Project/Area Number |
22880034
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Applied microbiology
|
Research Institution | Jikei University School of Medicine |
Principal Investigator |
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | バイオフィルム / MRSA / セリンプロテアーゼ / プロテオーム解析 / 分子シャペロン / 細胞骨格タンパク質 / 細胞外マトリックス / 細菌間干渉 / 黄色ブドウ球菌 / 細胞外マトリクス / 分泌タンパク質 / 細胞壁アンカータンパク質 / アミロイド線維 / トランスポゾン / 細胞外プロテアーゼ |
Research Abstract |
Staphylococcus aureus exhibits a strong capacity to attach to the surface of implanted medical devices and forms multilayered communities of bacteria, called biofilm. S. aureus is a frequent cause of biofilm-associated infections that are a tremendous burden on our healthcare system. Here, we identified and characterized biofilm-matrix proteins which hold cells and contribute to bacterial accumulation in multiple layers. In addition, we found that extracellular serine protease Esp, secreted by a subset of commensal Staphylococcus epidermidis, inhibits nasal colonization of S. aureus via degradation of S. aureus surface proteins and host receptors that are crucial for biofilm formation and host-pathogen interactions. These findings not only provide novel insights into fundamental principles underlying S. aureus biofilm development, but may also indicate a novel direction for therapeutic intervention against biofilm infections.
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