| Project/Area Number |
22KF0005
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| Project/Area Number (Other) |
21F21390 (2021-2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2021-2022) |
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| Section | 外国 |
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| Review Section |
Basic Section 42030:Animal life science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
中尾 亮 北海道大学, 獣医学研究院, 准教授 (50633955)
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| Co-Investigator(Kenkyū-buntansha) |
MOHAMMED ABDELBASET 北海道大学, 獣医学研究院, 外国人特別研究員
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2023: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2022: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2021: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Coxiella / Endosymbionts / Haemaphysalis / RNAseq / Symbionts / Ticks / Vaccine / コクシエラ様共生菌 / マイクロビオーム / ビタミンB / マダニ / ワクチン |
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| Outline of Research at the Start |
マダニは様々な病原体を保有し、吸血の際に人や動物にそれらを伝播する。一方で、マダニは病原体以外の多様な微生物群を体内に保有し、その一部はマダニの生存に重要な共生菌として機能することが知られつつある。本研究では、マダニの共生菌由来分子のワクチンターゲットとしての可能性を遺伝子発現情報から探究する。研究成果は、マダニ媒介性感染症の予防法の開発やマダニの生存戦略の解明につながる。
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| Outline of Annual Research Achievements |
Coxiella-like endosymbionts (CLEs) are obligate mutualists that was suggested to supplement B vitamins and other metabolites. Molecular mechanisms between CLEs and ticks remain poorly understood. Thus, we performed a transcriptomic analysis of CLE genes expressed in the ovaries and Malpighian tubules of Haemaphysalis longicornis ticks after feeding compared to unfed ones. We constructed the genome of H. longicornis, including symbiotic bacteria, and assembled CLE genes from both organs, based on RNA-seq results. Our data revealed upregulation of genes involved in vitamin B biosynthesis and Gene Ontology terms associated with biotin and ubiquinone biosynthesis. These findings provide a fundamental basis for identifying potential targets in the development of anti-tick control strategies.
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