The mechanism of non-steroidal anti-inflammatory drugs on osteoclast differentiation and activation.

• Project/Area Number: 22791800
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Showa University
• Principal Investigator: KARAKAWA Akiko 昭和大学, 歯学部, 助教 (70552280)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯科薬理学 / 破骨細胞 / NSAIDs / COX / 歯学 / osteoclast / cyclooxygenase

Research Abstract

Non-steroidal anti-inflammatory drugs(NSAIDs) have regulatory effect on bone remodeling. The efficacy of NSAIDs on bone remodeling is controversial. In clinics, NSAIDs progress bone repair as their inhibitory effect of inflammatory factors ; in contrast, NSAIDs delay bone fracture healing in animal models. The aim of this study was to elucidate the mechanism of NSAIDs on osteoclast differentiation and bone resorptive activity.
Cyclloxygenase(COX)-1 and-2 transcripts were appeared in early stage of osteoclast differentiation(day3). COX-2 increased on mature osteoclasts and lipopolysaccharide stimulated inflammatory mature osteoclasts. Level of COX-2 transcript has efficacy on extracellular signal-regulated kinase appearance. These results showed that COX-1 and-2 involved in osteoclasts have regulatory effect on osteoclasts differentiation and bone resorptive activity.

Research Products

• [Journal Article] Calcium flux during growth in sham-operated and ovariectomized rats (2011)
  • Author(s): Morohashi T, Karakawa A, Sakai N, Yamada S
  • Journal Title: Dent Med Res Volume: 31 Pages: 1-6
  • NAID: 10027973457
  • Peer Reviewed
• [Journal Article] Effects of tetracycline hydrochloride on measurements with the laser fluorescence device DIAGNOdent : in vitro and in vivo studies (2011)
  • Author(s): Nagaoka E, Morohashi T, Kinoshita J, Karakawa A, Sakai N, Yamada S, Inoue M
  • Journal Title: Lasers Med Sci Volume: 27 Pages: 365-70
  • Peer Reviewed
• [Journal Article] Effects of tetracycline hydrochloride on measurements with the laser fluorescence device DIAGNOdent : in vitro and in vivo studies. (2011)
  • Author(s): Nagaoka E, Morohashi T, Kinoshita J, Karakawa A, Sakai N, Yamada S, Inoue M.
  • Journal Title: Lasers in Medical Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] Calcium flux during growth in sham-operated and ovariectomized rats. (2011)
  • Author(s): Tomio MOROHASHI, Akiko KARAKAWA, Nobuhiro SAKAI, Shoji YAMADA
  • Journal Title: Dental Medicine Research Volume: 31 Pages: 1-6
  • NAID: 10027973457
  • Peer Reviewed
• [Presentation] TLR2アゴニストであるPam3CSK4による破骨細胞形成とラット上顎口蓋骨骨吸収作用について (2011)
  • Author(s): 矢野亜希子, 鈴木恵子, 諸橋富夫, 唐川亜希子, 山本松男, 山田庄司
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-27
• [Presentation] TLR2アゴニストであるPam3CSK4による破骨細胞形成とラット上顎口蓋骨骨吸収作用について (2011)
  • Author(s): 矢野亜希子, 鈴木恵子, 諸橋富夫, 唐川亜希子, 山本松男, 山田庄司
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-07-27
• [Presentation] Pam3CSK4, a TLR2 Agonist, Induces in vitro Osteoclastogenesis RANKL Independently and Resorbs Alveolar Bone in Rats (2011)
  • Author(s): Yano A, Suzuki K, Morohashi T, Arimoto T, Karakawa A, Sakai N, Igarashi T, Yamamoto M, Yamada S
  • Organizer: 第31回昭和歯学会総会
  • Place of Presentation: 昭和大学
  • Year and Date: 2011-07-02
• [Presentation] Pam3CSK4, a TLR2 agonist, induces in vitro osteoclastogenesis RANKL-independently and resorbs alveolar bone in rats (2011)
  • Author(s): Yano A, Suzuki K, Morohashi T, Arimoto T, Karakawa A, Igarashi T, Shinoda H, Yamamoto M, Yamada S
  • Organizer: ASBMR 2011 Annual Meeting
  • Place of Presentation: アメリカ合衆国
• [Presentation] Pam3CSK4, a TLR2 agonist, induces in vitro osteoclastogenesis RANKL-independently and resorbs alveolar bone in rats (2011)
  • Author(s): Akiko Yano, Keiko Suzuki, Tomio Morohashi, Takafumi Arimoto, Akiko Karakawa, Takeshi Igarashi, Hisashi Shinoda, Matsuo Yamamoto, Shoji Yamada
  • Organizer: ASBMR 2011 Annual Meeting
  • Place of Presentation: アメリカ
• [Presentation] Effects of tetracycline hydrochloride on measurements with the laser fluorescence device DIAGNOdent : in vitro and in vivo studies (2010)
  • Author(s): Nagaoka E, Morohashi T, Kinoshita J, Karakawa A, Sakai N, Yamada S, Inoue M.
  • Organizer: 第30回昭和歯学会例会
  • Place of Presentation: 昭和大学
  • Year and Date: 2010-12-04
• [Presentation] Effects of tetracycline hydrochloride on measurements with the laser fluorescence device DIAGNOdent : in vitro and in vivo studies. (2010)
  • Author(s): E.Nagaoka, T.Morohashi, J.Kinoshita, A.Karakawa, N.Sakai, S.Yamada, M.Inoue
  • Organizer: 第30回昭和歯学会例会
  • Place of Presentation: 昭和大学
  • Year and Date: 2010-12-04
• [Presentation] Non-steroidal Anti-inflammatory Drugs Inhibit Osteoclast Activation by Inhibiting Nuclear Translocation of NFκB. (2010)
  • Author(s): A.Karakawa, H.Amano, K.Suzuki, S.Yamada
  • Organizer: ASBMR 2010 Annual Meeting
  • Place of Presentation: カナダ(トロント)
  • Year and Date: 2010-10-17
• [Presentation] Non-steroidal Anti-inflammatory Drugs Inhibit Osteoclast Activation by Inhibiting Nuclear Translocation of NF k appa B (2010)
  • Author(s): Karakawa A, Amano H, Suzuki K, and Yamada S
  • Organizer: ASBMR 2010 Annual Meeting
  • Place of Presentation: カナダ
  • Year and Date: 2010-10-07
• [Remarks] ホームページ昭和大学研究成果データベース
  • URL: https://rdb.showa-u.ac.jp/