Basic Study on Application of the Amnion and Amniotic Mesenchymal Cells Derived from Cleft of Lip and Palate Patients Themselves.

• Project/Area Number: 22791966
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: University of Toyama
• Principal Investigator: HIROAKI Tsuno 富山大学, 大学病院, 医員 (70377290)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口唇口蓋裂 / 再生医学 / 羊膜

Research Abstract

In this study, msenchymal cells were isolated from the amnion and they were then induced to osteogenic differentiation. The cells demonstrated exellent osteogenic differentiation abitiy that suggested the cell would be applicated to bone tissue engineering. In addition, on the animal study assuming application of dried amniotic membrane to cleft of palate operation, the effect of the membrane on jaw growth following palate operation was indicated. These results suggested that the amion would be a useful material for the treatment of cleft of lip and palate patients.

Research Products

• [Journal Article] Isolation and Characterization of Human Amniotic Mesenchymal Stem Cells and Their Chondrogenic Differentiation (2012)
  • Author(s): Nogami M, Tsuno H, Koike C, et al
  • Journal Title: Transplantation
  • Peer Reviewed
• [Presentation] 口蓋粘膜欠損部へのヒト乾燥羊膜の応用に関する基礎的研究 (2011)
  • Author(s): 津野宏彰
  • Organizer: 第35回日本口蓋裂学会総会・学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2011-05-26
• [Presentation] 口蓋粘膜欠損部へのヒト乾燥羊膜の応用に関する基礎的研究 (2011)
  • Author(s): 津野宏彰
  • Organizer: 第35回日本口蓋裂学会総会・学術大会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2011-05-26
• [Presentation] Application of induced human amniotic mesenchymal cells for bone regenerative therapy (2010)
  • Author(s): Tsuno H
  • Organizer: 9th Asian Congress on Oral and Maxillofacial Surgery
  • Place of Presentation: Kuala Lumpur, Malasia
  • Year and Date: 2010-11-26
• [Presentation] The application of induced human amniotic mesenchymal cells for bone regenerative therapy (2010)
  • Author(s): Hiroaki Tsuno
  • Organizer: 9^<th> Asian Congress on Oral and Maxillofacial Surgery
  • Place of Presentation: Kuala Lunpur Convention Center (Malaysia)
  • Year and Date: 2010-11-26