Evaluating Plasma Endothelial Cell-derived Micro particles for Detecting Abnormal Expression of Molecules Involved in Onset of Pulmonary Arterial Hypertension Associated with Connective Tissue Diseases

• Project/Area Number: 22790687
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: MARUYAMA Hidekazu 筑波大学, 附属病院, 病院講師 (30528493)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 肺高血圧症 / 血管内皮細胞 / バイオマー Jー / マイクロパーティクル / 膜タンパク / 膠原病 / 接触阻害 / セロトニン / バイオマーカー / miRNA

Research Abstract

We aimed to establish a new method for diagnosing pulmonary arterial hypertension. The endothelial cell-derived microparteicles in plasma are quantified targeting six surface antigens, although, we could not find significant change between the patients and controls. To select new surface markers, we created an in vitro model using cultivated endothelial cells evaluated by impairment of contact inhibition. Certain two factors, which are thought to be related to onset of the disease, were essential for this impairment.