| Project/Area Number |
22791298
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 食道癌 / 化学療法 / RPA / 感受性試験 / 集学的治療 / 蛋白アレイ / シグナル伝達 / アポトーシス |
|---|
| Research Abstract |
An accurate culture drug sensitivity test may redound to chemotherapy for cancer. In this study, we attempted to establish a new predicting system for drug sensitivity by monitoring molecular signaling pathways in esophageal cancer(ESC) cell line. We firstly evaluated cell signaling affected by CDDP, a key drug for ESC chemotherapy in ESC cell lines. However, it was difficult to interpret the behavior of examined molecules, because multiple pathways were intricately involved in CDDP stimulation. As more simple analysis object, we verified to monitor the detailed cell signaling affected by cetuximab in colorectal cancer cell line HT29 at the protein level using reversephase protain lysate microarrays, with the aim of establishing an accurate analysis, in addition to the conventional Western blotting. In EGF pathway, we were able to quantitative monitor by variation of dephosphorylated protein with time.
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