Lipid analysis of hepatocellular carcinoma using imaging mass spectrometry
| Project/Area Number |
22791271
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肝細胞癌 / 質量顕微鏡 / リン脂質 / リモデリング酵素 / LPCAT1 / 質量顕微鏡法 / リン脂質リモデリング / LPCAT / 細胞増殖 / 浸潤 / siRNA |
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| Research Abstract |
Molecular lipidomic screening was performed by imaging mass spectrometry (IMS) in 37 resected HCC specimens. IMS revealed the increase of PC phosphatidylcholine (PC) species with palmitoleic acid or oleic acid at the sn-2-position and the reduction of lysophosphatidylcholine (LPC) with palmitic acid at the sn-1-position in HCC tissues.Next, we analyzed mRNA and protein of lysophosphatidylcholine acyltransferase (LPCAT1) which catalyzed LPC into PC and had substrate specificity for some kind of fatty acids.LPCAT1 was more abundant in HCCs than in surrounding parenchyma. We examined the effect of LPCAT1 overexpression or knockdown LPCAT1 on cell proliferation, migration, and invasion in HCC cell lines. LPCAT1 overexpression enriched PCs observed in IMS and promoted cell proliferation, migration, and invasion. LPCAT1 knockdown did vice versa.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.2013
Author(s)
Morita Y, Sakaguchi T, Ikegami K, Goto-Inoue N, Hayasaka T, Hang VT, Tanaka H, Harada T, Shibasaki Y, Suzuki A, Fukumoto K, Inaba K, Murakami M, Setou M, Konno H.
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Journal Title
J Hepatol.
Volume: 29
Issue: 2
Pages: 292-299
DOI
Related Report
Peer Reviewed
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