Investigation of redox regulation by Keap-1 and Nrf2 system in periodontal tissue

• Project/Area Number: 22792089
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: BANDO Mika 徳島大学, 病院, 医員 (10510000)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯周組織由来細胞 / 酸化ストレス / Keap1-Nrf2 / 歯肉上皮細胞

Research Abstract

The aim of this study is investigation of redox regulation in periodontal tissue to use antioxidative stress mechanism(Keap1-Nrf2 system) as new periodontitis prevention and treatment. This study show Porphyromonas gingivalis(p. g.) LPS induced oxidative stress and the antioxidant response in human gingival fibroblast. keratinocyte growth factor(KGF) induced antioxidative enzyme(HO-1) and antioxidant response-transcriptional factor(Nrf2) expressions. KGF may enhance antioxidative stress mechanism in periodontal tissue.

Research Products

• [Journal Article] Analysis of proteins in human gingival crevicular fluid by mass spectrometry (2012)
  • Author(s): Kido J, Bando M, Hiroshima Y et al.
  • Journal Title: J Periodont Res Volume: (in press) Issue: 4 Pages: 488-499
  • DOI: 10.1111/j.1600-0765.2011.01458.x
  • Peer Reviewed
• [Journal Article] Resistin in gingival crevicular fluid and induction of resistin release by Porphyromonas gingivalis lipopoly saccharide in human neutrophil (2012)
  • Author(s): Hiroshima Y, Bando M, Inagaki Y et al.
  • Journal Title: J Periodont Res Volume: (in press) Issue: 5 Pages: 554-652
  • DOI: 10.1111/j.1600-0765.2011.01466.x
  • Peer Reviewed
• [Journal Article] Regulation of antimicrobial peptide expression in human gingival keratinocytes by interleukin-1α (2011)
  • Author(s): Hiroshima Y, Mika Bando et al.
  • Journal Title: Arch Oral Biol Volume: 56 Pages: 761-767
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21316034
• [Journal Article] Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1α (2010)
  • Author(s): Mika Bando, Hiroshima Y et al.
  • Journal Title: Immunology and Cell Biology Volume: 88 Pages: 328-333
  • URL: http://www.nature.com/icb/journal/v88/n3/full/icb2009104a.html
  • Peer Reviewed
• [Presentation] 最終糖化産物はマクロファージ様細胞の炎症関連因子の発現を調節する (2011)
  • Author(s): 村田裕美, 木戸淳一, 板東美香, 他
  • Organizer: 第135回秋季日本歯科保存学会
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2011-10-21
• [Presentation] 歯肉溝滲出液中のYKL-40の分析 (2011)
  • Author(s): 坂東由記子, 木戸淳一, 廣島佑香, 板東美香, 他
  • Organizer: 第54回日本歯周病学会秋季学術大会
  • Place of Presentation: 海峡メッセ下関(山口県)
  • Year and Date: 2011-09-24
• [Presentation] 歯肉溝滲出液中のグリコアルブミン測定法の確立 (2011)
  • Author(s): 板東美香, 木戸淳一、稲垣裕司, 他
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-05-27