Elucidation of physiological function and mechanism of DA-Raf in cardiac hypertrophy and heart failure
| Project/Area Number |
22790688
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Chiba University |
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Principal Investigator |
TAKANO Haruko 千葉大学, バイオメディカル研究センター, 機関研究員 (40532891)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分子心臓病態学 / Ras-ERKカスケード / Rafファミリー / 肺胞形成 / II型肺胞上皮細胞 / 筋線維芽細胞 / 上皮間充織転換 / ノックアウトマウス / 発生・分化 / シグナル伝達 / 循環器・高血圧 / 細胞・組織 / 心肥大 |
|---|
| Research Abstract |
We have identified DA-Raf (deleted A-Raf), which is generated by alternative splicing from Araf gene. It contains the Ras-binding domain, but lacks the kinase domain. To elucidate physiological functions of DA-Raf and their mechanisms, we generated DAraf gene-deficient (KO) mice, in which Araf expression is not affected. Lung alveoli were poorly developed due to the lack of myofibroblasts in the KO mice. On the other hand, alveolar epithelial type 2 cells (AEC2), which expressed DA-Raf at high level, might transform by epithelial-mesenchymal transition to myofibroblasts. These results imply that DA-Raf plays essential roles in alveolarization by regulating the Ras-ERK pathway in AEC2-myofibrblast transition.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma2012
Author(s)
Kinoshita T, Nohata N, Watanabe-Takano H, Yoshino H, Hidaka H, Fujimura L, Fuse M, Yamasaki T, Enokida H, Nakagawa M, Hanazawa T, Okamoto Y, Seki N.
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Journal Title
Int. J. Oncol
Volume: (印刷中)
Pages: 1770-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Tumor suppressive microRNA-133a regulates novel targets : Moesin contributes to cancer cell proliferationand invasion in head and neck squamous cell carcinoma2012
Author(s)
Kinoshita T, Nohata N, Fuse M, Hanazawa T, Kikkawa N, Fujimura L, Watanabe-Takano H, Yamada Y, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 418(2)
Pages: 378-383
Related Report
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[Journal Article] Tumor suppressive microRNA-133a regulates novel targets : Moesin contributes to cancer cell proliferation and invasion in head and neck squamous cell carcinoma2012
Author(s)
Kinoshita T, Nohata N, Fuse M, Hanazawa T, Kikkawa N, Fujimura L, Watanabe-Takano H, Yamada Y, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 418
Pages: 378-382
Related Report
Peer Reviewed
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[Book] 実験医学2011
Author(s)
高野和儀, 渡邊-高野晴子, 遠藤剛
Total Pages
3
Publisher
羊土社
Related Report
