Survival of salivary pleomorphic adenoma cells in hypoxic condition
| Project/Area Number |
22791810
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 実験腫瘍学 / 唾液腺多形性線種 / 低酸素 / HIF-1α / VEGF / VHL / SM-AP / perlecan / fibronectin / 唾液腺多形性腺腫 / pVHL / Perlecan |
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| Research Abstract |
Salivary pleomorphic adenoma(PA) is histopathologically characaterized by its colorful stroma with myxoid appearances, which are poorly vascularized, and PA cells are supposed to be able to survive in hypoxic conditions. To understand the hypoxia-dependent manner of cellular proliferation, we determined both protein and gene expression levels of hypoxia-inducible factor 1α(HIF-1α), vascular endothelial growth factor(VEGF), and von Hippel-Lindau(vHL), which degrades HIF-1α. SM-AP cells, which I established from a human parotid pleomorphic adenoma, showed more enhanced levels of HIF-1α, and their VEGF protein levels were kept higher in hypoxic conditions than in aerobic ones. SM-AP cells showed lower expression levels for the vHL gene. Likewise, tumor tissue masses of transplanted SM-AP cells were lower in O_2 concentration than normal subcutanous tissue. The results indicate that pleomorphic adenoma cells can proliferate in the hypoxic condition in which HIF-1αprotein levels were maintained in higher levels because the degradation of HIF-1αwas inhibited due to the lower vHL protein levels. It is thus concluded that hypoxia is more beneficial for PA cell proliferation.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Short telomeres in an oral precancerous lesion : Q-FISH analysis of leukoplakia2012
Author(s)
Aida J, Kobayashi T, Saku T, Yamaguchi M, Shimomura N, Nakamura Kl, Ishikawa N, Maruyama S, Cheng J, Poon SS, Sawabe M, Arai T, Takubo K
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Journal Title
J Oral Pathol Med
Volume: 41(5)
Pages: 372-378
Related Report
Peer Reviewed
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[Journal Article] Short telomeres in an oral precancerous lesion : Q-FISH analysis of leukoplakia2012
Author(s)
Aida J, Kobayashi T, Saku T, Yamaguchi M, Shimomura N, Nakamura KI, Ishikawa N, Maruyama S, Cheng J, Poon SS, Sawabe M, aida T, Takubo K.
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Journal Title
Journal of Oral Pathology & Medicine
Volume: 41(5)
Issue: 5
Pages: 372-378
DOI
Related Report
Peer Reviewed
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[Journal Article] Emergence of keratin 17 vs. loss of keratin 13 : Their reciprocal immunohistochemical profiles in oral carcinoma in situ2011
Author(s)
Mikami T, Cheng J, Maruyama S, Kobayashi T, Funayama A, Yamazaki M, Adeola HA, Wu L, Shingaki S, Saito C, Saku T
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Journal Title
Oral Oncol
Volume: 47(6)
Pages: 497-503
Related Report
Peer Reviewed
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[Journal Article] Emergence of keratin 17 vs. loss of keratin 13 : Their reciprocal immunohistochemical profiles in oral carcinoma in situ2011
Author(s)
Mikami T, Cheng J, Maruyama S, Kobayashi T, Funayama A, Yamazaki M, Adeola HA, Wu L, Shingaki S, Saito C, Saku T.
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Journal Title
Oral Oncology
Volume: 47(6)
Issue: 6
Pages: 497-503
DOI
Related Report
Peer Reviewed
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