| Project/Area Number |
22K15656
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 51030:Pathophysiologic neuroscience-related
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
段 ショウキ 兵庫医科大学, 医学部, 助教 (20910607)
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| Project Period (FY) |
2022-04-01 – 2025-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2024: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | SNS / IBS / Micro-inflammation / Visceral pain / sympathetic nervous / eosinophil |
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| Outline of Research at the Start |
Patients who suffer from irritable bowel syndrome often exhibit abnormal psychological profiles like depression, which is closely related to the sympathetic nervous system (SNS) activity. SNS-immune interaction is considered important to chronic disease development. Recently, we successfully established a maternal separation-induced IBS animal model, which showed increased activity of SNS and micro-inflammation in the gastrointestinal (GI) tract. The proposed project aimed to investigate whether and how SNS modulates eosinophil in the GI tract, and their involvement in pathophysiology of IBS.
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| Outline of Annual Research Achievements |
This year, I primarily conducted genetic modulation experiments. Using DBH-cre mice and AAV-PHP.s-Gq, I successfully activated sympathetic nervous system (SNS) activity through CNO injection, as confirmed by double immunohistochemical staining of cre and mcherry, and the NE concentration in the serum. The results indicated that immune cells were recruited into the GI tract following SNS activation, consistent with findings in the MS model. As for the underlying mechanisms, I checked several cytokines and chemokines, and aims to clarify the connection between SNS activity and immune alteration.
I attended two academic conferences this year. I did symposium speaking in the 46th Annual Meeting of the Japan Neuroscience Society. And I did an oral presentation in the 45th Annual Meeting of Japanese Association for the Study of Pain.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I proved that activate SNS recruited immune cells into the GI tract using genetic modulation method. By those data, I achieved the second step of the project.
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| Strategy for Future Research Activity |
In the next year, I will clarify the underlying mechanism that SNS modulates the immune cell immigration. Especially the cytokines, chemokines and related cell type. In addition, I will summarize the data and prepare the manuscript to publish the article.
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