| Project/Area Number |
22K05832
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40040:Aquatic life science-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2024: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | stem cell / fish / culture / pluripotent / chub mackerel / differentiaiton / in vitro / FSH / Stem cell / genome editing / reproduction / mackerel / surrogacy |
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| Outline of Research at the Start |
In this study, using a novel fish stem cell culture methodology, we will culture chub mackerel gonadal stem cell and perform various single-cell analysis (RNA, protein, epigenetics) to elucidate the control mechanism of germline stem cell proliferation and differentiation. In addition, the genome editing experimental system established in this study will be an innovative technology for "in vitro breeding" of farmed fish in the future.
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| Outline of Final Research Achievements |
In vitro breeding, i.e. production of fish egg and sperm in vitro has ann enormous potential for aquaculture in future. Therefore, in this research, I have established the protocol for culture and differentiation, genome editing, clone selection and transplantation of chub mackerel germ line stem cell (GSC). I have created host for transplantation, performed transplantation and produce next generation from genome edited GSC and established that cultured GSC can produce progeny with the aid of appropriate surrogate. I have also differentiated the GSC into germ cell invitro and identified several differentiation factors, including FSH. Though Germ cell differentiation from GSC was achieved but complete differentiation of GSC into functional gamete needs future research. Additionally importance of FSH in fish GSC maintenance or differentiation requires indepth analysis.
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| Academic Significance and Societal Importance of the Research Achievements |
We have establish the culture, in vitro genome editing techniques for chub mackerel GSC and produced genome edited porgeny. These, expectedly will improve and fast track the breeding of new fish strains, ease the species conservation and contribute towards future food and biodiversity security.
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