| Project/Area Number |
22K06028
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
河村 麻紀 山口大学, 総合技術部, 技術専門職員 (40816732)
木村 透 山口大学, 共同獣医学部, 教授 (80419027)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2024: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2023: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 好酸球増多症 / 突然変異マウス / Th2免疫活性 / 好酸球増多 / Th2免疫活性化機構 / 好酸球 / Th2免疫反応 / 免疫 |
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| Outline of Research at the Start |
新規に確立した好酸球増多症を形質として示す突然変異マウスの原因遺伝子の特定とその変異様式の解明を目的として、遺伝子マッピング法によるQTL解析を行い原因遺伝子の特定を行う。特定後に対象遺伝子のシークエンスと発現量の検討を行い変異形式を特定する。
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| Outline of Final Research Achievements |
The chromosome of the gene that was congenic this time was identified as being located on chromosome 8, but the causative gene could not be identified. Even in the case of homozygous individuals obtained by crossbreeding the mice used in the analysis, only individuals with significantly lower eosinophil counts were obtained compared to the high eosinophil counts observed in the Yama mice. This suggests that multiple genes are involved in the increase in eosinophils in the Yama mice, and that the gene that was congenic this time plays a supporting role.
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| Academic Significance and Societal Importance of the Research Achievements |
今回の研究より、Yamaマウスの好酸球増多は複数の遺伝子が関与していることが示唆された。今後は好酸球増多に関する重要な遺伝子の特定を行う事で、好酸球産生機構の解明や引いては、Th2免疫活性化に関する不明であった経路の発見の一助となるとも考えられる。
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