The role of Tra2βmRNA variants in cellular proliferation and apoptosis

• Project/Area Number: 22790649
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: The University of Tokushima
• Principal Investigator: KUWANO Yuki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (00563454)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 選択的スプライシング / 大腸がん / Transformer 2β / SRタンパク質 / tra2 beta / 遺伝子発現 / 消化管上皮細胞 / マイクロアレイ / transformer 2 beta

Research Abstract

In response to oxidative stress, colon cancer cells(HCT116) increased levels of TRA2β1 mRNA without changing its mRNA stability. TRA2βpromoters and Chip assay demonstrated that an Ets1-binding site was crucial for basal transcription, while three heat shock elements mediated the oxidant-induced transactivation of TRA2β. Tra2βknockdown caused apoptosis of HCT116 cells. Tra2βwere preferentially expressed in proliferative compartment of normal human colonic glands and adenocarcinomas, where Ets1 and HSF1 were also highly expressed.

Research Products

• [Journal Article] MicroRNAs miR-144/144* and miR-16 in peripheral blood are potential biomarkers for naturalistic stress in healthy Japanese medical students. (2012)
  • Author(s): Katsuura S, Kuwano Y, Yamagishi N, Kurokawa K, Kajita K, Akaike Y, Nishida K, Masuda K, Tanahashi T, Rokutan K.
  • Journal Title: Neurosci Lett. Volume: 516 Issue: 1 Pages: 79-84
  • DOI: 10.1016/j.neulet.2012.03.062
  • Peer Reviewed
• [Journal Article] General RBP expression in human tissues as a function of age (2012)
  • Author(s): Masuda K, et al.
  • Journal Title: Ageing Res Rev Volume: 11(4) Issue: 4 Pages: 423-31
  • DOI: 10.1016/j.arr.2012.01.005
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells (2012)
  • Author(s): Kurokawa K, Tanahashi T, Iima T, Yamamoto Y, Akaike Y, Nishida K, Masuda K, Kuwano Y, Murakami Y, Fukushima M, Rokutan
  • Journal Title: J Gastroenterol Volume: 47 Issue: 8 Pages: 883-895
  • DOI: 10.1007/s00535-012-0547-6
  • NAID: 10031051201
  • Peer Reviewed
• [Journal Article] Autism-associated gene expression in peripheral leucocytes commonly observed between subjects with autism and healthy women having autistic children. (2011)
  • Author(s): Kuwano Y, Kamio Y, Kawai T, Katsuura S, Inada N, Takaki A, Rokutan K.
  • Journal Title: PLoS One Volume: 6 Issue: 9 Pages: e24723-e24723
  • DOI: 10.1371/journal.pone.0024723
  • Peer Reviewed
• [Journal Article] Skipping of an alternative intron in the srsf1 3' untranslated region increases transcript stability (2011)
  • Author(s): Akaike Y, Kurokawa K, Kajita K, Kuwano Y, Masuda K, Nishida K, Kang SW, Tanahashi T, Rokutan K
  • Journal Title: The Journal of Medical Investigation Volume: 58 Issue: 3,4 Pages: 180-187
  • DOI: 10.2152/jmi.58.180
  • NAID: 130004822655
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state and expression of glucocorticoid receptor α and β isoforms in healthy Japanese medical students. (2011)
  • Author(s): Kurokawa K, Tanahashi T, Murata A, Akaike Y, Katsuura S, Nishida K, Masuda K, Kuwano Y, Kawai T, Rokutan K.
  • Journal Title: Stress Volume: 14 Issue: 4 Pages: 431-438
  • DOI: 10.3109/10253890.2011.555930
  • Peer Reviewed
• [Journal Article] Circulating vascular endothelial growth factor is independently and negatively associated with trait anxiety and depressive mood in healthy Japanese university students. (2011)
  • Author(s): Katsuura S, Kamezaki Y, Yamagishi N, Kuwano Y, Nishida K, Masuda K, Tanahashi T, Kawai T, Arisawa K, Rokutan K.
  • Journal Title: Int J Psychophysiol Volume: 81 Issue: 1 Pages: 38-43
  • DOI: 10.1016/j.ijpsycho.2011.04.004
  • Peer Reviewed
• [Journal Article] Skipping of an alternative intron in tne srsfl 3 untranslated region increases transcript stability (2011)
  • Author(s): Akaike Y, et.al.
  • Journal Title: Journal of Medical Investigation Volume: 58 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral chang (2010)
  • Author(s): Yamamoto Y, Tanahashi T, Katsuura S, Kurokawa K, Nishida K, Kuwano Y, Kawai T, Teshima-Kondo S, Chikahisa S, Tsuruo Y, Sei H, Rokutan K.
  • Journal Title: J Neuroimmunol Volume: 229 Issue: 1-2 Pages: 129-139
  • DOI: 10.1016/j.jneuroim.2010.07.024
  • Peer Reviewed
• [Journal Article] Brief naturalistic stress induces an alternative splice variant of SMG-1 lacking exon 63 in peripheral leukocyte (2010)
  • Author(s): Kurokawa K, Kuwano Y, Tominaga K, Kawai T, Katsuura S, Yamagishi N, Satake Y, Kajita K, Tanahashi T, Rokutan K
  • Journal Title: Neurosci Lett Volume: 484 Issue: 2 Pages: 128-132
  • DOI: 10.1016/j.neulet.2010.08.031
  • Peer Reviewed
• [Journal Article] Brief naturalistic stress induces an alternative splice variant of SMG-1 lacking exon 63 in peripheral leukocytes. (2010)
  • Author(s): Kurokawa K, et.al
  • Journal Title: Neurosci Lett. Volume: 484 Pages: 128-32
  • Peer Reviewed
• [Presentation] ストレス応答性Tra2b4 PTCバリアントによるp21の転写調節を介した細胞老化の制御 (2011)
  • Author(s): 梶田敬介、桑野由紀、山岸直子、黒川憲、佐竹譲、赤池瑶子、本田真奈美、増田清士、棚橋俊仁、六反一仁.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-16
• [Presentation] Hu antigen R(HuR) Functions as An Alternative Pre-mRNA Splicing Regulator of Transformer 2-beta(Tra2beta) under Oxidative Stress(酸化ストレス下での、HuRによるtransformer 2-beta(Tra2b)の選択的スプライシング制御機構) (2011)
  • Author(s): 赤池瑶子、増田清士、山岸直子、黒川憲、佐竹譲、梶田敬介、本田真奈美、西田憲生、桑野由紀、棚橋俊仁、六反一仁
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-16
• [Presentation] ストレス応答性Tra2beta4 PTCバリアントによるp21の転写調節を介した細胞老化の制御 (2011)
  • Author(s): 梶田敬介
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-16
• [Presentation] Autism-associated gene expression signatures in peripheral blood leucocytes (2011)
  • Author(s): 桑野由紀、神尾陽子、河合智子、勝浦桜子、稲田尚子、高木晶子、六反一仁
  • Organizer: Joint Academic Conference on ASD 2011
  • Place of Presentation: 日本財団ビル(東京)
  • Year and Date: 2011-12-02
• [Presentation] 遺伝子発現からみたストレス脆弱性,シンポジウム講演 (2011)
  • Author(s): 桑野由紀
  • Organizer: 第27回日本ストレス学会学術総会
  • Place of Presentation: 東京国際交流館(東京)
  • Year and Date: 2011-11-20
• [Presentation] 精神的ストレスに応答するヒト末梢血マイクロRNAの発現 (2011)
  • Author(s): 本田真奈美、桑野由紀、山岸直子、黒川憲、佐竹譲、梶田敬介、赤池瑶子、西田憲生、増田清士、棚橋俊仁、六反一仁
  • Organizer: 第27回日本ストレス学会学術総会
  • Place of Presentation: 東京国際交流館(東京)
  • Year and Date: 2011-11-20
• [Presentation] 遺伝子発現から見たストレス脆弱性 (2011)
  • Author(s): 桑野由紀
  • Organizer: 第27回日本ストレス学会学術総会
  • Place of Presentation: 東京国際交流館(東京都)(招待講演)
  • Year and Date: 2011-11-20
• [Presentation] Transfomer-2beta regulates apoptosis through post-transcriptional regulation of bcl-2 (2011)
  • Author(s): Kuwano Y, Kajita K, Satake Y, Kurokawa K, Yamagishi N, Akaike Y, Honda M, Nishida K, Masuda K, Tanahashi T, Rokutan K
  • Organizer: Cell Symposia Regulatory RNAs
  • Place of Presentation: Wyndham hotel(Chicago, IL, U. S. A)
  • Year and Date: 2011-10-11
• [Presentation] Transfomer-2beta regulates apoptosis through post-transcriptional regulation of bc1-2 (2011)
  • Author(s): Y.Kuwano
  • Organizer: Cell Symposia Regulatory RNAs
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-10-10
• [Presentation] Premature stop codon-containing tra2beta4 mRNA isoform regulates cellular senescence (2011)
  • Author(s): Kajita K, Kuwano Y, Masuda K, Yamagishi N, Kurokawa K, Satake Y, Akaike Y, Honda M, Tanahashi T, Rokutan K
  • Organizer: 3rd EMBO Meeting 2011
  • Place of Presentation: Austria Center Vienna(Vienna, Austria)
  • Year and Date: 2011-09-12
• [Presentation] Premature stop codon-containing tra2beta4 mRNA isoform regulates cellular senescence (2011)
  • Author(s): K.Kajita
  • Organizer: 3rd EMBO Meeting 2011
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-09-10
• [Presentation] 酸化ストレス応答性Tra2b4 mRNAによる細胞老化の制御 (2011)
  • Author(s): 梶田敬介、桑野由紀、山岸直子、黒川憲、佐竹譲、赤池瑶子、本田真奈美、西田憲生、増田清士、棚橋俊仁、六反一仁
  • Organizer: 第6回臨床ストレス応答学会大会
  • Place of Presentation: 名古屋大学(名古屋市)
• [Presentation] 大腸がん由来細胞株におけるTransfomer 2 βの酸化ストレス応答の解明 (2010)
  • Author(s): 桑野由紀
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-08
• [Presentation] Alternative splice variants of SMG-1 as a potential marker for brief naturalistic stressors in peripheral leukocytes. (2010)
  • Author(s): 桑野由紀
  • Organizer: The 7^<th> world congress on stress
  • Place of Presentation: ライデン(オランダ)
  • Year and Date: 2010-08-25
• [Presentation] The mechanism of transfomer 2-β gene expression in response to oxidative stress in human colon epithelial cells (2010)
  • Author(s): 桑野由紀
  • Organizer: Digestive Disease Week 2010米国消化器病学会
  • Place of Presentation: ニューオーリンズ(アメリカ)
  • Year and Date: 2010-05-02
• [Presentation] 大腸がん由来細胞株におけるTransformer 2-betaの酸化ストレス応答の解明 (2010)
  • Author(s): 桑野由紀, 佐竹譲, 北村奈瑠香, 梶田敬介, 黒川憲, 山岸直子, 勝浦桜子, 棚橋俊仁, 近藤茂忠, 六反一仁
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(神戸)
• [Presentation] ヒトtransformer 2 beta遺伝子における選択的スプライシング制御機構の解明 (2010)
  • Author(s): 佐竹譲、桑野由紀、梶田敬介、黒川憲、山岸直子、勝浦桜子、北村奈瑠香、増田清士、棚橋俊仁、六反一仁
  • Organizer: 第5回臨床ストレス応答学会大会
  • Place of Presentation: 徳島大学(徳島)
• [Presentation] Alternative splice variants of SMG-1 as a potential marker for brief naturalistic stressors in peripheral leukocytes (2010)
  • Author(s): Yuki Kuwano, Ken Kurokawa, Sakurako Katsuura, Naoko Yamagishi, Yuzuru Satake, Keisuke Kajita, Toshihito Tanahashi, Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden University(Leiden), Netherlands
• [Presentation] The mechanism of transfomer 2-beta gene expression in response to oxidative stress in human colon epithelial cells (2010)
  • Author(s): Yuki Kuwnao, Yuzuru Satake, Kensei Nishida, Kiyoshi Masuda, Ken Kurokawa, Shigetada Teshima-kondo, Toshihito Tanahashi, Kazuhito Rokutan
  • Organizer: Digestive Disease Week 2010
  • Place of Presentation: Ernest N Morial Convention Center(New Orleans, LA, USA)