| Project/Area Number |
22790596
|
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| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 法医鑑定学 / 法医検査 / 死後経過時間 / 生体時計 / リアルタイムPCR |
|---|
| Research Abstract |
We hypothesized that circadian rhythm-related gene mRNA expression level correlates to the time of death in order to establish the new estimation method using molecular biological techniques in forensic field. Arntl, Clock, Cry, and Per2 were chosen as target genes for realtime-PCR analysis in mice sacrificed every 6 hrs interval. Some genes showing dynamic expression change in Brain or Kidney seemed to be useful to estimate the time of death.
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