Structure and function of a novel signal transducer protein that employs heme-PAS domain as a sensor

• Project/Area Number: 22870043
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2011); National Institutes of Natural Sciences Okazaki Research Facilities (2010)
• Principal Investigator: SAWAI Hitomi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特任助教 (50584851)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: ヘムタンパク質 / センサータンパク質 / シグナル伝達タンパク質 / 走化性制御 / ヘム含有PASドメイン / 共鳴ラマン分光法 / X線結晶構造解析 / 酸素センサー / PASドメイン

Research Abstract

I studied the structure and function of Aer2, a novel signal transducer protein for bacterial aerotaxis control. Spectroscopy and site-directed mutagenesis revealed that Aer2 was a new type of oxygen sensor protein employing a heme as a sensor. To elucidate the molecular mechanisms of oxygen sensing and signal transduction, I characterized Aer2 by X-ray crystallography and resonance Raman spectroscopy.

Research Products

• [Journal Article] Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginose (2012)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Yoshitsugu Shiro, Haruto Ishikawa, Yasuhisa Mizutani, Shigetoshi Aono
  • Journal Title: Chemical Communications Volume: 48 Pages: 6523-6525
  • Peer Reviewed
• [Journal Article] Crystal Structure of Carbonmonoxide Complex of Human Cytoglobin (2011)
  • Author(s): M.Makino, H.Sawai, Y.Shiro, H.Sugimoto
  • Journal Title: PROTEINS Volume: 79 Issue: 4 Pages: 1143-1153
  • DOI: 10.1002/prot.22950
  • Peer Reviewed
• [Journal Article] Aldoxime Dehydratase : Probing the Heme Environment Involved in the Synthesis of the Carbon-Nitrogen Triple Bond (2011)
  • Author(s): Eftychia Pinakoulaki, Constantinos Koutsoupakis, Hitomi Sawai, Andrea Pavlou, Yasuo Kato, Yasuhisa Asano, Shigetoshi Aono
  • Journal Title: The Journal of Physical Chemistry B Volume: 115 Pages: 13012-13018
  • Peer Reviewed
• [Presentation] Structural characterization of Aer2 that employs a heme as an oxygen sensor for aerotaxis control (2012)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yasuhisa Mizutani, Yoshitsugu Shiro, Shigetoshi Aono
  • Organizer: 4^<th> Japan-Korea Seminars on Biomolecular Sciences : Experiments and Simulations
  • Place of Presentation: Todaiji Cultural Center(Japan)
• [Presentation] Structural characterization of Aer2 that employs a heme as an oxygen sensor for aerotaxis control (2012)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yasuhisa Mizutani, Yoshitsugu Shiro, Shigetoshi Aono
  • Organizer: 4^<th> Japan-Korea Seminars on Biomolecular Sciences : Experiments and Simulations
  • Place of Presentation: 東大寺総合文化センター(奈良県)
• [Presentation] 新規な転写調節因子HesRのヘムによる機能制御の分子機構 (2012)
  • Author(s): 澤井仁美、杉本宏、山中優、城宜嗣、青野重利
  • Organizer: 第92回日本化学会春季年会
  • Place of Presentation: 慶応大学(神奈川県)
• [Presentation] Structure and function of oxygen sensor proteins adopting a heme-Containing PAS domain as a sensor for aerotaxis control (2011)
  • Author(s): Shigetoshi Aono
  • Organizer: Third Korea-Japan Seminars on Biomolecular Sciences Experiments and Simulations
  • Place of Presentation: Lotte Hotel, Jeju (Korea)
  • Year and Date: 2011-03-01
• [Presentation] Structural characterization of Aer2 that adopts a heme-containing PAS domain as an oxygen sensor for aerotaxis control (2011)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yoshitsugu Shiro, Yasuhisa Mizutani, Shigetoshi Aono
  • Organizer: 15^<th> International Conference on Biological Inorganic Chemistry(ICBIC15)
  • Place of Presentation: University of British Colombia(Canada)
• [Presentation] ヘム分子により機能制御される新規な転写調節因子HesRのX線結晶構造 (2011)
  • Author(s): 澤井仁美、山中優、杉本宏、城宜嗣、青野重利
  • Organizer: 第5回バイオ関連化学合同シンポジウム
  • Place of Presentation: つくば国際会議場(茨城県)
• [Presentation] Structural characterization of Aer2 that adopts a heme-containing PAS domain as an oxygen sensor for aerotaxis control (2011)
  • Author(s): Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yoshitsugu Shiro, Yasuhisa Mizutani, Shigetoshi Aono
  • Organizer: 15^<th> International Conference on Biological Inorganic Chemistry (ICBIC15)
  • Place of Presentation: University of British Colombia, Vancouver (Canada)
• [Presentation] ヘムにより機能制御される新規な転写調節因子HesRのX線結晶構造 (2011)
  • Author(s): 澤井仁美、杉本宏、山中優、城宜嗣、青野重利
  • Organizer: 平成23年度日本結晶学会年会
  • Place of Presentation: 北海道大学(北海道)
• [Presentation] 新規なヘム含有O_2センサー蛋白質Aer2における気体分子の識別感知機構 (2011)
  • Author(s): 澤井仁美
  • Organizer: 岡崎統合バイオサイエンスセンター10周年記念シンポジウム
  • Place of Presentation: 岡崎統合バイオサイエンスセンター(愛知県)
• [Presentation] New oxygen sensor protein adopting a heme-containing PAS domain asa sensor for aerotaxis control (2010)
  • Author(s): Shigetoshi Aono
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2010)
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii(USA)
  • Year and Date: 2010-12-16
• [Presentation] ヘム含有PASドメインを有する新規な走化性制御蛋白質Aer2の酸素感知機構 (2010)
  • Author(s): 澤井仁美
  • Organizer: 第4回バイオ関連化学シンポジウム
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2010-09-25
• [Presentation] ヘム含有PASドメインを有する新規な走化性制御蛋白質Aer2の酸素感知機構 (2010)
  • Author(s): 澤井仁美、石川春人、水谷泰久、青野重利
  • Organizer: 第4回バイオ関連化学合同シンポジウム
  • Place of Presentation: 大阪大学(大阪府)
• [Presentation] ガス分子により駆動される新規なセンサータンパク質の機能発現機構 (2010)
  • Author(s): 澤井仁美、青野重利
  • Organizer: 平成22年度特定領域「細胞感覚」夏の班会議
  • Place of Presentation: 札幌市教育文化会館(北海道)
• [Presentation] ガス分子により駆動される新規なセンサータンパク質の機能発現機構 (2010)
  • Author(s): 澤井仁美
  • Organizer: 平成22年度特定領域研究「細胞感覚」夏の班会議
  • Place of Presentation: 札幌市教育文化会館(北海道)