Role of Exol in germ cell apoptosis during spermatogenesis

• Project/Area Number: 22880002
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: NAMIKI Yuka 北海道大学, 大学院・獣医学研究科, 特任助教 (20590417)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 減数分裂 / アポトーシス / Exo1

Research Abstract

MRL/MpJ mouse testes have metaphase-specific apoptosis(msa) in meiotic spermatocytes. In our previous study, msa occurs due to mutation of the exonuclease 1(Exo1) located on Chr1 of 81. 9cM. In this study, in order to find practical factors in msa,(1) we created B6 background congenic mice(Cg1) carrying msa loci derived from MRL mice.(2) I made purified RNA sample for microarray to know mRNA expression level of apoptosis related genes in testes from Cg1 and B6 mice as a control(6 weeks of age).