Molecular biology of GAG in the central nerve system and spinal cord injury.
| Project/Area Number |
22791369
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Aichi Medical University (2011)
Nagoya University (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 脊髄損傷 / ヒアルロン酸オリゴ / 軸索伸長効果 / PKC,リン酸化PKC / 培養上清TNF-α, IL-1β、IL-6活性 / 急性期、亜急性期 / くも膜下、経口投与 / サル脊髄損傷モデル / 培養上清TNF-α,IL-1β、IL-6活性 / NMDA誘導神経細胞死 / PI calcein AM染色 / 培養上清LDH活性 / BDAトレーサー / Iba-1陽性領域 / ED-1陽性領域 |
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| Research Abstract |
Hyaluronan is a component of the extracellular matrix of the central nervous system, and forms perineuronal nets around neurons. It has been recently reported that the hyaluronan-degrading enzyme hyaluronidase promotes lateral mobility of AMPA-type glutamate receptors and enhances synaptic plasticity. However, the biological significance of hyaluronan-degrading products(oligosaccharides) has not been studied in depth. Here we investigated the effects of hyaluronan oligosaccharides on motor function recovery after spinal cord injury in rats. The disaccharide HA2 and especially the tetrasaccharide HA4, significantly improved motor function, unlike the case with oligosaccharides composed of 6-12 saccharides. Consistent with this finding, HA4 treatment enhanced axonal regeneration/sprouting, as assessed by corticospinal tract tracer fiber counts. HA4 treatment also significantly suppressed accumulation of Iba-1-positive cells in a lesion two weeks after injury. In vitro experiments demonstrated that NMDA-induced neuronal cell death was partly blocked by HA4, but not by other oligosaccharides, whereas proteoglycan-mediated inhibition of neurite outgrowth was not affected by treatment with any oligosaccharide examined. Taken together, the present results revealed that due in part to its neuroprotective activity, HA4 promotes motor function recovery after spinal cord injury.
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Report
(3 results)
Research Products
(36 results)
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[Journal Article] An arterial pulse examination is not sufficient for diagnosis of peripheral arterial disease in lumbar spinal canal stenosis : a prospective multicenter study2011
Author(s)
Imagama S, Matsuyama Y, Sakai Y, Ito Z, Wakao N, Deguchi M, Hachiya Y, Osawa Y, Yoshihara H, Kamiya M, Kanemura T, Kato F, Yukawa Y, Yoshida T, Harada A, Kawakami N, Suzuki K, Matsubara Y, Goto M, Sato K, Maruyama K, Yanase M, Ishida Y, Kuno N, Hasegawa T, Ishiguro N
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Journal Title
NAID
Related Report
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[Journal Article] Clinical Outcome of Treatment for Spinal Dural Arteriovenous Fistulas : the Results of Multivariate Analysis and Review of the Literatures2011
Author(s)
Wakao N, Imagama S, Ito Z, Ando K, Hirano K, Tauchi R, Muramoto A, Matsui H, Matsumoto T, Matsuyama Y, Ishiguro N
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Journal Title
NAID
Related Report
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[Journal Article] Differentiation of localization of spinal hemangioblastomas based on imaging and pathological findings2011
Author(s)
Imagama S, Ito Z, Wakao N, Sakai Y, Kato F, Yukawa Y, Sato K, Ando K, Hirano K, Tauchi R, Muramoto A, Hashizume Y, Matsuyama Y, Ishiguro N
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Journal Title
Related Report
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[Journal Article] Total En Bloc Spondylectomy for L2 Chordoma : A case report2011
Author(s)
Wakao N, Imagama S, Ito Z, Ando K, Hirano K, Tauchi R, Muramoto A, Matsui H, Matsumoto T, Matsuyama Y, Ishiguro N
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Journal Title
Nagoya J Med Sci
Volume: (in press)
Related Report
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[Journal Article] Treatment for primary spinal atypical teratoid/rhabdoid tumor2011
Author(s)
Imagama S, Wakao N, Ando K, Hirano K, Tauchi R, Muramoto A, Matsui H, Matsumoto T, Ukai J, Kobayashi K, Shinjo R, Nakashima H, Maruyama K, Matsuyama Y, Ishiguro N
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Journal Title
J Orthop Sci
Volume: (in press)
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