch, International Workshop for the Study of Itch
  • Place of Presentation: Brest, France
• [Presentation] アトピー性皮膚炎のマウスモデルの自発的掻き動作におけるロイコトリエンB4の関与 (2010)
  • Author(s): 羽座沙都美、他
  • Organizer: 日本薬学会北陸支部第122回例会
  • Place of Presentation: 金沢
  • Year and Date: 2010-11-21
• [Presentation] 脊髄のαアドレナリン受容体活性化を介したミルナシプランの抗掻痒効果 (2010)
  • Author(s): 後藤義一
  • Organizer: 第20回国際痒みシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-10-31
• [Presentation] Peripheral mechanisms of the induction of itch (2010)
  • Author(s): Tsugunobu Andoh
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-03
• [Presentation] アレルギー疾患の新規バイオマーカーグランザイムA (2010)
  • Author(s): 安東嗣修
  • Organizer: 2010バイオアカデミーフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2010-06-30
• [Presentation] 動物モデルと痒みの評価 法 (2010)
  • Author(s): 安東嗣修
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-16
• [Presentation] 動物モデルと痒みの評価法 (2010)
  • Author(s): 安東嗣修
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-16
• [Book] オピオイドと痒み:臨床医必須-全身とかゆみ,宮地良樹編 (2011)
  • Author(s): 安東嗣修,他
  • Total Pages: 191
  • Publisher: 診断と治療社
• [Book] オピオイドと痒み:臨床医必携-全身とかゆみ(宮地良樹編) (2011)
  • Author(s): 安東嗣修
  • Total Pages: 191
  • Publisher: 診断と治療社
• [Patent(Industrial Property Rights)] アレルギー性疾患のバイオマーカーおよびその利用 (2010)
  • Inventor(s): 安東嗣修、中野 祐、倉石 泰
  • Industrial Property Rights Holder: 富山大学
  • Filing Date: 2010
  • Overseas