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Ultra-Precision Agriculture Using Fluorescence Based Label Free Technology for Green Fruit

Research Project

Project/Area Number 22KF0179
Project/Area Number (Other) 21F21397 (2021-2022)
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeMulti-year Fund (2023)
Single-year Grants (2021-2022)
Section外国
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionKyoto University

Principal Investigator

近藤 直 (2021, 2023)  京都大学, 農学研究科, 教授 (20183353)

Co-Investigator(Kenkyū-buntansha) HUANG ZICHEN  京都大学, 農学研究科, 外国人特別研究員
Host Researcher 近藤 直 (2022)  京都大学, 農学研究科, 教授 (20183353)
Foreign Research Fellow HUANG ZICHEN  京都大学, (連合)農学研究科(研究院), 外国人特別研究員
Project Period (FY) 2023-03-08 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2023: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2022: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2021: ¥500,000 (Direct Cost: ¥500,000)
Keywordsimage / fluorescence / grading / green fruit / machine vision / grader / green fruits / fluorescence imaging / label-free / disease
Outline of Research at the Start

There are two topics in this research. The first topic is to build an acoustic-based hybrid localization system for a greenhouse robot. Experiments will be conducted to evaluate its localization performance, including accuracy and Doppler shift to moving objects. The second topic is the application of fluorescence imaging-based sensing methods to the precision agriculture of green peppers. Early-stage diseases will be studied and detected using fluorescence images. Also, a label-free method will be established to track individual peppers.

Outline of Annual Research Achievements

We developed a fluorescence image acquisition system for analyzing the phenotype of green peppers. The fruits had some fluorescence substance whose excitation wavelength was 365 nm and emitted blue fluorescence, which can be captured by cameras within the visible light spectrum. Notably, the "Manganji sweet peppers" in Kyoto, Japan, represent a distinctive variety with significant demand for agricultural big data under their regional brand. In response to the need for individual green pepper traceability with big data, we identified fluorescence features in green pepper images that are imperceptible under natural light. Through mechanistic analysis, we determined that over 93% of these fluorescence features naturally arise during the growth process and remain stable. Leveraging feature point matching algorithms, we constructed a label-free tracking system that enabled the traceability of individual green peppers, achieving an 83% accuracy rate in line with commercial application standards. The integration of the indoor positioning system and fluorescence imaging system aligns with the overarching objective of this research, which is to elevate precision agriculture to a level where plant-to-plant accuracy is attainable. We have authored or co-authored 6 SCI papers for showing the new findings as well as presentations at 5 international academic conferences. These publications and conference contributions play a crucial role in amplifying the impact of our research endeavors.

Report

(3 results)
  • 2023 Annual Research Report
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (5 results)

All 2023

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (1 results)

  • [Journal Article] Macroscopic and Microscopic Characterization of Fluorescence Properties of Multiple Sweet Pepper Cultivars (Capsicum Annuum L.) Using Excitation-Emission Matrix and UV Induced Fluorescence Imaging.2023

    • Author(s)
      Huang, Z.; Takemoto, T.; Omwange, K.A.; Saito, Y.; Kuramoto, M.; Kondo, N
    • Journal Title

      Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

      Volume: 288 Pages: 122094-122094

    • DOI

      10.1016/j.saa.2022.122094

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Investigating the characteristics of fluorescence features on sweet peppers using UV light excitation2023

    • Author(s)
      Huang Zichen、Takemoto Tetsuyuki、Saito Yoshito、Omwange Ken Abamba、Konagaya Keiji、Hayashi Takahiro、Kondo Naoshi
    • Journal Title

      Photochemical & Photobiological Sciences

      Volume: 22 Issue: 10 Pages: 2401-2412

    • DOI

      10.1007/s43630-023-00459-5

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free technology for traceable identification of single green pepper through features in UV fluorescent images2023

    • Author(s)
      Takemoto Tetsuyuki、Huang Zichen、Omwange Ken Abamba、Saito Yoshito、Konagaya Keiji、Suzuki Tetsuhito、Ogawa Yuichi、Kondo Naoshi
    • Journal Title

      Computers and Electronics in Agriculture

      Volume: 211 Pages: 107960-107960

    • DOI

      10.1016/j.compag.2023.107960

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kondo, N. Monitoring Strawberry (Fragaria × Ananassa) Quality Changes during Storage Using UV-Excited Fluorescence Imaging.2023

    • Author(s)
      Huang, Z.; Omwange, K.A.; Saito, Y.; Kuramoto, M.; Kondo, N.
    • Journal Title

      Kondo, N. Monitoring Strawberry (Fragaria × Ananassa) Quality Changes during Storage Using UV-Excited Fluorescence Imaging.

      Volume: 353 Pages: 111553-111553

    • Related Report
      2023 Annual Research Report
  • [Presentation] Research progress of Japanese harvesting robot in facility agriculture,China, Invitational Lecture2023

    • Author(s)
      Zichen HUANG
    • Organizer
      The 7th World Intelligence Congress Intelligent Agriculture Summit Forum & International Conference on Intelligent Agriculture (ICIA2023)
    • Related Report
      2023 Annual Research Report

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Published: 2022-02-08   Modified: 2024-12-25  

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