Investigation of the mechanism of metabotropic glutamate receptor 5 activity which protein related to psychiatric disorder.

Research Project

Project/Area Number	22791149
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Psychiatric science
Research Institution	Kurume University
Principal Investigator	UEMATSU Ken 久留米大学, 医学部, 助教 (60441672)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	精神薬理学 / 分子生物学 / 細胞内情報伝達 / 蛋白リン酸化修飾 / 細胞内カルシウム動態 / 統合失調症 / 代謝型グルタミン酸受容体5 / プロテインキナーゼA / 細胞内カルシウム / 神経科学
Research Abstract	In this research, we discovered new phosphorylation site of mGluR5, which phosphorylated by PKA and the site is serine 870 of mGluR5. Methods of this study, we generate mGluR5 C-teerminal peptie for in vitro phosphorylation assay by protein kinase A and【γ-32P】ATP. Compared with wild and mutant mGluR5 C-terminal peptide, mutant inhibited to gain【γ-32P】labeled isotope. Now we set up the experiment of the function by this mGluR5 phosphorylation.