Degradation of biofilm by fructanase chimeric enzyme

• Project/Area Number: 22890210
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: KAKUTA Erika 鶴見大学, 歯学部, 学部助手 (30585469)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,977,000 (Direct Cost: ¥2,290,000、Indirect Cost: ¥687,000); Fiscal Year 2011: ¥1,417,000 (Direct Cost: ¥1,090,000、Indirect Cost: ¥327,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯学 / 酵素 / 細菌 / 遺伝子 / バイオテクノロジー

Research Abstract

The purposes of this study were to construct chimeric enzyme of dextranase and fructanase, and to degrade the biofilm effectively. The dextranase gene and fructanase gene from S. mutans UA159 were cloned and connected in same plasmid vector. These enzymes were expressed in Escherichia coli. The function of enzyme was investigated by Somogyi-Nelson method using dextran and fructan as substrates. The enzyme degraded dextran and fructan. These results suggested that these enzymes are useful tool for the degradation of biofilm.

Research Products

• [Journal Article] Oral conditions that affect salivary biomarkers-Preliminary survey by self-administrated questionnaire- (2012)
  • Author(s): Erika Kakuta, Akira Ida, Hiromi Nomura, et al.
  • Journal Title: 日本口腔衛生学会雑誌 Volume: 62巻(印刷中)
  • NAID: 110009489302
• [Journal Article] Oral conditions that affect salivary biomarkers-Preliminary survey by self-administrated questionnaire- (2012)
  • Author(s): Erika Kakuta, Akira Ida, Hiromi Nomura, et al
  • Journal Title: 日本口腔衛生学会雑誌 Volume: 62巻(印刷中)
  • NAID: 110009489302
  • Peer Reviewed
• [Presentation] アロマテラピーの歯科応用への模索 (2011)
  • Author(s): 角田衣理加、大島朋子、前田伸子他
  • Organizer: 第14回日本アロマセラピー学会・学術総会
  • Place of Presentation: 日本科学未来館(東京都)
  • Year and Date: 2011-11-12
• [Presentation] アロマテラピーの歯科応用への模索 (2011)
  • Author(s): 角田衣理加、大島朋子、前田伸子, 他
  • Organizer: 第14回日本アロマセラピー学会・学術総会
  • Place of Presentation: 日本科学未来館(東京都)(教育講演)
  • Year and Date: 2011-11-12
• [Presentation] 歯周病スクリーニングにおける唾液検査と問診項目の関連性に関する予備的検討 (2011)
  • Author(s): 角田衣理加、野村義明、大島朋子, 他
  • Organizer: 第60回日本口腔衛生学会・総会
  • Place of Presentation: 日本大学松戸歯学部(千葉県)
  • Year and Date: 2011-10-09
• [Presentation] ムタナーゼとデキストラナーゼ両遺伝子の連結によるバイオフィルムの分解 (2011)
  • Author(s): 角田衣理加、野村義明、村田貴俊, 他
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Book] 歯科発ヘルシーライフプロモーション1章10-2:Sun protection/UV careを誤解していませんか?(監修花田信弘編著武内博朗) (2011)
  • Author(s): 武内博朗、小林和子、角田衣理加
  • Total Pages: 155
  • Publisher: デンタルダイヤモンド社
• [Book] 歯科発ヘルシーライフプロモーション監修1章10-2 : Sun protection/UV careを誤解していませんか
  • Author(s): 武内博朗、小林和子、角田衣理加
  • Total Pages: 155
  • Publisher: デンタルダイヤモンド社