| Project/Area Number |
22K20705
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0801:Pharmaceutical sciences and related fields
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| Research Institution | Hokkaido University |
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Principal Investigator |
タン ロジャー・サルバシオン 北海道大学, 先端生命科学研究院, 助教 (10951759)
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| Project Period (FY) |
2022-08-31 – 2024-03-31
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| Project Status |
Discontinued (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Exosome / Nanosome / Drug Delivery System / Nanoparticles / Exosome-nanosme / Cancer cells / Nanoparticle |
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| Outline of Research at the Start |
Part 1: Exosome target organ determination.
Part 2: Nanosome construction and Drug loading.
Part 3: Efficiency testing of the novel platform.
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| Outline of Annual Research Achievements |
In the extracellular vesicles population produced by cells, exosomes only constitute a small portion of the population. This has been one of the limitations of studying exosomes. One of the optimization protocols necessary and employed in this research was to find a way to induce the production of a higher number of exosomes in cancer cells. After employing different strategies, we were able to successfully induce cancer cells to produce a higher number of exosomes by starving them. Production and isolation of exosomes from starved cancer cells were then optimized to yield a stably high number of exosomes. Starved cancer cells produced a higher number of exosomes (1.56e+15 particles per mL with an average particle size of 139.5 nm) compared to the fed cancer cells (1.51e+10 particles per mL with an average particle size of 106.5 nm). This finding enabled us to optimize the exosome production and yielded considerably more exosomes needed for the study.
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