Global analysis of adipocytes co-cultured with macrophages treated with anti-inflammatory substance
| Project/Area Number |
22792086
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontal dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMASHITA Akiko 広島大学, 大学院・医歯薬学総合研究科, 助教 (70511319)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | アディポサイトカイン / 歯周病 / メタボリックシンドローム / 抗炎症作用 / 脂肪細胞 / マクロファージ / フラボノイド |
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| Research Abstract |
We analyzed protein and genes differentially expressed in LPS stimulated adipocytes when co-cultured with macrophages in the presence of epicatechin. Murine macrophage cell line and murine adipocytes were co-cultured. We used DNA microarray method, Real-time PCR method and so on. According to the results, we revealed that epicatechin reduced LPS-induced inflammatory reaction, it depressed NF-kB transcriptional regulation, and LPS-induced oxidative stress reaction was eased by epicatecin.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Adipocyte-macrophage interaction may mediate LPS-induced low-grade inflammation : potentiallink with metabolic complicationsInnate Immunity2012
Author(s)
Nakarai H., Yamashita A., Nagayasu S., Iwashita M., Kumamoto S., Ohyama H., Hata M., Soga Y., Kushiyama A., Asano T., Abiko Y., Nishimura F
Volume
18(1)
Pages
164-170
Related Report
Peer Reviewed
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