Invitro genome editing in aquaculture

• Project/Area Number: 22K05832
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40040:Aquatic life science-related
• Research Institution: Kyushu University
• Principal Investigator: Chakraborty Tapas 九州大学, 農学研究院, 助教 (70715440)
• Project Period (FY): 2022-04-01 – 2025-03-31
• Project Status: Granted (Fiscal Year 2022)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2024: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Stem cell / genome editing / reproduction / mackerel / surrogacy

Outline of Research at the Start

In this study, using a novel fish stem cell culture methodology, we will culture chub mackerel gonadal stem cell and perform various single-cell analysis (RNA, protein, epigenetics) to elucidate the control mechanism of germline stem cell proliferation and differentiation. In addition, the genome editing experimental system established in this study will be an innovative technology for "in vitro breeding" of farmed fish in the future.