Development and Evaluation of Novel Bone Augmentation Strategy through Cellular Engineering Using Epigenetic Regulation

• Project/Area Number: 22791873
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Prosthetic dentistry
• Research Institution: Niigata University
• Principal Investigator: AKIBA Yosuke 新潟大学, 医歯学系, 助教 (70547512)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: エピジェネティクス / 骨造成 / HDACI / 骨再生医療 / 再生医学 / 歯学 / 細胞・組織 / 発生・分化 / 生体材料

Research Abstract

Histone deacetylase inhibitor(HDACI) increases transcriptional activity through chromatin remodeling regulation. In this study, we investigated the possibility and mechanism of bone augmentation mediated by osteogenic cells treated with HDACI and systematic administration effect of HDACI on bone metabolism.
HDACI treated cells showed higher mineralization ability than control. HDACI administration induced faster bone formation and bone defect healing.

Research Products

• [Journal Article] Analysis of patients visiting Niigata Medical and Dental Hospital with chief complaints of dental metal allergy and/or dental focal infection in the previous 8 years (2011)
  • Author(s): Akiba Y., Tomizuka K., Kaku M., Kawasaki M., Nagasawa M., Takano R., Uoshima K
  • Journal Title: The Indonesian Journal of Dental Research Volume: 1(2) Pages: 97-103
  • Peer Reviewed
• [Journal Article] ヒストン脱アセチル化酵素阻害剤(HDACI)を用いたエピジェネティクス制御による新規骨造成法に関する研究 (2011)
  • Author(s): 秋葉陽介・魚島勝美
  • Journal Title: 日本歯科医学会雑誌 Volume: 30 Pages: 83-83
  • Peer Reviewed
• [Presentation] Histone Deacetylase inhibitors have osteogenic differentiation effect on periosteum and periodontal ligament cells (2011)
  • Author(s): Bhuiyan A. A., Akiba Y., Kaku M., Takano R., Uoshima K.
  • Organizer: 平成23年度新潟歯学会第2回例会
  • Place of Presentation: 新潟
  • Year and Date: 2011-11-12
• [Presentation] Histone Deacetylase inhibitors have osteogenic differentiation effect on periosteum and periodontal ligament cells (2011)
  • Author(s): Bhuiyan A.A., Akiba Y.
  • Organizer: 1)平成23年度新潟歯学会第2回例会
  • Place of Presentation: 新潟大学歯学部2階講堂(新潟)
  • Year and Date: 2011-11-12
• [Presentation] Histone Deacetylase inhibitors have osteogenic differentiation effect on periosteum and periodontal ligament cells (2011)
  • Author(s): Bhuiyan A. A., Akiba Y., Kaku M., Takano R., Uoshima K.
  • Organizer: 日本口腔インプラント学会第41回学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-17
• [Presentation] Histone Deacetylase inhibitors have osteogenic differentiation effect on periosteum and periodontal ligament cells (2011)
  • Author(s): Bhuiyan A.A., Akiba Y.
  • Organizer: 第41回日本口腔インプラント学会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-09-17
• [Presentation] Effect of Histone Deacetylase Inhibitor on bone regeneration in Rat (2011)
  • Author(s): Mamunur R. M., Akiba Y., Kaku K., Nagasawa M., Uoshima K.
  • Organizer: 120th Commemorative Scientific Meeting of Japan Prosthodontic Society(International Session)第120回日本補綴歯科学会
  • Place of Presentation: 広島
  • Year and Date: 2011-05-20
• [Presentation] Effect of Histone Deacetylase Inhibitor on bone regeneration in rat (2011)
  • Author(s): Mamunur. R.M., Akiba. Y
  • Organizer: 第120回日本補綴歯科学会
  • Place of Presentation: 広島国際会議場(広島)
  • Year and Date: 2011-05-20
• [Presentation] COL12A1 Gene Silencing Enhances Osteoblastic Differentiation of Human PDL Cells (2011)
  • Author(s): Kaku M., Rosales JM., Akiba Y., Nozawa M., Uoshima K.
  • Organizer: IADR/AADR 89th General Session
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-15
• [Presentation] Development and evaluation of zirconium-dioxide dental implant drill (2011)
  • Author(s): Akiba Y., Mamunur R. M., Nagasawa M., Uoshima K.
  • Organizer: IADR/AADR 89th General Session
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-03-15
• [Presentation] 機械的刺激はコラーゲン分子の翻訳後修飾を介して歯根膜組織の安定化に寄与する (2011)
  • Author(s): 加来賢,秋葉陽介,ロサレス・マルセロ,野沢恩美,魚島勝美
  • Organizer: 口腔先端応用医科学研究会,第3回学術会議
  • Place of Presentation: 東京
  • Year and Date: 2011-01-22
• [Presentation] ヒト口腔内由来幹細胞にヒストン脱アセチル化阻害剤を用いた新規骨造成法に関する研究 (2011)
  • Author(s): 秋葉陽介,加来賢,魚島勝美
  • Organizer: 口腔先端応用医科学研究会,第3回学術会議
  • Place of Presentation: 東京
  • Year and Date: 2011-01-22
• [Presentation] ヒストン脱アセチル化酵素阻害剤(HDACI)を用いたエピジェネティクス制御による新規骨造成法に関する研究 (2011)
  • Author(s): 秋葉陽介,魚島勝美
  • Organizer: 第27回歯科医学を中心とした総合的な研究を推進する集い
  • Place of Presentation: 東京
  • Year and Date: 2011-01-08
• [Presentation] Type XII Collagen Modulates Matrix Formation and Mineralization on Human PDL Cells (2010)
  • Author(s): Kaku M., Rosales JM., Akiba Y., Nozawa M., Uoshima K.
  • Organizer: International Joint Symposium on Oral Science
  • Place of Presentation: Bali, INDONESIA
  • Year and Date: 2010-12-17
• [Presentation] Mineralization Ability of Periosteum Derived Cells (2010)
  • Author(s): Rosales J. M., Kaku M., Akiba Y., Kawasaki M. Nagasawa M., Uoshima K.
  • Organizer: IADR/AADR 88th General Session
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-15
• [Presentation] Effect of HDACIs on mesenchymal stem cell in osteogenic differentiation (2010)
  • Author(s): Akiba Y., Kaku M., Nagasawa M., Bhuiyan A., Uoshima K.
  • Organizer: IADR/AADR 88th General Session
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2010-07-15
• [Presentation] コラーゲン修飾酵素の特異的発現が機械的刺激による歯根膜組織の安定化を制御する (2010)
  • Author(s): 加来賢,川崎真依子,秋葉陽介,ロサレス・マルセロ,ラシッド・マムヌル,魚島勝美
  • Organizer: 第119回日本補綴歯科学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-12
• [Presentation] 間葉系幹細胞の骨誘導におけるヒストン脱アセチル化阻害剤の影響について (2010)
  • Author(s): 秋葉陽介,アルアミン=ブイアン,川崎真依子,加来賢,魚島勝美
  • Organizer: 第119回日本補綴歯科学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-12
• [Presentation] 間葉系幹細胞の骨形成系細胞誘導におけるヒストン脱アセチル化阻害剤の影響について (2010)
  • Author(s): 秋葉陽介・魚島勝美
  • Organizer: 日本補綴歯科学会・学術大会
  • Place of Presentation: 東京ビックサイト