| Project/Area Number |
22K20590
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
0603:Forestry and forest products science, applied aquatic science, and related fields
|
|---|
| Research Institution | Akita University |
|---|
Principal Investigator |
Lam PuiYing 秋田大学, 理工学研究科, 助教 (60961711)
|
|---|
| Project Period (FY) |
2022-08-31 – 2025-03-31
|
| Project Status |
Granted (Fiscal Year 2023)
|
| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | Lignin / Flavonoids / Phenylprppanoids / Metabolic formation / grasses / lignin / flavonoids / phenylpropanoids / metabolon formation |
|---|
| Outline of Research at the Start |
Understanding metabolic flux of lignin and and related flavonoid and phenylpropanoid pathways is essential for designing bioengineering strategies to precisely manipulate lignin and flavonoid composition in grasses to improve their biomass utility for biorefineries. This study is aimed to determine the global organization of metabolons (protein-protein interaction networks) and metabolic channelling of the biosynthetic enzymes of lignin together with the closely-related parallel flavonoid and upstream phenylpropanoid pathway enzymes in grasses.
|
| Outline of Annual Research Achievements |
The proposed research aims to determine the global organization of metabolons (protein-protein interaction networks) and metabolic channelling of the biosynthetic enzymes of lignin together with the closely related parallel flavonoid and upstream phenylpropanoid pathway enzymes in grasses, using rice as a model. Thought in silico analysis, all the putative and known enzymes in the lignin, flavonoid ad phenylpropanoid biosynthetic pathways of rice have been identified. The proposed experiments are currently ongoing.
|
| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
The proposed research is delayed because of the delay of the approval of experiments at Akita University, the delayed arrival of the plant growth chamber due to the global shortage of semi-conductor, and health problems of the applicant. After the approval of the experiments in March, 2023, experiments are currently carrying out as planned.
|
| Strategy for Future Research Activity |
The proposed research will be carried out as described in the proposal with slight modifications as described in the report last year. Cloning are currently ongoing. After finishing cloning, subcellular localization, yeast-two hybrid analysis, and split GAL4 RUBY (a series of betanidin biosynthetic genes) assay of the enzymes in the phenylpropanoid, lignin, and flavonoid biosynthetic pathways of rice will be carried out. Analysis of metabolite channelling will be conducted after the organization of metabolon is determined.
|
