The pathogenesis induced by highly pathogenic avian influenza virus H5N1 hemagglutinin

• Project/Area Number: 22790953
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Infectious disease medicine
• Research Institution: Osaka University
• Principal Investigator: DAIDOJI Tomo 大阪大学, 微生物病研究所, 特任助教 (80432433)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 高病原性鳥インフルエンザ / 高病原性インフルエンザウイルス / アポトーシス

Research Abstract

In recent years, the highly pathogenic avian influenza virus H5N1 has raised serious worldwide concern about an influenza pandemic ; however, the mechanism of H5N1 pathogenesis is largely unknown. A/ Crow/ Kyoto/ 53/ 2004(H5N1)[ Cr/ Ky(H5N1)] showed high mortality and weight loss toward mice, while A/ Duck/ Hong Kong/ 820/ 80(H5N3) did not. In addition, recombinant H5N3 viruses carrying the H5N1 HA gene(rH5N3H5N1HA) showed the similar result with that of Cr/ Ky(H5N1). In addition, Cr/ Ky(H5N1) and rH5N3H5N1HA also indicated efficient viral replication and lung hemorrhage including inflammatory cell infiltration. The levels of proinflammatory cytokine in mice infected with rH5N3H5N1HA was higher than mice infected with Dk/ Hk(H5N3). Taken together, these data suggest that H5N1HA broaden the viral pathogenesis in mice and this pathogenesis is based on viral replication and proinflammatory cytokine balance in mice.

Research Products

• [Journal Article] Frequency of D222G and Q223R hemagglutinin mutants of pandemic(H1N1) 2009 influenza virus in Japan between 2009 and 2010 (2012)
  • Author(s): Yasugi M, Nakamura S, Daidoji T, Kawashita N, Ramadhany R, Yang CS, Yasunaga T, Iida T, Horii T, Ikuta K, Takahashi K, Nakaya T
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010 (2012)
  • Author(s): Yasugi M, Nakamura S, Daidoji T, Kawashita N, Ramadhany R, Yang CS, Yasunaga T, Iida T, Horii T, Ikuta K, Takahashi K, Nakaya T
  • Journal Title: PLoS One Volume: 7 Issue: 2 Pages: e30946-e30946
  • DOI: 10.1371/journal.pone.0030946
  • Peer Reviewed
• [Journal Article] Novel type II transmembrane serine proteases, MSPL and TMPRSS13 (2010)
  • Author(s): Okumura Y, Takahashi E, Yano M, Ohuchi M, Daidoji T, Nakaya T, Bottcher E, Garten W, Klenk HD, Kido H
  • Journal Title: Proteolytically activate membrane fusion activity of the hemagglutinin of highly pathogenic avian influenza viruses and induce their multicycle replication Volume: 84 Pages: 5089-5096
  • Peer Reviewed
• [Journal Article] The Role of Apoptosis in Influenza Virus Pathogenesis and the Mechanisms Involved in Anti-Influenza Therapies (2010)
  • Author(s): Daidoji T, Kaihatsu K, Nakaya T
  • Journal Title: Curr Chem Biol. Volume: 4 Pages: 208-218
  • Peer Reviewed
• [Journal Article] Novel type II transmembrane serine proteases, MSPL and TMPRSS13, Proteolytically activate membrane fusion activity of the hemagglutinin of highly pathogenic avian influenza viruses and induce their multicycle replication. (2010)
  • Author(s): Okumura Y, Takahashi E, Yano M, Ohuchi M, Daidoji T, Nakaya T, Bottcher E, Garten W, Klenk HD, Kido H
  • Journal Title: J Virol Volume: 84 Pages: 5089-5096
  • Peer Reviewed
• [Journal Article] The Role of Apoptosis in Influenza Virus Pathogenesis and the Mechanisms Involved in Anti-Influenza Therapies (2010)
  • Author(s): Daidoji T, Kaihatsu K, Nakaya T
  • Journal Title: Curr Chem Biol Volume: 4 Pages: 208-218
  • Peer Reviewed
• [Presentation] WIDE-RANGED CELL TROPISM OF ASIAN-H5N1 VIRUSES IN HUMAN AIRWAY EPITHELIAL CELLS (2011)
  • Author(s): Tomo Daidoji, Madiha S Ibrahim, Yohei Watanabe, Mayo Yasugi, Cheng-Song Yang, Kazuyoshi Ikuta, Takaaki Nakaya
  • Organizer: IUMS 2011 Sapporo
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-10-15
• [Presentation] WIDE-RANGED CELL TROPISM OF ASIAN-H5N1 VIRUSES IN HUMAN AIRWAY EPITHELIAL CELLS (2011)
  • Author(s): Tomo Daidoji
  • Organizer: IUMS 2011 Sapporo
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2011-09-15
• [Presentation] Wide-ranged cell tropism of Asian-H5N1 viruses in human airway epithelial cells (2010)
  • Author(s): Tomo Daidoji, Madiha S Ibrahim, Yohei Watanabe, Mayo Ueda, Kazuyoshi Ikuta, Takaaki Nakaya
  • Organizer: Cell Symposia : Influenza Conference
  • Place of Presentation: Washington Marriot Hotel, Washington DC, USA
  • Year and Date: 2010-12-03
• [Presentation] 高病原性鳥インフルエンザウイルスH5N1はヒト呼吸器上皮細胞に対して広いトロピズムを示す (2010)
  • Author(s): 大道寺智、Ibrahim Madiha S、渡邊洋平、上田真世、楊成松、生田和良
  • Organizer: 第58回ウイルス学会学術集会
  • Place of Presentation: あわぎんホール
  • Year and Date: 2010-11-08