| Project/Area Number |
22K19211
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
太田 耕平 九州大学, 農学研究院, 教授 (10585764)
Chakraborty Tapas 九州大学, 農学研究院, 助教 (70715440)
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| Project Period (FY) |
2022-06-30 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2023: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2022: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | Biomaterial / RISV vaccine / Knock in / Anchovy / Chub mackerel / immunity / biomaterial / Aquaculture / value added fish |
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| Outline of Research at the Start |
世界の人口増加に伴い、高品質な食品に加え、医薬品、サプリメント、ワクチンなど需要も増大し、これらを持続的に供給するシステムが必要になると予測される。しかしながら、生物工場による物質生産は一般的にコストが高く、産業化されているものは限られている。本研究では、多産でライフサイクルの短いカタクチイワシを利用し、ゲノム編集によるノックイン法により、魚卵に有用物質を蓄積させ、これを餌とすることで養殖魚に機能性や高付加価値を付与する。本研究期間ではマダイイリドウイルスに対する「魚卵ワクチン」を生産して免疫付与の 効果を検証する。
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| Outline of Final Research Achievements |
with the emerging issues of food security, abundant and easy production of biomaterial might be a innovative solution for nutritional supplimentation. Therefore to increase the fish vaacciation efficiency and test the fish based biomaterial production possibility, we have produced red sea bream irrido virus (RSIV) capsid protein gene-knock-in anchovy and medaka, capable of producing recombinant capsid protein which is maternally deposited into the progeny egg yolk. We did not observe any abnormal phenotype in any stages of life. when chub mackerels were fed with knock-in embryos they showed enhanced immunity, cellular health and better survibility after infected with RSIV. However, the mode of action and effective duration of infection resistance need to be carefully validated in future. In future, we would like to explore the possibility of more efficient and risk-free production of biomaterial from fish.
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| Academic Significance and Societal Importance of the Research Achievements |
Our data shows that biomaterial production, atleast for easy fish vaccination is possible. in future this will open avenues for new, efficient, inexpensive fish vaccine production for aquaculture sustaiability and revolutionize the food grade pharmaceuticals, supplements production industry.
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