| Project/Area Number |
22K15249
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | Quantum dots / Cell imaging / Transcription Factor / Medaka fish / reactive oxygen species / Molecular modeling / NANO-TF / Silica nanoparticles / Mitochondrial factors / Cell Imaging / medaka egg imaging / Carbon quantum dots / Silicon quantum dots / Transcription factor |
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| Outline of Research at the Start |
Cancer is the leading cause of death worldwide. Recent studies have revealed the importance of reactive oxygen species produced by mitochondrial complex I in cancer disease and their associated signaling pathways and epigenetic regulation. It is believed that this NANO-TF can control ROS levels and intracellular energy
metabolism in patients with cancer disease and provide innovative strategies for treating cancer.
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| Outline of Final Research Achievements |
We have developed water-soluble SiQDs and CQDs for bio-imaging, as well as to prepare the artificial transcription factor. Instead, we used silicon QDs with decene molecules for surface functionalization and Pluronic F127 for the water-soluble process. Additionally, we synthesized CQDs from biomass waste.We tested the cytotoxicity of both materials on various cell lines, including HeLa, A549, fibroblast, iPS, cardiomyocytes, and Japanese medaka fish. We used these materials to develop NANO-TF. The NANO-TF units contain drugs and mitochondrial penetrating peptides, which enable them to penetrate inside the mitochondria.
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| Academic Significance and Societal Importance of the Research Achievements |
当研究室において合成された量子ドットベースの NANO-TF は、ミトコンドリア膜を透過してがん細胞のミトコンドリア DNA に到達する能力を持っており、得られた結果は、QD ベースの薬物送達による癌細胞の破壊に大きく役立つ。
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