| Project/Area Number |
22K15424
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49020:Human pathology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2022-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2022: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | IMP3 / 血管内大細胞型B細胞リンパ腫 / 多重免疫染色 / 二重免疫染色 |
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| Outline of Research at the Start |
血管内大細胞型B細胞リンパ腫（intravascular large B-cell lymphoma; IVL）は生検組織中の腫瘍細胞量が限られ、特異的な診断マーカーにも乏しい事から、しばしば診断が難しい高悪性度リンパ腫である。本研究は、IVLの病理診断において、癌胎児蛋白の一種であるinsulin-like growth factor II mRNA-binding protein 3(IMP3)の有用性を検証することを目的とし、多施設から集積した多くのIVL症例と非IVL症例の試料を用いた後ろ向き研究にて免疫組織化学的手法を用いたIMP動態の解析を行う。
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| Outline of Final Research Achievements |
We analyzed the utility of IMP3 immunohistochemistry for intravascular large B-cell lmyphoma (IVL) diagnosis in various organs. Overall, 152 pathological specimens(111 positive and 41 negative for IVL) obtained from 88 patients with a diagnosis of IVL were stained for IMP3 and IMP3/PAX5. As negative controls, 40 pathology specimens from 38 patients with no history of IVL or other B-cell lymphomas were stained for IMP3. All mononuclear cells with cytoplasmic staining were considered positive for IMP3 expression, but expression restricted to germinal center B cells was excluded from evaluation. All 111 IVL pathological specimens were positive for IMP3 and IMP3/PAX5. In addition,11 of the 41 specimens originally diagnosed as IVL-negative showed IMP3/PAX5 double-positive cells.However,of the 40 negative control samples,IMP3-positive nongerminal center B cells were detected in only 2 samples (P = 0.0131). IMP3 can be a helpful adjunct for IVLBCL diagnosis.
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| Academic Significance and Societal Importance of the Research Achievements |
血管内大細胞型B細胞リンパ腫(intravascular large B-cell lymphoma; IVL)は生検組織
中の腫瘍細胞量が限られ、特異的な診断マーカーにも乏しい事から、しばしば診断が難しい高悪性度リンパ腫である。申請者は癌胎児蛋白の一種であるinsulin-like growth factorII mRNA-binding protein 3 (IMP3)がIVLの腫瘍細胞に高頻度に発現している事を見出し、多施設から集積した多くのIVL症例を用いた後ろ向き研究にて免疫組織化学的手法を用いてIMPの発現を解析し、IVLの病理診断におけるIMP3の有用性を証明した。
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